Enterohemorrhagic Escherichia coli stx2 gene detection kit and its application method

Abstract

The present invention provides a detection kit for E.coli stx2 gene. The E.coli stx2 gene detection kit includes two pairs of primers designed based on the loop-mediated constant temperature amplification technology with the stx2 gene as the target gene. : inner primer FIP / BIP and outer primer F3 / B3. The enterohaemorrhagic Escherichia coli stx2 gene detection kit of the present invention has more comprehensive detection effect and low missed detection rate.

Description

technical field [0001] The invention relates to biological detection reagents, in particular to an enterohaemorrhagic Escherichia coli stx2 gene detection kit and a use method thereof. Background technique [0002] Enterohemorrhagic Escherichia coli (EHEC) infection broke out in Germany in May 2011. According to the report of the World Health Organization, as of June 5, Germany had reported 630 HUS cases and 15 deaths; 1601 EHEC infection cases and 6 deaths example. In addition, 12 European countries including Austria, Czech Republic, Sweden, Denmark, Netherlands, Poland, France, Switzerland, Norway, Spain, Luxembourg and the United Kingdom reported a total of 31 cases of HUS (1 death) and 73 cases of EHEC infection. Two cases of HUS were reported in the United States. [0003] Enterohaemorrhagic E. coli infection is a zoonotic disease that primarily causes abdominal cramping and mild diarrhea, some cases bloody (hemorrhagic enteritis). Most patients recover within 10 day...

AI Technical Summary

Problems solved by technology

[0006]The traditional detection method of enterohemorrhagic Escherichia coli is far from meeting the requirements of modern detection due to its shortcomings such as long detection cycle, complicated procedures, and various reagents required

The pathogenic nucleic acid detection technology represented by polymerase chain reaction (PCR) technology has some problems in practical application, such as ordinary polymerase chain reaction (PCR) technology requires special instruments, and there are easy cross-contamination and cumbersome operation process Shortcomings

Although the fluorescent real-time quantitative polymerase chain reaction (real time PCR) technology has better solved the problem of cross-contamination and simplified the operation process, it requires more complex quantitative measurement instruments, so it is not suitable for on-site rapid detection

Moreover, the cost of fluorescent probes in real-time quantitative polymerase chain reaction PCR technology is relatively high, which increases the difficulty of popularization and application.

Immunological detection technology is fast, simple and low-cost, but requires high-quality and high-stability monoclonal antibodies. Otherwise, due to insufficient accuracy, it can only be used as an auxiliary detection method at present.

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