Nanopore three-generation sequencing detection method for plasma virology

A nanopore sequencing and detection method technology, applied in the biological field, can solve the problem of human host nucleic acid pollution target nucleic acid amount and other issues

Active Publication Date: 2020-06-09
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention applies Nanopore third-generation nano-sequencing technology to the detection of blood virus spectrum, which is a brand-new techni

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanopore three-generation sequencing detection method for plasma virology
  • Nanopore three-generation sequencing detection method for plasma virology
  • Nanopore three-generation sequencing detection method for plasma virology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, detection method

[0063] We take 24 asymptomatic adult blood collected in April 2015 at the Beijing Red Cross Blood Center as an example to illustrate the specific implementation of the present invention in sequence.

[0064] 1. Blood sample virus enrichment, nucleic acid extraction and library nucleic acid preparation

[0065] 1) Virus enrichment in plasma samples: Take 6-8ml of whole blood containing EDTA anticoagulant from healthy adults without major clinical symptoms, centrifuge at 3000g for 15min, take the supernatant as plasma, and centrifuge at 10000g for 10min to remove cell debris. Add 0.5-6ml of the supernatant to the ultracentrifuge tube, add Hank's solution to a volume of 12ml, mix carefully, balance with a balance, select a W41 rotor, centrifuge at a speed of 28800g, and centrifuge at 4°C for 2 hours. Remove the supernatant, add Hank's solution to the bottom of the tube to dissolve, and transfer to a 1.5ml centrifuge tube, add Turbo DNase d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a nanopore three-generation sequencing detection method for plasma virology. The method comprises the following steps: preparation of viral nucleic acid in blood samples and incorporation of standard nucleic acid internal reference, construction of nanopore sequencing library, real-time sequencing of nanopore and conversion of real-time fast file, bioinformatics analysis andthe like. The method not only can rapidly detect virus group of a blood sample, but also can improve coverage rate of a whole genome sequence of virus and achieve a purpose of rapidly detecting the virus of the blood sample.

Description

Technical field: [0001] The invention relates to the field of biotechnology, and relates to a method for detecting blood viruses. Background technique: [0002] Blood biosafety is a major livelihood and public security issue, and the supply security of blood resources is the fundamental guarantee for the normal operation of the national medical and health service system. There are increasingly prominent problems such as the contradiction between blood supply and demand and the risk of transfusion-transmitted diseases in my country, and the four routine blood pathogen screenings of HBV, HCV, HIV and TP can no longer meet the safety of clinical blood use. The current research on blood virus spectrum, blood virus Distribution situation There are relatively few domestic and foreign research literatures. Therefore, the study of high-precision detection methods for blood pathogens, especially blood viruses, is an urgent problem to be solved in my country, and it is also a cutting-e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6869C12Q1/6806G16B30/10G16B40/00
CPCC12Q1/6806C12Q1/6869G16B30/10G16B40/00
Inventor 张婷杨帆金奇
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap