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Turbidimetric rapid detection kit for myocardial infarction nano-immunoenhancement and use method thereof

A detection kit and immune-enhancing technology, applied in biological testing, material testing products, and analysis by chemical reaction of materials, etc. The effect of wasting reagents

Inactive Publication Date: 2013-07-03
SUZHOU DIAGVITA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing commercial kits prepared by this method have low overall sensitivity and poor stability after opening the bottle.

Method used

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  • Turbidimetric rapid detection kit for myocardial infarction nano-immunoenhancement and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1, detection of troponin-I (Troponin-I)

[0054] Concentration of 0.7% by mass volume percent of sodium chloride as a stabilizer, 3-[N,N-bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid-sodium hydroxide buffer final concentration of 10mmol / L, the coagulant is polyethylene glycol 8000, its mass volume percent final concentration is 2%, and the preservative is sodium azide with a concentration of 0.1%, which is prepared as R1 reagent.

[0055] Latex particles labeled with multiple strains of specific mouse anti-human troponin-I (Troponin-I), sodium chloride with a mass volume percentage concentration of 0.7% as a stabilizer, and a final concentration of 10 mmol / L 3-[N, N-bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid-sodium hydroxide is a buffer solution, and the preservative is sodium azide with a concentration of 0.1%, which is prepared as R2 reagent.

[0056] From the purified natural or recombinant protein troponin-I (Troponin-I), sodium chlo...

Embodiment 3

[0063] Example 3, Detection of Troponin-I (Troponin-I)

[0064] Concentration of 0.8% by mass volume percent of sodium chloride as a stabilizer, 3-[N,N-bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid-sodium hydroxide buffer final concentration of 50mmol / L, the coagulant is polyethylene glycol 8000, its mass volume percent final concentration is 4%, and the preservative is sodium azide with a concentration of 0.1%, which is prepared as R1 reagent.

[0065] Latex particles labeled with multiple strains of specific mouse anti-human troponin-I (Troponin-I), sodium chloride with a mass volume percentage concentration of 0.8% as a stabilizer, and a final concentration of 50 mmol / L 3-[N, N-bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid-sodium hydroxide is a buffer solution, and the preservative is sodium azide with a concentration of 0.1%, which is prepared as R2 reagent.

[0066] From the purified natural or recombinant protein troponin-I (TroponinI), sodium chlor...

Embodiment 4

[0068] Example 4, Detection of D-dimer (D-dimer)

[0069] Concentration of 0.7% by mass volume percent of sodium chloride as a stabilizer, 3-[N,N-bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid-sodium hydroxide buffer final concentration of 20mmol / L, the coagulant is polyethylene glycol 8000, and its mass volume percentage final concentration is 3%, and the preservative is sodium azide with a concentration of 0.1%, which is prepared as R1 reagent.

[0070] By D-dimer (D-dimer) monoclonal antibody latex particles, sodium chloride with a mass volume percentage concentration of 0.7% is used as a stabilizer, and 3-[N,N-bis(2- Hydroxyethyl) amino]-2-hydroxypropanesulfonic acid-sodium hydroxide is the buffer solution, and the preservative is sodium azide with a concentration of 0.1%, which is prepared as R2 reagent.

[0071] From the purified D-dimer (D-dimer), the mass volume percentage concentration is 0.7% sodium chloride as a stabilizer, and the final concentration is ...

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Abstract

The invention provides a turbidimetric rapid detection kit for myocardial infarction nano-immunoenhancement. The turbidimetric rapid detection kit comprises a reaction-detection integrated device, and a detection kit arranged in the reaction device, wherein a reagent R1, a reagent R2 and a calibrator are contained in the detection kit; simultaneously, quantitative detection for five important myocardial infarction-related biomarkers in one sample can be realized, and the five important biomarkers include content of troponin-I, content of D-dimer, content of myoglobin, content of hypersensitive C-reactive protein (hs-CRP) and content of heart-type fatty acid binding protein (FABP); and the turbidimetric rapid detection kit has an important clinical significance in the aspects of early diagnosis and disease course monitoring for myocardial infarction, treatment monitoring for medicines, and the like. The turbidimetric rapid detection kit provided by the invention realizes the integration of the reagents and the reaction device, and is simple, rapid and accurate to operate, high in sensitivity, strong in specificity, and low in detection cost; and the turbidimetric rapid detection kit is a detection / reagent measurement integrated kit suitable for outpatient and emergency treatment / clinical detection, and wide in instrument application range.

Description

technical field [0001] The invention relates to the field of rapid detection of diseases, in particular to a rapid detection kit for myocardial infarction and a use method thereof. Background technique [0002] About 17 million people die from cardiovascular diseases every year in the world, and more than half of them die from acute myocardial infarction. There are about 10 million myocardial infarction patients in the world every year, and about 3 million of them are acute myocardial infarction with ST segment elevation. In the United States, about 900,000 people suffer from acute myocardial infarction (AMI) each year, and about 225,000 of them die before admission, most of which are caused by arrhythmias. More than 2,400 people die from myocardial infarction every day, more than cancer, chronic respiratory disease, diabetes and traffic accidents combined. [0003] After cardiomyocyte injury, the integrity and permeability of the membrane change, the macromolecules in the...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/82
Inventor 王滨吴一凡
Owner SUZHOU DIAGVITA BIOTECH
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