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Bacterially formed microcin s, a new antimicrobial peptide, effective against pathogenic microorganisms, e.g. enterohemorrhagic escherichia coli (EHEC)

An antimicrobial, microcin technology, applied in the field of coating dressing materials, producing and using the polypeptide, treating or preventing microbial infection, primers and probes, functional gastrointestinal diseases or treating tumors, can Address issues such as limited treatment options

Inactive Publication Date: 2014-04-09
SYMBIOGRUPPE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the emergence of pathogens resistant to these traditional antibiotics poses a huge problem
Therefore, the remaining treatment options are even more limited

Method used

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  • Bacterially formed microcin s, a new antimicrobial peptide, effective against pathogenic microorganisms, e.g. enterohemorrhagic escherichia coli (EHEC)
  • Bacterially formed microcin s, a new antimicrobial peptide, effective against pathogenic microorganisms, e.g. enterohemorrhagic escherichia coli (EHEC)
  • Bacterially formed microcin s, a new antimicrobial peptide, effective against pathogenic microorganisms, e.g. enterohemorrhagic escherichia coli (EHEC)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0242] Example 1: Bacterial Strains and Growth Conditions

[0243] Bacterial strains used in this paper are listed in Table 3.

[0244] Table 3: Bacterial strains used

[0245]

[0246]

[0247]

[0248] a DSM17252

Embodiment 2

[0249] Example 2: Identification of a microcin-encoding gene cluster in G3 / 10 of E. coli

[0250] The genomes of six E. coli species: E. coli G1 / 2, G3 / 10, G4 / 9, G5, G6 / 7 and G8 were sequenced. Annotation shows that there are no known microcins in E. coli G3 / 10. Escherichia coli G3 / 10 contains a large conjugative plasmid pSYM1 ( figure 1 ). The plasmid is 99% identical to the plasmid pMAS2027 of the uropathogenic E. coli isolate. Furthermore, it contained a 10 kb insert, but BLAST analysis only revealed uncharacterized and unnamed genes. In order to identify the source of the bactericidal effect, an attempt was made to cure the strain E. coli G3 / 10 by its large plasmid pSYM1. Despite many common treatments such as mitomycin C (mitomycin C) or heat treatment, the strains could not be treated. Thus, plasmid pSYM1 was transferred to E. coli G4 / 9 by conjugation. To allow selection of conjugants, a first ampicillin resistance cassette was integrated into pSYM1, resulting in p...

Embodiment 3

[0251] Example 3: Functional Characterization of Microcin S and Interpretation of Its Autoimmunity

[0252] Bacterial attachment is a critical first step in many infectious diseases. Therefore, a test system that quantifies the inhibition of attachment of human intestinal epithelial cells is suitable for demonstrating beneficial effects on the host. In the first experiment, a confluent monolayer of CACO-2 was pre-incubated at a 100:1 MOI of E. coli to host cells using the bacterial test strain EcN, E. coli G3 / 10, E. coli G4 / 9, or E. coli G4 / 9pAZ6 or LOVO cells. After two hours of incubation, cells were washed and infected with EPEC E2348 / 69 using a 100:1 MOI of EPEC to host cells. E. coli G3 / 10 and E. coli G4 / 9pAZ6 were able to inhibit EPEC attachment similarly to EcN. showed that EcN attachment inhibition depends on strain microcin activity. Attachment efficiency in % is expressed as attachment of EPECs compared to attachment without any pre-incubation (negative control),...

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Abstract

The present invention relates to a new isolated polypeptide nominated microcin S, isolated nucleic acid molecules encoding the microcin S polypeptide and primers and probes hybridizing to the nucleic acid molecules. The invention also relates to plasmids and cells comprising the nucleic acid molecules, an antibody binding to the polypeptide, compositions as well as methods for producing and using the polypeptides. The present invention further relates to medical uses for treating or preventing microbial infections, functional gastrointestinal disorders or treating a tumor. The invention further relates to a method for preserving food and a method for coating dressing material.

Description

technical field [0001] The present invention relates to isolated polypeptides, isolated nucleic acid molecules encoding microcin S (microcin S) polypeptides, and primers and probes that hybridize to said nucleic acid molecules. The invention also relates to plasmids and cells comprising said nucleic acid molecules, antibodies that bind said polypeptides, compositions and methods for producing and using said polypeptides. The invention further relates to medical uses for the treatment or prevention of microbial infections, functional gastrointestinal disorders or the treatment of tumors. The invention further relates to methods for preserving food and methods for coating dressing materials. Background technique [0002] Microcins are ribosomally synthesized low molecular weight antimicrobial peptides. Microcin synthesis produced by enterobacteria (mainly Escherichia coli) is dramatically activated under stress conditions such as nutrient limitation. Microcin exerts potent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/74A01N63/50
CPCA23B4/22C07K14/245A23C3/08A23L3/34635A01N47/44A01N57/16C07K16/1232A61K38/00A61P1/00A61P1/04A61P17/00A61P31/02A61P31/04A61P35/00Y02A50/30A01N63/50A01N63/00A61K9/70C12N15/00C12Q1/00A23V2002/00
Inventor 弗洛里安·贡策安克·琼蒂格屈特·齐默尔曼
Owner SYMBIOGRUPPE
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