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Lactobacillus gasseri against enterohemorrhagic Escherichia coli and the prepared compound probiotic preparation for poultry and application thereof

A technology of Lactobacillus gasseri and Escherichia coli, applied in the directions of bacteria, Lactobacillus, application, etc. used in food preparation, can solve the problems of poor vaccine control effect, drug residues, drug resistance, etc., and improve the intestinal mucosa. The effect of immunity level, improving resistance, strengthening resistance

Active Publication Date: 2020-12-22
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many serotypes of Escherichia coli, which have the characteristics of local epidemic serotypes. The vaccines currently developed usually have a single serotype, and the use of vaccines is not effective; the use of antibiotics will cause drug residues and drug resistance

Method used

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  • Lactobacillus gasseri against enterohemorrhagic Escherichia coli and the prepared compound probiotic preparation for poultry and application thereof
  • Lactobacillus gasseri against enterohemorrhagic Escherichia coli and the prepared compound probiotic preparation for poultry and application thereof
  • Lactobacillus gasseri against enterohemorrhagic Escherichia coli and the prepared compound probiotic preparation for poultry and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Isolation and identification of Lactobacillus gasseri YD2-2:

[0034] 1) Purchase healthy free-range chickens from a chicken farm in Xianning, dissect the intestines, and take the contents of the intestines in a sterile environment. After shaking and mixing, they are diluted in multiples, and the appropriate dilution is selected and spread on the lactic acid bacteria selective medium MRS agar culture. On the substrate, culture anaerobically at 37°C for 24-48h, pick a single colony on the plate, streak and purify it on the MRS medium plate several times, and cultivate it aerobically at 37°C to obtain pure colonies. Colony morphology observation and Gram staining were performed on the purified strains, and Gram-positive bacilli were selected. The colony diameter of the bacterial strain of the present invention is 0.5-1mm on the MRS medium, milky white, raised, round in shape, smooth in surface and facultative anaerobic. The strain is Gram-positive, round-ended straight r...

Embodiment 2

[0043] Effect of Lactobacillus gasseri YD2-2 on immune cytokines in mice:

[0044] Select 4-week-old female Kunming rats as experimental animals, and use the method of gavage for 1 week, once a day, each time with 0.2ml of YD2-2 fermentation broth, the amount of bacteria contained in the fermentation broth is 10 8 ~10 9 cfu / ml. Normal feeding for one week before gavage, and normal feeding for one week after gavage. The whole experiment was 21 days. After the experiment, eyeball blood was collected to measure cytokines, and the effect of YD2-2 on mouse IFNβ and IL-1β cytokines was determined by fluorescent quantitative PCR, and GAPDH was used as an internal reference gene. The results of fluorescence quantitative detection of IFNβ and IL-1β are as follows:

[0045] Table 2 Effect of Lactobacillus gasseri YD2-2 on immune cytokines in mice.

[0046]

[0047] Assume that the amplification efficiencies of both the target gene and the reference gene are close to 100%, and the...

Embodiment 3

[0053] A compound probiotic preparation for anti-enteritis Escherichia coli infection, including Bacillus subtilis, Bacillus coagulans, Bacillus amyloliquefaciens, Lactobacillus gasseri and Lactobacillus plantarum; the number of viable bacteria of Bacillus subtilis is 10 9 CFU / g, the number of viable Bacillus coagulans is 10 8 CFU / g, the number of live bacteria of Bacillus amyloliquefaciens is 10 8 CFU / g, the viable count of Lactobacillus gasseri is 10 8 CFU / g, the number of viable Lactobacillus plantarum is 10 8 CFU / g.

[0054] The Bacillus amyloliquefaciens is Bacillus amyloliquefaciens H6, CCTCC NO: M2013249; the Lactobacillus plantarum is Lactobacillus plantarum ZN-3, CCTCC NO: M 2017286, and the Lactobacillus gasseri is Lactobacillus gasseri YD2-2, CCTCC NO: M2019340, other strains were purchased from commercial channels.

[0055] The composite probiotic preparation prepared in Example 3 is used in the following examples.

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Abstract

The invention relates to the technical field of biological preparations, and discloses a Lactobacillus gasseri resistant to enterohemorrhagic Escherichia coli, a prepared compound probiotic preparation for poultry and an application thereof. Lactobacillus gasseri provided by the invention ( Lactobacillus gasseri ) YD2‑2, the preservation number is CCTCC NO: M 2019340, especially for enterohaemorrhagic Escherichia coli with high antibacterial activity, after mixing it with other strains, it can be prepared into anti-enterohemorrhagic poultry compound probiotic preparation , the preparation can promote the growth of animals, improve the intestinal micro-ecological environment of animals, promote the development of intestinal mucosa, increase the content of soluble IgA in intestinal mucosa and the content of serum IgG, and reduce the long-term effect of antibiotics in feed for the prevention and treatment of animal diseases Sub-therapeutic dosage and ultra-high dosage are added to avoid the harm of bacterial resistance, antibiotic residues and food safety caused by the abuse of antibiotics in feed.

Description

technical field [0001] The invention relates to the technical field of biological preparations, in particular to a Lactobacillus gasseri resistant to enterohaemorrhagic Escherichia coli and a prepared compound probiotic preparation for poultry and its application. Background technique [0002] Intestinal microorganisms and the host have formed a stable symbiotic relationship during the long-term evolution process. The number of intestinal microorganisms is huge and there are many types. In a healthy state, various bacteria interact and check and balance each other to maintain the intestinal microecology. balance. However, with the intensive development of the livestock and poultry breeding industry and the abuse of antibiotics in the breeding process, a great stress response is produced on the animal body; Breeding will lead to the imbalance of micro-ecology inside and outside the animal body, the decline of immune function and production performance, cause serious economic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/742A61K35/747A61P31/04A23K10/18C12R1/225
CPCC12N1/20A61K35/742A61K35/747A61P31/04A23K10/18C12R2001/225C12N1/205A23V2400/145A23V2400/169A61K2300/00
Inventor 金梅林杨丽康超孙小美
Owner HUAZHONG AGRI UNIV
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