Kit for detecting 10 respiratory tract infection pathogens and application method thereof

Abstract

The invention provides a kit for detecting 10 respiratory tract infection pathogens and an application method thereof. The kit comprises primers and a TaqMan probe, wherein the primers are used for amplifying the 10 respiratory tract infection pathogens which include mycoplasma pneumoniae, chlamydia pneumoniae, legionella pneumophila, bordetella pertussis, rhinovirus, respiratory tract adenovirus, influenza A virus, influenza B virus, respiratory syncytial virus and parainfluenza virus. The application method includes: mixing 2 microliters of sample DNA / RNA co-extraction template, 20 microliters of RT-PCR buffer, 1 microliter of mixed enzyme liquid and 2 microliter of primer-probe mixed liquid to perform PCR amplification reaction. The kit uses the Taqman probe fluorescent PCR technology to detect the 10 common respiratory tract infection pathogens, a detection result can be obtained within 2 hours, the kit is high in specificity, and the sensitivity of the kit can reach 100copies / microliter.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a kit for detecting 10 kinds of respiratory tract infection pathogens and a use method thereof. Background technique [0002] Respiratory tract infection is one of the most common diseases in the world, and its incidence rate plays a major role in the overall structure of the morbidity rate of residents in various countries. About 10% of the residents suffer from respiratory tract infection at the peak of the epidemic each year. Respiratory tract infection can lead to symptoms such as rhinitis, runny nose, nasal congestion, cough, mild pharyngitis, general fever, etc., and severe cases can lead to dyspnea and even death. Acute respiratory infection has become one of the important infectious diseases of children at home and abroad, and its incidence rate ranks first among all kinds of diseases in children. The pathogens that cause respiratory tract infections in children mainly i...

AI Technical Summary

Problems solved by technology

[0005] Although ordinary fluorescent PCR detection reagents have good sensitivity and specificity, most of them are aimed at single or several pathogens. If you want to detect multiple pathogens, you need to use multiple sets of different detection kits, which is inconvenient to operate and expensive; Although the immunoassay detection reagent can detect multiple pathogens at the same time, because the immunoassay detects the specific antibodies produced by the organism caused by the pathogens in the sample, there is a long window period and the sensitivity is relatively low; and for bacteria For the detection of pathogen-like pathogens, although the bacterial culture method has high accuracy, it takes a long time and the culture effect is poor. Many bacteria cannot be cultured, which delays the best time for treatment

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