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A phosphorous deficiency responsive phosphate transporter tapht1.6 and its coding gene and application

A transgenic cell line and coding technology, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of lack of whole genome sequence information, genome complexity, lack of systematic understanding of sequence information and functions, etc., to reduce soil environmental pollution, Increased growth rate, increased absorption effect

Active Publication Date: 2016-01-06
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of the wheat genome, the sequence information of the whole genome is still lacking, and there is still a lack of systematic understanding of the sequence information and function of the PHT1 phosphate transporter gene in wheat

Method used

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  • A phosphorous deficiency responsive phosphate transporter tapht1.6 and its coding gene and application
  • A phosphorous deficiency responsive phosphate transporter tapht1.6 and its coding gene and application
  • A phosphorous deficiency responsive phosphate transporter tapht1.6 and its coding gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, discovery of TaPHT1 protein and its coding gene

[0038] 根据大麦中HvPHT1(AAN37900,AY187020,AY187025,AAO72435,AAN37901,AAO72436,AAO72440,AM904733)和水稻中的OsPHT1(AAN39042,AAN39043,AAN39044,AAN39045,AAN39046,AAN39047,AAN39048,AAN39049,AAN39050,AAN39051,AAN39052)设计 Conserved primers, using the BAC library of wheat (wheat variety Xiaoyan 54) as a template, screened a single clone with amplified target fragment, after sequencing the BAC clone, obtained a homologous sequence of PHT1, the sequence was 1620bp long, without The intron presumably encodes a polypeptide consisting of 539 amino acid residues, named TaPHT1 protein.

[0039] The amino acid sequence of the TaPHT1 protein is sequence 2 in the sequence listing, and the nucleotide sequence of the gene encoding the protein is sequence 1 in the sequence listing.

[0040] Sequence 1 in the Sequence Listing can also be artificially synthesized.

Embodiment 2

[0041] Example 2. Functional complementation test of TaPHT1 protein and its coding gene

[0042] 1. Construction of yeast expression vector

[0043] Construct the expression vector of TaPHT1.6 expressed in yeast: according to the p112A1NE insertion site requirements, first connect the two ends of TaPHT1.6 with corresponding restriction sites to the T vector, and then insert TaPHT1.6 by restriction restriction Ligated into the insertion site of p112A1NE.

[0044] The specific process is as follows:

[0045] 1. Using the DNA molecule shown in sequence 1 in the artificially synthesized sequence list as a template, design a forward primer and a reverse primer according to the sequence of TaPHT1.6, and perform PCR amplification;

[0046] Forward primer: 5′-GTCGAATTC ATGGCGCGCGAGCAGCTGGA-3'; EcoRI restriction site was introduced into the forward primer;

[0047] Reverse primer: 5′-CTAGCGGCCGCTCACACGGGCACCGTCCTGG-3 Reverse primer introduces NotI restriction site.

[0048] PCR r...

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Abstract

The invention discloses a phosphate radical transport protein TaPHT1.6 with phosphorus deficiency response as well as a coding gene and application thereof. The protein provided by the invention is (a) or (b) as follows: (a), protein consisting of amino acid sequences as shown in a sequence 2 in a sequence table; and (b), protein obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence of the sequence 2, having the same function as the protein a and derived from the sequence 2. Experiments prove that absorption of yeast to phosphor is increased and phosphor nutritional status of the protein is improved by inducing a coding gene of the protein into a yeast phosphor absorption mutant, so that growth velocity of the protein is obviously increased.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a phosphorus-deficiency-responsive phosphate radical transporter TaPHT1.6, its coding gene and application. Background technique [0002] Phosphorus is one of the macronutrients necessary for plant growth. Phosphorus deficiency in the soil will seriously affect the growth and development of plants, resulting in reduced crop yields. Phosphorus fertilizer application is an important agronomic measure to ensure high yield of crops. my country's annual phosphate fertilizer consumption is about 10 million tons, which is one of the countries with the largest phosphate fertilizer consumption in the world. Phosphate rock is the most important raw material for the production of phosphate fertilizers, but phosphate rock resources are non-renewable resources. Based on the current annual consumption of phosphate fertilizer, the global phosphate rock resources will be depleted in the next few ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/19C12N15/11A01H5/00
CPCC07K14/415C12N15/8261
Inventor 童依平赵艳艳赵学强何雪马文英
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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