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Method and special kit for authenticating legionella toxicity

A Legionella and kit technology, applied in the field of identifying the virulence of Legionella, can solve the problems of unconfirmed human-to-human transmission, and achieve the effects of improving measurement accuracy, small sample volume, and low detection cost

Inactive Publication Date: 2014-11-26
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, human-to-human transmission has not been confirmed

Method used

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  • Method and special kit for authenticating legionella toxicity
  • Method and special kit for authenticating legionella toxicity
  • Method and special kit for authenticating legionella toxicity

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0060] Embodiment 1, adopt the method of the present invention to compare the virulence of existing bacterial strain

[0061] Legionella ATCC33152, namely Legionella pneumophila subsp. pneumophila Brenner et al. numbered 33152 in ATCC, is a virulent strain.

[0062] Legionella ATCC33156, ie Legionella pneumophila subsp. fraseri Brenner et al. numbered 33156 in ATCC, is a virulent strain.

[0063] Legionella ATCC43878 is the Legionella brunensis Wilkinson et al. numbered 43878 in ATCC, an attenuated strain.

[0064] Legionella ATCC35249 is the legionella (Legionella spiritensis Brenner et al.) numbered 35249 in ATCC, an attenuated strain.

[0065] Legionella ATCC35252 is the Legionella cherrii Brenner et al numbered 35252 in ATCC, an attenuated strain.

[0066] Legionella ATCC35304 is the Legionella rubrilucens Brenner et al. numbered 35304 in ATCC, an attenuated strain.

[0067] The above 6 strains were respectively used as the strains to be tested as follows:

[0068] 1. ...

Embodiment 2

[0127] Embodiment 2, adopt the method of the present invention to compare the virulence of the bacterial strain collected

[0128] 1. Collection of strains

[0129] In March 2014, water samples were collected from sewers in Beijing.

[0130] The method for isolating Legionella from water samples is as follows:

[0131] 1. Spread the water sample on the GVPC medium plate and place it at 35°C and 2.5% CO 2 Incubate for 48 hours in the incubator.

[0132] 2. After completing step 1, pick the colonies on the plate, use BCYE medium plate, and place it at 35°C, 2.5% CO 2 Cultivate in an incubator, and use the dilution plate separation method to obtain pure strains.

[0133] 3. Carry out morphological identification of each pure strain obtained in step 2, and those that meet the following morphological characteristics are candidate Legionella (Legionella grows slowly and is easily covered by other bacteria, and needs to be observed on a stereoscopic microscope every day): the col...

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Abstract

The invention discloses a method and special kit for authenticating the legionella toxicity. The method comprises the following steps: (1) setting up parameters: Spectral acquisition setup; Grating scan type: Extended; Confocality: Standard; Spectrum Range: Low 100.00; Centre Raman shit / cm<-1>; High 2000.00; Configuration: Laser name 532nm edge; Grating name 18001 / mm; Exposure time / s: 10.00; Accumulations: 1; Objective: 50; Laser power%: 100; (2) detecting; and (3) acquiring a Roman spectrogram. Within an x-coordinate range of 500-2000, if more than one peak with the intensity of 4000 above exists, the to-be-detected legionella is a legionella virulent strain; if the conditions above are not met, the to-be-detected legionella is a legionella attenuated strain. The method disclosed by the invention is rapid, simple and convenient and is low in detection cost.

Description

technical field [0001] The invention relates to a method for identifying the virulence of legionella and a special kit thereof. Background technique [0002] Legionella is an aerobic Gram-negative bacillus, and Legionella pneumophila is the most pathogenic. 48 Legionella species have been found in the genus Legionella, 19 of which are considered to be related to human respiratory tract infections. [0003] Epidemiological investigations have confirmed that Legionella is transmitted to humans through the air through aerosols, and is related to cooling towers, hot water supply systems, steam concentrators, spa spas, humidifiers for clinical respiratory devices, vegetable sprayers in supermarkets, fountains, etc. related. In individual reports, drinking water contaminated by Legionella and contacting soil contaminated by Legionella can also cause Legionnaires' disease. So far, human-to-human transmission has not been confirmed. Legionnaires' disease is characterized by pneu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 刘文军李晶秦天任红宇贾潇潇周海健邵祝军杨利敏
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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