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Method for intensifying functions of cytokine-induced killer cells

A technology of killing cells and cytokines, applied in animal cells, vertebrate cells, blood/immune system cells, etc., can solve the problems of slowing down the growth of lung tumors, enhancing the curative effect, and improving, so as to prolong the overall survival time and enhance the specificity. The effect of sexual killing

Inactive Publication Date: 2015-03-25
深圳市金佳禾生物医药湖北有限公司
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Problems solved by technology

Jiang Min et al [Jiang Min, Qin Xia, Hu Qinghai, et al. SOCS 1 gene silencing enhances the therapeutic effect of DO vaccine on animal models of lung cancer [J]. Modern Immunology, 2007, 27 (4): 294-299] found that , after tumor-bearing mice received vaccine treatment, although DCs without SOCS1 silencing also had a certain curative effect, silencing SOCS1 could significantly enhance the curative effect, and the growth of lung tumors was significantly slowed down. The specificity of killing is greatly improved, and the secretion of Thl-type cytokines IFN-γ and TNF-α is also further increased

Method used

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  • Method for intensifying functions of cytokine-induced killer cells
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  • Method for intensifying functions of cytokine-induced killer cells

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specific Embodiment 1

[0127] (1). Conditions of experimental mice: BALB / C (null) nude mice, 3-4 weeks old, purchased from Guangdong Experimental Animal Center, fed in a cage free of special pathogens;

[0128] (2). After the cultured MCF-7 cells (ATCC) were washed 3 times with PBS, the cell density was adjusted to 1×10 7 / mL, each mouse was subcutaneously inoculated with 200 μL of the above-mentioned MCF-7 cells, and 8 days after inoculation, the formation of tumors was observed with the naked eye.

[0129] (3). The mice with tumors were randomly divided into PBS, DC-CIKs (NO GH03), DC-CIKs (GH03) cell groups, 10 mice in each group, and the total number of cells injected intravenously was 2×10 6 , injected every other day, and re-injected with the same method for 2 consecutive times, the total number of cells was 2×10 6 , to observe tumor growth in mice.

[0130] (4). Measure the length and width of the tumor with a vernier caliper every two days, and calculate the volume of the tum...

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Abstract

The invention provides a method for intensifying functions of cytokine-induced killer cells. The method comprises the following steps: analyzing and obtaining an epitope through a bioinformatics analysis method, fusing the epitope and Ii-Kye into an epitope peptide, anchoring the epitope peptide on MHC-II molecules on the surfaces of dendritic cells, and infecting the dendritic cells with recombinant adenovirus rAd-RNAi-SOCS1; and further carrying out co-cultivation on the dendritic cells and cytokine-induced killer cells, and treating tumor-bearing mice with co-cultivated cells, so that the processed dendritic cells are capable of activating the cytokine-induced killer cells, and intensifying the significant killing functions of the cytokine-induced killer cells on related tumors. The invention provides a method for treating tumors employing activated cells; and the core technology of the method has reference significance on treatment of other viroses.

Description

technical field [0001] The invention relates to a method for preparing immune cells, in particular to a method for strengthening the function of cytokine-induced killer cells. Background technique [0002] The problem of cancer is becoming more and more severe, and the prevention and control of cancer are facing great challenges. The International Cancer Research Center of the World Health Organization believes that the number of cancer cases will increase at an average annual rate of 3% to 5% in the future. It is estimated that there will be 20 million new cancer cases and 14 million deaths in the world by 2020. According to the "Annual Report on Cancer Registration in China in 2012", the general situation of cancer incidence and mortality in my country is: 286 people per 100,000 people suffer from cancer, and the incidence of cancer increases gradually with the age of the population, especially for those over 50 years old. The number of cancers has risen sharply, and over ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/12A61K35/14A61K35/26A61K39/00A61P37/04A61P35/00C12N5/10C12N5/0783
CPCA61K35/17A61K35/761A61K39/0011A61K2039/5154A61K2039/545A61K2039/572A61K2039/585A61K2300/00
Inventor 郑义都会李智龙肖艳归
Owner 深圳市金佳禾生物医药湖北有限公司
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