The invention belongs to the technical field of
biology, and discloses a controllable multi-channel three-dimensional dynamic culture
system for simulating a
cell mechanical microenvironment, the controllable multi-channel three-dimensional dynamic culture
system comprises a driving platform, a flexible driver and a
cell culture box, the flexible driver is provided with a
planar substrate and an outer wall provided with a periodic protrusion structure, and is provided with a hollow structure serving as an
air chamber; one end is connected with the air path module through an air hole channel, and the other end is sealed; a groove is also formed in the outer surface of the
planar substrate and is used for accommodating cells to be cultured; the flexible driver
air chamber can be pressurized or depressurized, so that the bending degree is enhanced or reduced according to the preset frequency, and a mechanical microenvironment is generated. The three-dimensional dynamic culture
system constructed by the invention can simulate a
cell mechanical microenvironment, realizes dynamic culture of cells, and promotes cell proliferation and expression of specific proteins. In addition, the system can be conveniently designed into multi-channel culture, and has a very good practical application prospect.