36 results about "Cytokine-induced killer cell" patented technology

The invention discloses a preparation method of human cytokine-induced killer cells, comprising the following steps: coating a cell culture flask with a coating buffer containing effective amount of fusion protein and human CD3 monoclonal antibody before culturing precursor cells of human CIK cells, and adding the human CD3 monoclonal antibody in the whole process of inducing and culturing the human CIK cells, wherein the fusion protein is human intercellular adhesion molecule-1 functional domain and human fibronectin functional domain fusion protein, and the concentration of the human CD3 monoclonal antibody in the cell culture solution is lower than the concentration of the human CD3 monoclonal antibody in the coating buffer. According to the invention, ex-vivo expansion efficiency of peripheral blood mononuclear cells and the proportion of CD3 / CD56 double positive cells in the CIK cells are significantly raised, the cytotoxicity activity of the CIK cells is enhanced, thus the effect of cellular immunity treatment is raised.

A process for producing cytokine-induced killer cells, comprising the steps of (a) culturing a cell mass that contains cells capable of being differentiated into CD3-positive CD56-positive cells and / or CD3-positive CD56-positive cells in the presence of a CD3 ligand, (b) culturing the cell mass produced in step (a) in the absence of a CD3 ligand, and (c) culturing the cell mass produced in step (b) in the presence of a CD3 ligand; and others.

Disclosed are: a method for producing a cell mass containing a cytokine-induced killer (CIK) cell, which is characterized by comprising the step of culturing a cell mass containing a cell that can be differentiated into a CIK cell in the presence of a fragment of a fibronectin; and others.