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Aspergillus niger strain and use thereof

A strain of Aspergillus niger, the technology of Aspergillus niger, applied in the direction of application, fungi, enzymes, etc., can solve the problems of incomplete enzyme system, high cost, and difficulty in satisfying feed ingredients, etc., to achieve improved digestibility, low production cost, and good economic benefits Effect

Inactive Publication Date: 2009-12-02
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

In developed countries, most of the complex enzyme preparations are prepared by single enzyme compounding method, which has high cost and incomplete enzyme system, and it is difficult to meet the needs of complex feed ingredients.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Obtaining and functional verification of Aspergillus niger strain A46 strain with high yield of compound enzyme for feed

[0021] 1. Acquisition of Aspergillus niger strain A46 strain with high yield of compound enzyme for feed

[0022] 1. Strain mutagenesis

[0023] Aspergillus niger strain (Aspergillus niger) CGMCC 3.316 (purchased from the General Microorganism Center of China Microbiological Culture Collection Management Committee) was inoculated on the potato dextrose medium (PDA) slant, and cultured at 28 ° C for 3 to 4 days until the spores matured. Wash the well-grown spores with an appropriate amount of sterile physiological saline, transfer them into a conical flask with glass beads, and vibrate on a shaker at 28°C for 4-5 hours to break up the spores and activate them. Use four layers of sterile lens paper to filter and remove mycelium fragments, dilute the spore filtrate with an appropriate amount of normal saline, check the spore concentration w...

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PUM

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Abstract

The invention discloses an aspergillus niger strain and use thereof. The aspergillus niger strain is aspergillus niger A46CGMCC No.3084. The strain can produce xylanase, cellulose, pectinase, amylase, beta-mannase and the like with high yield at the same time of xylanase, cellulose, pectinase, amylase, beta-mannase and the like at the same time, as well as an enzyme complex for feeding animals, wherein the optimum pH for active enzymes in the enzyme complex is between 4 and 6 which meets the requirements of the gastrointestinal tracts of the animals. The enzyme complex for feed use can be widely used in livestock feed to reduce anti-nutritional factors, improve the digestibility, create excellent economic benefits and promote ecological environment protection and the sound development of the livestock industry.

Description

technical field [0001] The invention relates to an Aspergillus niger strain and its application, in particular to an Aspergillus niger strain and its application in the preparation of enzyme preparations. Background technique [0002] As a safe and efficient biocatalyst, enzyme preparation can improve the production performance of livestock, poultry and aquatic animals; by eliminating anti-nutritional factors in feed, enzyme preparation can improve the utilization rate of feed raw materials, especially unconventional feed raw materials, and develop new feed resources , has a huge effect on alleviating the shortage of feed resources; at the same time, by improving the digestion and utilization of nutrients, especially nitrogen and phosphorus, enzyme preparations can effectively reduce the environmental pollution of aquaculture caused by the large discharge of certain nutrients in feed. Therefore, feed enzyme preparation has been one of the hot spots of animal nutrition resear...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/42C12N9/26A23K1/165C12R1/685A23K10/14A23K20/189
Inventor 高秀华王海燕张铁涛丁宏标乔宇
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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