39 results about "Enterohemorrhagic e coli" patented technology

The invention discloses a detection kit and a detection method for 9 species of pathogenic organisms in marine products. The kit comprises Taq DNA polymerase with a concentration of 5U/muL and a PCR reaction solution, wherein the PCR reaction solution contains 10 millimols of Tris.HCl, 50 millimols of KCl, 25 millimols of MgCl2, 2.5 millimols of dNTP and primer pairs of the 9 species of pathogenic organisms. The kit and the method can synchronously detect the 9 species of pathogenic organisms and can detect salmonella with a concentration of 11 CFU/mL, staphylococcus aurei with a concentration of 2,000 CFU/mL, monocytogenes with a concentration of 120 CFU/mL, enterohemorrhagic Escherichia coli O157:H76 with a concentration of CFU/mL, comma bacillus with a concentration of 220 CFU/mL, Vibrio parahaemolyticus with a concentration of 60 CFU/mL, Vibrio vulnficus with a concentration of 9 CFU/mL and Vibrio alginolyicus with a concentration of 230 CFU/mL. The detection method short in detection time and simple and quick in operation, and can save a large amount of labor and financial resources and meet requirements for quick detection.

Compositions and methods for stimulating an immune response against a secreted enterohemorragic Escherichia coli (EHEC) antigen are disclosed. The compositions comprise EHEC cell culture supernatants.

The invention discloses a poly valency fuse type enterorrhagia property bacillus coli O157:H7 gene engineering vaccine, which comprises the following steps: adopting enterorrhagia property bacillus coli O157:H7 Vi antigen Shiga's toxin II; combining subunit, compact sticking element and III type secretory protein A; constructing fuse engineering bacteria through gene retooling method; proceeding high density ferment; proceeding a series of purity course; getting fuse protein molecule vaccine with high purity. This invention possesses simple craft, good repeatability and higher protein purity.

The invention belongs to the pharmaceutical biotechnology field, and relates to enterohemorrhagic E.coli O157:H7 Shiga toxin IIB epitope peptide and the application thereof. Four preferred polypeptides are derived from B-cell epitope of the subunit (Stx2A1) of EHEC O157:H7 Shiga toxin IIB epitope peptide. The product can be applied for preparing pharmaceuticals for the diagnosis and treatment of EHEC O157 infection and complications thereof.

The present invention provides methods and compositions for production of gram-negative bacterial mutants that are defective in intestinal colonization capacity and sensitive to infection by bacteriophage P1. Thus the present invention provides immunogenic compositions for the prevention or attenuation of food- and water-borne illnesses associated with ingestion of bacteria such as enterohemorrhagic Escherichia coli.

A set of oligonucleotide probe for detecting the enterohemorrhagic E. coli O157:H7 and cholera vibrio O139 is designed on the Shiga-like toxin generating gene stx1 or stx2 and the beta-glucuronidase gene uidA of said enterohemorrhagic E coli O157:H7 and the enterotoxin A subset (ctxA), toxicity coordinate pilus A subset (tcpA) and glucosyltransferase (LPS gt) genes of cholera vibrio O139, and has high sensibility and specificity. It can be used to detect the enterohemorrhagic E. coli O157:H7, cholera vibrio O139 and other pathogenic genes from the specimen.

The invention relates to a spice plant extract, and particularly relates to an ethanolic extract of cinnamons and an application thereof in preparing food preservatives; a natural food preservative is prepared by combining the ethanolic extract of cinnamons with a food additive; and the ethanolic extract of cinnamons provided by the invention has a good sterilization effect on various typical food-borne pathogenic bacteria, especially has a significant sterilization effect on the pathogenic bacteria O157: H7 of EHEC (enterohaemorrhagic escherichia coli, EHECO157: H7) food poisoning continuously occurring around the world in recent years, and has no side effects on food, therefore, the ethanolic extract of cinnamons is a natural food preservative which is safe and has no side effects; the ethanolic extract of cinnamons is widely applied to the decomposition prevention of products such as fresh vegetables and fruits and beef hamburgers and the like; and in the aspect of freshness retaining, the ethanolic extract of cinnamons not only can effectively prolong the shelf life of food, but also can effectively prevent the occurrence of bacterial food poisoning and effectively ensure the safety of food, therefore, the ethanolic extract of cinnamons plays an important role in the field of food health and safety.

The invention discloses a method for detecting live enterohemorrhagic escherichia coli O157: H7. The method solves the problem that the existing real-time fluorescence PCR technology cannot effectively distinguish live or dead enterohemorrhagic escherichia coli thereby leading to overestimation of a pathogenic bacterium risk. The method comprises the following steps of pre-treating a liquid sample needing to be detected, adding propidium monoazide (PMA) into the pre-treated liquid sample, controlling a PMA final concentration to 15-20 micrograms per milliliter, carrying out incubation in the dark at a normal temperature, carrying out halogen tungsten lamp radiation treatment, extracting a genome DNA of the treated sample, utilizing the extracted sample genome DNA as a template, carrying out real-time fluorescence PCR quantitative detection of object enterohemorrhagic escherichia coli in the sample, wherein primers and fluorescent label probes used by the real-time fluorescence PCR quantitative detection are shown in the formula of SEQ ID NO.1. The method has short operation time of about 1.5h and can be widely used in the field of food-borne pathogenic bacterium detection.

The invention discloses a gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in a water body. The method is characterized in that specific gene segments of the pathogenic microorganisms are amplified simultaneously by adopting multiple polymerase chain reactions (PCR), and then, a detection result is obtained through gene chip hybridization and chip scanning image analysis. The detected thirteen pathogenic microorganisms comprise escherichia coli, Enterohemorrhagic Escherichia coli O157:H7, legionella pneumophila, salmonella enteritidis, Shigella, staphylococcus aureus, Listeria monocytogenes, Helicobacter Pylori, mycobacterium tuberculosis, Klebsiella pneumonia, vibrio cholera, Bacillus anthracis and Yersinia pestis.

The present invention provides a detection kit for E.coli stx2 gene. The E.coli stx2 gene detection kit includes two pairs of primers designed based on the loop-mediated constant temperature amplification technology with the stx2 gene as the target gene. : inner primer FIP/BIP and outer primer F3/B3. The enterohaemorrhagic Escherichia coli stx2 gene detection kit of the present invention has more comprehensive detection effect and low missed detection rate.

The invention discloses preparation and applications of a nucleic acid lateral flow test strip kit for detecting EHEC O157, and belongs to the technical field of bacterial examination. According to the preparation and applications, a high-sensitivity and high-specificity method for polymerase chain reaction in a nucleic acid test is combined with an immune colloidal gold quick testing technology in immunological detection, specific amplification is carried out on a target DNA by virtue of a unique marked primer pair, and an amplification product is combined with a gold labeled antibody fixed on a test paper strip so as to form a stable and visible test band and a stable and visible quality control band, and thus the quick and accurate testing on Escherichia coli O157:H7 can be realized.

The invention belongs to the technological field of medicine bioengineering, which more particularly relates to a monoclonal antibody neutralizing enterohemorrhagic escherichia coli O157:H7 shiga toxin II, Fab antibody sequences and the application. The monoclonal antibody is prepared by hybridoma cell lines with the preservation number of CCTCC: C200822. The antibody of the invention can be used for preparing medicines for diagnosing and treating EHEC O157 infections and the complicating diseases thereof.

The invention relates to the technical field of a biological preparation, and discloses lactobacillus gasseri for resisting enterohemorrhagic escherichia coli, an application of the lactobacillus gasseri, a composite probiotic preparation for poultry, prepared from the lactobacillus gasseri and an application of the composite probiotic preparation. For the lactobacillus gasseri (Lactobacillus gasseri) YD2-2 provided by the invention, the preservation No. is CCTCC NO:M 2019340, the lactobacillus gasseri particularly has efficient bacterial inhibition for the enterohemorrhagic escherichia coli,after the lactobacillus gasseri is mixed and blended with other bacterial strains, the composite probiotic preparation for poultry for resisting enterohemorrhagic escherichia coli can be prepared. Thepreparation can promote the growth of animals, the microbiological environment of intestines of the animals can be improved, the development of intestine mucosa can be promoted, the solubility IgA content and the serum IgG content of the intestine mucosa can be increased, the long-term sub-therapeutic dose and ultrahigh dose of antibiotics for preventing and treating animal diseases in feeds canbe reduced, and the damage in respects of bacterial drug resistance, drug residues of antibiotics, food safety and the like caused by indiscriminate use of the antibiotics can be avoided.

The invention provides a nano PCR (Polymerase Chain Reaction) kit for simultaneously detecting enterohemorrhagic escherichia coli (EHEC) O45 and O145 and application thereof and relates to the technical field of detection of the enterohemorrhagic escherichia coli. The kit comprises a first primer pair and a second primer pair; the first primer pair comprises primers as shown by SEQ ID No: 3 and SEQ ID No: 4; and the second first primer pair comprises primers as shown by SEQ ID No: 7 and SEQ ID No: 8. The invention further provides the application of the kit to detection of the enterohemorrhagic escherichia coli O45 and O145 in a manner of not taking diagnosis as a target. The nano PCR kit provided by the invention can be used for rapidly and specifically simultaneously detecting two bloodserum types of EHEC in a sample and has a good application prospect.

The invention relates to a primer and a probe for simultaneously detecting enterohemorrhagic escherichia coli O157 and O104. The invention also relates to a dual real-time fluorescence PCR detection method for simultaneously detecting enterohemorrhagic escherichia coli O157 and O104 in food. The method comprises a step of using a specific oligonucleotide primer and a probe aiming at detection of enterohemorrhagic escherichia coli O157 and O104. The invention also relates to a dual real-time fluorescence PCR detection kit for detecting enterohemorrhagic escherichia coli O157 and O104 in the food, and the kit comprises the specific oligonucleotide primer and the probe. The dual real-time fluorescence PCR detection method can simultaneously detecting enterohemorrhagic escherichia coli O157 and O104 in a simple, rapid, specific, and sensitive mode.