Preparation method and application of three-dimensional graphene electrode for electrochemical biosensor

Abstract

The invention discloses a preparation method and application of a three-dimensional graphene electrode for an electrochemical biosensor. The preparation method comprises the following steps of: fixing spongy graphene in which industrially produced foam nickel is taken as a substrate and which has a three-dimensional structure and is synthesized through chemical vapor deposition on a glass or quartz sheet; connecting the spongy graphene with the three-dimensional structure and a wire by using a silver conductive adhesive; and coating organic silica gel on a connection point of the metal wire and the graphene for insulation to obtain a spongy graphene electrochemical electrode with the three-dimensional structure. The three-dimensional spongy graphene electrode has the outstanding characteristics of high conductivity, high specific surface area, high electrochemical stability and the like, is easily subjected to surface functional modification, and has high detection sensitivity to dopamine and nicotinamide adenine dinucleotide; and a highly sensitive electrochemical biosensor for non-enzymatically and selectively detecting glucose can be obtained after the surface of the electrode is modified by Co3O4.

Description

technical field [0001] The invention relates to a preparation method of a three-dimensional sponge-like graphene electrode for electrochemical biosensing and its application in the field of electrochemical biosensing. Background technique [0002] Electrochemical biosensors play a vital role in today's highly sensitive biomolecular detection, clinical medicine and genetic engineering research fields. Among them, the conductivity, specific surface area and compatibility with biomolecules of the electrochemical electrode are the key factors affecting the detection sensitivity and detection limit of the electrochemical biosensor. Running is critical. However, traditional glassy carbon electrodes have disadvantages such as poor conductivity, difficult surface functional modification, and low specific surface area, which make electrochemical biosensors have low detection sensitivity and poor specific selectivity for biomolecules. Graphene is a new type of carbon nanomaterial th...

AI Technical Summary

Problems solved by technology

However, traditional glassy carbon electrodes have disadvantages such as poor conductivity, difficult surface functional modification and low specific surface area, which make electrochemical biosensors have low sensitivity and poor specific selectivity for biomolecules.

Graphene is a new type of carbon nanomaterial that emerged as a research result of the 2010 Nobel Prize in Physics. It is a two-dimensional honeycomb crystal structure composed of a single layer of carbon atoms, with ultra-high electron mobility, large specific surface area and Excellent physical, chemical, optical and mechanical properties have very broad application prospects in the fields of field effect transistors, nanoelectronic biosensing, transparent conductive films, composite materials, etc. Among them, graphene-modified glassy carbon electrodes have been obtained in the field of biosensing. Extensive research, such as the results published in the invention patent CN 201110112989 showed that the graphene-modified glassy carbon electrode can detect rutin and quercetin at the same time; the results published in the invention patent CN 200910084344.4 proved that the graphene / Nafion film modified glassy Sensitive detection of dopamine, but these modification methods based on graphene oxide are easy to agglomerate graphene, and it is difficult to effectively utilize the characteristics of large specific surface area and high conductivity of graphene

However, there is no report on the use of three-dimensional structure sponge-like graphene to directly prepare electrochemical electrodes and study their application in the field of electrochemical biosensing after functionalization.

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