Medicine-carrying nanometer polymer particle and its prepn and use

A polymer material and drug-loaded nanotechnology, which is applied in the field of new polymer material drug-loaded nanoparticles and their preparation methods and uses, can solve the problems of ineffective reach of target tissues and poor stability of liposome carriers

Inactive Publication Date: 2005-04-27
SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

mPEG-PLGA-mPEG (PELGE) nanoparticles are used as a delivery system. In addition to the general advantages of liposome carriers, they can also overcome the poor stability of liposome carriers. After intravenous injection, they are quickly taken up by the liver and spleen and cannot effectively reach the target tissue. The problem

Method used

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  • Medicine-carrying nanometer polymer particle and its prepn and use
  • Medicine-carrying nanometer polymer particle and its prepn and use
  • Medicine-carrying nanometer polymer particle and its prepn and use

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Accurately weigh 10 mg of PELGE and add it to acetone to form the organic phase. Dissolve 100 μg of a therapeutic gene such as pORF IL-12 plasmid in double distilled water to form an aqueous phase. The plasmid solution was added to the organic phase and homogenized to form colostrum. Weigh Pluronic F68 and add it into double-distilled water to make it fully dissolved, adjust the concentration to 3%, and form the outer water phase. The volume ratio of the inner water phase to the outer water phase is 1:6-1:10. Add colostrum into the external water phase, emulsify with a high-pressure homogenizer, stir the obtained emulsion with magnetic force to evaporate the organic solvent for 4-5 hours, and completely volatilize the acetone to obtain the colloidal solution of PELGE nanoparticles. 3% mannose was added as a scaffold, which was routinely lyophilized for storage.

Embodiment 2

[0047] Accurately weigh 10 mg of PELGE and add it to a mixed organic solvent of dichloromethane (DCM) and acetone (the volume ratio of DCM and AC is 60-100 / 0-40) to form the organic phase. Dissolve 100 μg of a therapeutic gene such as pORF IL-12 plasmid in double distilled water to form an inner water phase. The plasmid solution was added to the organic phase and homogenized to form colostrum. Weigh polyvinyl alcohol (PVA) and add it into double-distilled water to make it fully dissolved, adjust the concentration to 2%, and form the external water phase. Add colostrum into the external water phase, ultrasonically emulsify for 30 seconds, put the obtained emulsion into a beaker, and magnetically stir to evaporate the organic solvent for 3 hours, so that dichloromethane and acetone are completely volatilized, and the colloidal solution of PELGE nanoparticles is obtained. 3% glucose was added as a scaffold, which was routinely lyophilized and stored.

Embodiment 3

[0049] Accurately weigh 20 mg of PELGA and add it into dichloromethane (DCM) to form the organic phase. Dissolve 300 μg of a therapeutic gene such as pORF IL-12 plasmid in double distilled water to form an inner water phase. The plasmid solution was added to the organic phase and homogenized to form colostrum. Weigh polyvinyl alcohol (PVA) and add it into double-distilled water to make it fully dissolved, and adjust its concentration to 1% to form the external water phase. Add colostrum into the external water phase, emulsify with a high-pressure homogenizer for 1 min, and magnetically stir the obtained emulsion to evaporate the organic solvent for 4-5 hours, so that the dichloromethane is completely volatilized, and the colloidal solution of PELGA nanoparticles is obtained. 3% lactose was added as a scaffolding agent and routinely lyophilized for storage.

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Abstract

The present invention discloses one medicinal polymer (PELGE/PELGA) nanometer particle carrier and its preparation process and use. The carrier material is PELGE material of different molecular weights, different LA/GA ratios and different PEG contents, and the nanometer PELGE/PELGA carrier particle is prepared through evaporation process. The said copolymer is self-assembled in water into nanometer particle or micelle, and its hydrophobic PLGA segment coagulates into the core while the hydrophilic polyglycol forms hydrophilic shell. The carrier may be used for the nanometer preparation of plasmid, nucleic acid vaccine, antisense oligodeoxynucleotide or ribozyme for genetic treatment; the nanometer preparation of various chemical medicines; and the nanometer preparation of polypeptide and protein medicines.

Description

technical field [0001] The invention relates to a novel pharmaceutical polymer material drug-loaded nanoparticle, a preparation method and an application. The preparation method of the drug-loaded nanoparticles is an emulsification-evaporation method. In particular, the polymer material PELGE / PELGA is used as the carrier material to wrap therapeutic genes such as pORF IL-12 plasmids or chemical drugs such as mitoxantrone hydrochloride (DHAQ) or doxorubicin (ADR) or peptides, protein drugs such as thymopentin, The PELGE / PELGA nanoparticles are prepared under the action of mechanical stirring or a high-pressure homogenizer. Background technique [0002] General biodegradable polymer nanoparticle carrier materials include natural and synthetic types, serum albumin, hemoglobin, collagen, gelatin, dextran, albumin, chitin and its derivatives, lecithin, cholesterol Natural biodegradable polymer materials such as biocompatibility are good, but the prepara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/30A61K48/00
Inventor 张志荣孙逊段友容
Owner SICHUAN UNIV
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