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Preparation method of ABO/RhD blood typing detection reagent card

A technology for detecting reagents and reagent cards, applied in the field of blood transfusion medicine, can solve the problems of wrong ABO blood group, large gaps in the gel, large particles, etc., and achieve the effect of ensuring ionic strength and strong buffering capacity

Active Publication Date: 2010-06-02
JIANGYIN LIBO MEDICINE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At home and abroad, some manufacturers have already produced ABO / RhD blood typing detection reagent cards for ABO blood typing and RhD typing, but the gel they use is dextran gel, and the particle size of this gel is more than 70 nanometers , the particles are larger, and the gaps between the gels are larger, which reduces the sensitivity of ABO blood typing
In addition, the existing cards only perform ABO positive typing and do not perform ABO reverse typing, which reduces the accuracy of ABO blood typing and easily leads to errors in ABO blood typing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042]Step 1, preparation of gel suspension medium

[0043] Described gel suspending medium formula is as follows:

[0044] Methylparaben (5.5-6.5)×10 -4 g / ml

[0045] Propylparaben (1.0-1.5)×10 -4 g / ml

[0046] Glycine (1.6-1.9)×10 -2 g / ml

[0047] Sodium chloride (1.7-1.8)×10 -3 g / ml

[0048] Potassium dihydrogen phosphate (2.1-2.4)×10 -4 g / ml

[0049] Disodium hydrogen phosphate (4.6-4.8)×10 -4 g / ml

[0050] Bovine serum albumin ≤4%,

[0051] Dissolve the above reagents in distilled water and adjust the pH value to 6.6-6.8.

[0052] Step 2. Preparation of the gel

[0053] Polyacrylamide dextran gel is selected, and the particle size is 30-60 nanometers.

[0054] Soak in the gel suspension medium prepared in step 1, then wash with the gel suspension medium for 3-5 times, remove the broken fragments of the gel and the aggregated gel particles, and collect the applicable gelatin powder with uniform particle size and complete spherical shape. gel.

[0055] Step ...

Embodiment 2

[0076] The method of using the ABO / RhD blood typing detection reagent card is as follows:

[0077] 1. The ABO / RhD blood typing test reagent card has six gel tubes. The first tube is anti-A gel, the second tube is anti-B gel, the third tube is anti-D gel, and the fourth to sixth tubes are blank gels (gels containing gel suspension medium). The fourth and fifth tubes are respectively added with the serum (syrup) to be tested and tested with type A and type B reagent red blood cells. The sixth tube is a negative control tube.

[0078] 2. Use LISS solution or physiological saline to prepare the red blood cells of the subject to be tested to a concentration of 0.8-1% (weight percentage), and add them to the first, second, third and sixth microtubes respectively, 50 μl in each tube.

[0079] 3. Adjust known type A and type B erythrocytes with LISS solution or normal saline to a concentration of 0.8-1% (weight percentage), and add them to the fourth and fifth microtubes respectivel...

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PUM

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Abstract

The invention relates to a preparation method of an ABO / RhD blood typing detection reagent card. Six microcolumn gel tubes are arranged on the reagent card and includes a gel tube containing monoclonal anti-A antibodies with IgM property, a gel tube containing monoclonal anti-B antibodies with IgM property, a gel tube containing monoclonal anti-D antibodies with IgM property, two gel tubes which are used for reverse typing and contain a gel suspending medium and a gel tube which is used as a negative control and contains the gel suspending medium. The preparation method comprises the processes of preparing the gel suspending medium, preparing gel, selecting antibodies as well as suspending and packing the gel. The ABO / RhD blood typing detection reagent card prepared by the method has highsensitivity, good specificity and stable quality and can be used for positive typing and reverse typing.

Description

(1) Technical field [0001] The invention relates to a blood type typing card and its application. More specifically, the present invention relates to the composition, preparation method and application of an ABO / RhD blood typing detection reagent card, and relates to the field of blood transfusion medicine. (2) Background technology [0002] Since ABO blood type was discovered in 1900, the importance of blood type identification in blood transfusion therapy was first proposed by Hoktoen (American scientist Heckton) in 1907. In 1908, other scholars proposed the concept of cross-matching experiment, emphasizing the importance of blood type identification. Blood transfusion is one of the important medical methods to save patients' lives in clinic. Correct identification of blood type is the first step in safe blood transfusion. ABO blood type incompatibility will lead to severe immediate hemolytic transfusion reaction in clinical blood transfusion, endangering the life of pati...

Claims

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Application Information

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IPC IPC(8): G01N33/80G01N33/577G01N33/543
Inventor 陈玉平钱国强朱庆平
Owner JIANGYIN LIBO MEDICINE BIOTECH
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