Lactobacillus gasseri for resisting enterohemorrhagic escherichia coli, application of lactobacillus gasseri, composite probiotic preparation for poultry, prepared from lactobacillus gasseri and application of composite probiotic preparation

A technology of Lactobacillus gasseri and compound probiotics, applied in the direction of bacteria, Lactobacillus, and other applications used in food preparation, can solve the problems of poor vaccine control effects, drug residues, drug resistance, etc., and improve intestinal Mucosal immune level, improvement of resistance, effect of enhancing resistance

Active Publication Date: 2019-08-20
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many serotypes of Escherichia coli, which have the characteristics of local epidemic serotypes. The vaccines currently developed usually have a single serotype, and the use of vaccines is not effective; the use of antibiotics will cause drug residues and drug resistance

Method used

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  • Lactobacillus gasseri for resisting enterohemorrhagic escherichia coli, application of lactobacillus gasseri, composite probiotic preparation for poultry, prepared from lactobacillus gasseri and application of composite probiotic preparation
  • Lactobacillus gasseri for resisting enterohemorrhagic escherichia coli, application of lactobacillus gasseri, composite probiotic preparation for poultry, prepared from lactobacillus gasseri and application of composite probiotic preparation
  • Lactobacillus gasseri for resisting enterohemorrhagic escherichia coli, application of lactobacillus gasseri, composite probiotic preparation for poultry, prepared from lactobacillus gasseri and application of composite probiotic preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Isolation and identification of Lactobacillus gasseri YD2-2:

[0034] 1) Purchase healthy free-range chickens from a chicken farm in Xianning, dissect the intestines, and take the contents of the intestines in a sterile environment. After shaking and mixing, they are diluted in multiples, and the appropriate dilution is selected and spread on the lactic acid bacteria selective medium MRS agar culture. On the substrate, culture anaerobically at 37°C for 24-48h, pick a single colony on the plate, streak and purify it on the MRS medium plate several times, and cultivate it aerobically at 37°C to obtain pure colonies. Colony morphology observation and Gram staining were performed on the purified strains, and Gram-positive bacilli were selected. The colony diameter of the bacterial strain of the present invention is 0.5-1mm on the MRS medium, milky white, raised, round in shape, smooth in surface and facultative anaerobic. The strain is Gram-positive, round-ended straight r...

Embodiment 2

[0043] Effect of Lactobacillus gasseri YD2-2 on immune cytokines in mice:

[0044] Select 4-week-old female Kunming rats as experimental animals, and use the method of gavage for 1 week, once a day, each time with 0.2ml of YD2-2 fermentation broth, the amount of bacteria contained in the fermentation broth is 10 8 ~10 9 cfu / ml. Normal feeding for one week before gavage, and normal feeding for one week after gavage. The whole experiment was 21 days. After the experiment, eyeball blood was collected to measure cytokines, and the effect of YD2-2 on mouse IFNβ and IL-1β cytokines was determined by fluorescent quantitative PCR, and GAPDH was used as an internal reference gene. The results of fluorescence quantitative detection of IFNβ and IL-1β are as follows:

[0045] Table 2 Effect of Lactobacillus gasseri YD2-2 on immune cytokines in mice.

[0046]

[0047] Assume that the amplification efficiencies of both the target gene and the reference gene are close to 100%, and the...

Embodiment 3

[0053] A compound probiotic preparation for anti-enteritis Escherichia coli infection, including Bacillus subtilis, Bacillus coagulans, Bacillus amyloliquefaciens, Lactobacillus gasseri and Lactobacillus plantarum; the number of viable bacteria of Bacillus subtilis is 10 9 CFU / g, the number of viable Bacillus coagulans is 10 8 CFU / g, the number of live bacteria of Bacillus amyloliquefaciens is 10 8 CFU / g, the viable count of Lactobacillus gasseri is 10 8 CFU / g, the number of viable Lactobacillus plantarum is 10 8 CFU / g.

[0054] The Bacillus amyloliquefaciens is Bacillus amyloliquefaciens H6, CCTCC NO: M2013249; the Lactobacillus plantarum is Lactobacillus plantarum ZN-3, CCTCC NO: M 2017286, and the Lactobacillus gasseri is Lactobacillus gasseri YD2-2, CCTCC NO: M2019340, other strains were purchased from commercial channels.

[0055] The composite probiotic preparation prepared in Example 3 is used in the following examples.

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Abstract

The invention relates to the technical field of a biological preparation, and discloses lactobacillus gasseri for resisting enterohemorrhagic escherichia coli, an application of the lactobacillus gasseri, a composite probiotic preparation for poultry, prepared from the lactobacillus gasseri and an application of the composite probiotic preparation. For the lactobacillus gasseri (Lactobacillus gasseri) YD2-2 provided by the invention, the preservation No. is CCTCC NO:M 2019340, the lactobacillus gasseri particularly has efficient bacterial inhibition for the enterohemorrhagic escherichia coli,after the lactobacillus gasseri is mixed and blended with other bacterial strains, the composite probiotic preparation for poultry for resisting enterohemorrhagic escherichia coli can be prepared. Thepreparation can promote the growth of animals, the microbiological environment of intestines of the animals can be improved, the development of intestine mucosa can be promoted, the solubility IgA content and the serum IgG content of the intestine mucosa can be increased, the long-term sub-therapeutic dose and ultrahigh dose of antibiotics for preventing and treating animal diseases in feeds canbe reduced, and the damage in respects of bacterial drug resistance, drug residues of antibiotics, food safety and the like caused by indiscriminate use of the antibiotics can be avoided.

Description

technical field [0001] The invention relates to the technical field of biological preparations, in particular to a Lactobacillus gasseri resistant to enterohaemorrhagic Escherichia coli and a prepared compound probiotic preparation for poultry and its application. Background technique [0002] Intestinal microorganisms and the host have formed a stable symbiotic relationship during the long-term evolution process. The number of intestinal microorganisms is huge and there are many types. In a healthy state, various bacteria interact and check and balance each other to maintain the intestinal microecology. balance. However, with the intensive development of the livestock and poultry breeding industry and the abuse of antibiotics in the breeding process, a great stress response is produced on the animal body; Breeding will lead to the imbalance of micro-ecology inside and outside the animal body, the decline of immune function and production performance, cause serious economic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A61K35/747A61P31/04A23K10/18C12R1/225
CPCC12N1/20A61K35/742A61K35/747A61P31/04A23K10/18C12R2001/225C12N1/205A23V2400/145A23V2400/169A61K2300/00
Inventor 金梅林杨丽康超孙小美
Owner HUAZHONG AGRI UNIV
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