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Multi-enzyme complex preparation containing microcapsule and preparation method and application thereof

A technology of microcapsules and complexes, applied in the application and treatment of tobacco, tobacco, etc., can solve the problems of reduced treatment effect and poor effect, and achieve the effects of increased sweetness, improved smoking quality, and coordinated ratio

Active Publication Date: 2013-08-28
YUNNAN REASCEND TOBACCO TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It was found that the compound enzyme, especially the compound enzyme, was used after a period of storage, but the treatment effect was greatly reduced, and the longer the storage time, the worse the effect

Method used

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  • Multi-enzyme complex preparation containing microcapsule and preparation method and application thereof
  • Multi-enzyme complex preparation containing microcapsule and preparation method and application thereof
  • Multi-enzyme complex preparation containing microcapsule and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Prepare the following raw materials by weight percentage: amylase 20.7%, glucoamylase 10.3%, cellulase 8.6%, pectinase 10.9%, catalase 11.4%, laccase 6.4%, microencapsulated protease 9.3%, propylene glycol 7.5% %, 1% citric acid, 0.1% potassium sorbate, and the rest is water. Put amylase, glucoamylase, cellulase, pectinase, catalase, laccase, propylene glycol, citric acid, and potassium sorbate in water, stir to dissolve fully, and form an aqueous solution; then disperse the microencapsulated protease in In the above aqueous solution, a multi-enzyme complex preparation containing microcapsules is obtained.

[0020] The preparation process of microencapsulated protease is as follows: add maltodextrin wall material of 17% of the weight of the protease solution to the flavored protease solution, stir and mix evenly at 100r / min to obtain the wall material film-forming solution; then add the wall material film-forming solution 0.3% by weight of emulsifier distills monoglyce...

Embodiment 2

[0022] Prepare the following raw materials by weight percentage: amylase 22.9%, glucoamylase 11.3%, cellulase 9.8%, pectinase 12.5%, catalase 11.4%, laccase 6.4%, microencapsulated protease 12.5%, polyethylene Glycol 8%, sodium benzoate 0.2%, and the rest is water. Put amylase, glucoamylase, cellulase, pectinase, catalase, laccase, polyethylene glycol, sodium benzoate in water, stir to dissolve fully to form an aqueous solution; then disperse the microencapsulated protease in the above In the aqueous solution, the multi-enzyme complex preparation containing microcapsules can be obtained.

[0023] The preparation process of microencapsulated protease is as follows: add maltodextrin wall material of 23% of the weight of the protease solution to the neutral protease solution, stir and mix at a speed of 500r / min to obtain the wall material film-forming solution; then add the wall material film-forming solution 0.5% by weight of emulsifier distills monoglyceride, stirs and mixes e...

Embodiment 3

[0025] Prepare the following raw materials by weight percentage: amylase 21.5%, glucoamylase 8.4%, cellulase 9.8%, pectinase 11%, catalase 12.5%, laccase 8.1%, microencapsulated protease 8.6%, ethanol 0.2% %, ethyl hydroxybenzoate 0.5%, and the rest is water. Put amylase, glucoamylase, cellulase, pectinase, catalase, laccase, ethanol, and ethyl hydroxybenzoate in water, stir to dissolve fully to form an aqueous solution; then disperse microencapsulated protease in the above In the aqueous solution, the multi-enzyme complex preparation containing microcapsules can be obtained.

[0026] The preparation process of microencapsulated protease is as follows: add maltodextrin wall material of 20% of the weight of the protease solution to the flavored protease solution, stir and mix at a speed of 300r / min to obtain a wall material film-forming solution; then add the wall material film-forming solution 0.3% by weight of emulsifier distills monoglyceride, stirs and mixes evenly at 6000...

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Abstract

Provided are a multi-enzyme complex preparation containing microcapsule and a preparation method and an application thereof. The preparation method includes placing 20.7%-22.9% of amylase, 8.4%-11.3% of saccharifying enzyme, 7.6%-9.8% of cellulose, 10.9%-13.1% of pectinase, 11.4%-15.3% of catalase, 6.4%-9.7% of laccase, 0.2%-8.5% of enzyme stabilizer and 0.01%-0.5% of preservative by weight into water, stirring the mixture to enable the mixture to be dissolved completely and form water solution, dispersing 8.6%-12.5% of microencapsulation protease into the water solution to obtain the multi-enzyme complex preparation containing the microcapsule. The multi-enzyme complex preparation containing the microcapsule is added into tobacco raw materials in a feeding mode according to 0.5%-1% of the weight of the tobacco raw materials in a tobacco threshing re-baking line or a tobacco storage alcoholization or formula tobacco thread making line. The microencapsulation protease in the multi-enzyme complex preparation contains a high polymer material wall material, the protease wrapped by the wall material is not easy to dissolve in the low water solution state and can be dispersed and dissolved quickly in the diluted high water solution state, so that activity loss of the protease and other enzymes caused by the fact that the protease reacts with other enzymes is avoided.

Description

technical field [0001] The invention relates to the development and application of biological enzyme technology, in particular to the preparation of a multi-enzyme complex preparation containing microcapsules and its application in the quality-enhancing treatment of tobacco leaf raw materials. Background technique [0002] Enzymes play a very important role during tobacco leaf curing, aging and storage. The enzyme preparation can degrade excessive starch, protein, cell wall substances and pectin in tobacco leaves, increase aroma and improve smoking quality. In recent years, how to effectively use enzyme preparations to regulate and accelerate the transformation of the internal components of tobacco leaves to aspects that are conducive to improving the quality of cigarettes has become a hot topic of concern to tobacco scientists at home and abroad. [0003] Due to the specificity of the degradation effect of the enzyme, that is, one enzyme can only degrade one type of compon...

Claims

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Application Information

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IPC IPC(8): A24B15/20
Inventor 普元柱王松峰包秀萍邹由黄志强
Owner YUNNAN REASCEND TOBACCO TECH GRP
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