FISH detection method for aegilops comosa chromosome in wheat

A detection method and chromosome technology, applied in the field of cytogenetics, can solve the problem of no detection and identification method, etc.

Inactive Publication Date: 2016-08-31
CROP RES INST SHANDONG ACAD OF AGRI SCI
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Problems solved by technology

[0008] In order to solve the above problems in the prior art that there is no detection and identification method for the Chromosome of

Method used

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  • FISH detection method for aegilops comosa chromosome in wheat
  • FISH detection method for aegilops comosa chromosome in wheat
  • FISH detection method for aegilops comosa chromosome in wheat

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Embodiment Construction

[0039] In the following examples, unless otherwise specified, the FISH method used is the same as the FISH method described in the content of the invention. The synthesis of the probes was completed by Chengdu Credit Suisse Bio-Co., Ltd., and each probe was labeled with fluorescent colors FAM and Tamra, respectively. That is, the same probe labeled with different fluorescein may produce red or green hybridization signal. Wheat China Spring (CS) was provided by Professor Li Guangrong, School of Life Science and Technology, University of Electronic Science and Technology of China. Miscanthus goatgrass (TA1967) was provided by Dr. Raupp J, Center for Wheat Genomics and Genetic Resources, Kansas State University, USA. Chinese Spring-Gatgrass diploid (XX039) is a cross between Chinese Spring and Goatgrass, hybrid F 1 Obtained by doubling. XX040-XX076 are the backcross progeny material of the cross of XX039 and CS. For the identification of disease resistance of plant materials, ...

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Abstract

The invention belongs to the field of plant cytogenetics, and discloses an in-situ hybridization detection method for identifying and authenticating aegilops comosa M chromosome in wheat background. The detection method comprises the following steps of performing double-color FISH (fluorescence in situ hybridization) on the chromosome preparation of tested material by polynucleotides Oligo-pTa535.1-1 and Oligo-pSc119.2-1, using (GAA)8 as a probe, performing secondary FISH on the chromosome of same cell by an elution double-color FISH signal, and authenticating the aegilops comosa chromosome in the wheat background. The method has the advantages that the aegilops comosa chromosome in a wheat chromosome group is quickly and effectively identified, the application of the polynucleotide probe in triticeae species is widened, a detection method is provided for the authenticating of new variety resource of distant hybridization of wheat-aegilops comosa, and a simple and effective cytogenetic authenticating method is provided for transferring the powdery mildew resistance gene on the aegilops comosa chromosome into the wheat.

Description

technical field [0001] The invention belongs to the field of cytogenetics, and relates to a FISH detection method for rapidly detecting and identifying the chromosomes of Goat grass in wheat. Background technique [0002] Ae. comosa (Ae. comosa, also known as Triticum comosum, 2n=2x=14, genome MM), highly resistant to wheat stripe rust, leaf rust, stem rust, powdery mildew, gramineous cyst nematode and wheat Hessian gall It has salt and alkali tolerance and is an excellent gene source for wheat breeding. In the 1960s, British scientists Riley and others first carried out research work on the transfer of A. chinensis to wheat, and obtained 2M additional lines, 2DL / 2M and 2DS / 2M translocation lines of wheat-C. Sr34 and Yr8 of this chromosome were transferred to wheat (Riley R, Chapman V, Johnson R. Introduction of yellow rustesistance of Aegilops comosa into wheat by genetically induced homoeologous recombination. Nature, 1968, 217(5126):383-384). In 1995, Chinese scientists...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q1/6841C12Q2563/107
Inventor 刘成宫文萍韩冉刘建军宋健民李豪圣刘爱峰曹新有程敦公赵振东
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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