Engineering lambda bacteriophage capable of specifically killing enterohemorrhagic Escherichia coli and construction method and application of engineering lambda bacteriophage
An enterohemorrhagic, Escherichia coli technology, applied in the direction of bacteriophage, virus/phage, microorganism-based methods, etc., to achieve high specific killing effect and overcome the effect of broad-spectrum bactericidal
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0039] 1. Genetic engineering of lambda bacteriophage
[0040] The lyase gene of the lambda phage is knocked out and the CRIS PR-Cas3 system-related genes Cas3, CasA, CasB, CasC, CasD, CasE, Cas1, Cas2 and synthetic CrRNA are inserted into the lambda phage to obtain an engineered lambda phage, The CRISPR-Cas3 system was integrated into the host enterohemorrhagic Escherichia coli through lysogeny of λ phage, and the complementary base sequence was mediated by CrRNA to perform specific bactericidal function. The nucleotide sequence of the CRISPR-Cas3 system related gene is as follows: Shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 and SEQ ID NO:8, The nucleotide sequence of the CrRNA gene is shown in SEQ ID NO:9.
[0041] The specific construction process of the engineered lambda phage is:
[0042] Step S1: Inducing the release of lysogenic lambda phage
[0043] The lysogenic bacteria of λ phage are enriched and cultivated, ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com