Preparation of high efficiency immune active cell and method of using for anti tumour

Abstract

This invention relates to producing of high efficiency immune cell group and method of its using to tumor preventing, it belongs to biotechnology field. Dendritic cell is induced by oligonucleotide and cytokine, cell is induced and wounded by the cytokine, then dendritic cell and wounded cell are mixed with certain ratio to get new kind cell group that cultured by immune effect cell group dendritic that induced by oligonucleotide and the wounded cell. Compared to cell group cultured by wounded cell and dendritic cell, the proliferation activity is stronger. The cell poisonous activity is higher than the wounded cell. The oligonucleotide can be artificially synthesized. Immune cell group that anti cancer activity is higher than the wounded cell can also be induced to patient that operation opportunity is gone and tumor antigen is hard to get. It can be used to self-body tumor curing, chemical treatment and assistant curing to radiotherapy.

Description

Technical field [0001] The invention belongs to the field of biotechnology. In particular, it relates to a method for preparing a cell group obtained by co-cultivating anti-tumor immune active cells-oligonucleotide-induced dendritic cells and cytokine-induced killer cells. Background technique [0002] Malignant tumors are currently one of the main diseases that endanger human health. In countries where infectious diseases are controlled, cardiovascular and cerebrovascular diseases and malignant tumors have become the first and second causes of death respectively. According to statistics in 2005, malignant tumors were the first cause of death among urban residents in my country, and it was the second cause of death among rural residents. [0003] In the treatment of tumors, immunotherapy has become the most important and promising treatment besides surgery, chemotherapy and radiotherapy. In the past 20 years, there have been great developments in tumor-specific active immunothera...

AI Technical Summary

Problems solved by technology

Co-injection of CpG-ODN and antigen-stimulated DC cells into mice with colon cancer can significantly improve the anti-tumor activity of DC, and the ability of systemic administration to inhibit tumor growth is stronger than that of 5-FU and leucovorin (Sipos B , Sting G, 2002), however, the direct injection of oligonucleotides in the body requires a large dose and there are side effects such as tissue necrosis and bleeding after continuous injection for a week

[0007] The preparation of tumor antigen-shocked DCIK cells must undergo surgery and other methods to obtain fresh tumor tissue specimens to extract antigens. In clinical practice, a considerable number of patients have lost the opportunity for surgery, and patients who have undergone radiotherapy and chemotherapy are difficult to obtain tumor antigens.

Moreover, in order to induce a stronger immune effect, a large dose of CpG-ODN is injected, and the side effects are also relatively large.

Therefore, to improve the anti-tumor immune effector cells in the prior art in terms of proliferation performance, cytotoxic activity, and specific recognition and attack activity on tumor cells, a new anti-tumor immune effector cell and its preparation method have been developed, called for realistic needs