DNA targeted capture array for leukemia chromosome aberration high-throughput sequencing

Abstract

The invention discloses a DNA array for leukemia chromosome aberration high-throughput targeted capture detection. In the DNA array, the contained probe is capable of specific targeted capture of 18 genes involved in 37 chromosome aberrations. A sample and the DNA array are hybridized, chromosome aberration involved gene sequences are captured targetedly, then the captured sequences are subjected to sequencing, the sequencing result is analyzed to screen out the existent chromosome aberration type. The probe contained in the DNA array can specifically bind the 18 genes involved in 37 chromosome aberrations so as to realize rapid screening of leukemia related chromosome translocation. By chip inspection, a plurality of samples can be detected at a time, and the cost is low. For the to-be-detected 37 sites, the accuracy is close to 100%. And cytogenetics professionals are not needed for interpretation of the result.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a DNA target capture array for high-throughput sequencing of leukemia chromosome aberrations. Background technique [0002] With the development of molecular cytogenetics technology, it has been confirmed that the specific changes of certain chromosomes are closely related to the occurrence, development and prognosis of leukemia. The detection of chromosomal abnormalities provides an important basis for the clinical diagnosis, staging, treatment, prognosis of leukemia and the treatment of minimal residual disease. [0003] At present, high-resolution banding technology and fluorescence in situ hybridization (FISH) are used to detect chromosomal aberrations in leukemia at home and abroad. Chromosome banding technology refers to the use of special processing procedures to make certain parts of chromosomes Shows different shades of chromosome banding. These b...

AI Technical Summary

Problems solved by technology

Fluorescence in situ hybridization (FISH) is based on known specific DNA sequences, using fluorescently labeled specific oligonucleotide fragments as probes to hybridize with the DNA molecules of the detected chromosomes, If the target DNA on the detected chromosome is homologous and complementary to the probe used, a hybrid between the target DNA and the probe can be formed, and the hybridization signal is detected by a fluorescent detection system; these have high requirements for technical operation and interpretation Chromosomal fluorescence in situ hybridization probes are expensive and only target a single site; and chromosome technology is only suitable for detecting cells in division phase, with low resolution, and accurate interpretation of results requires long-term professional training

Chromosomal fluorescence in situ hybridization can detect mitotic cells and interphase cells, but it can only detect one abnormality with one known probe each time, with a high rate of missed detection and strict technical requirements. higher cost

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