Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Time resolution fluorescence immunoassay analyzer with multi-channel detection function and application thereof

A time-resolved fluorescence and immunoassay technology, applied in the field of food safety testing and immunochromatography, can solve the problems of weak data analysis and processing ability, low degree of automation, single detection, etc., to meet the requirements of real-time rapid quantitative detection, sensitivity and accuracy The effect of high precision and lower inspection cost

Pending Publication Date: 2019-10-15
江苏省苏微微生物研究有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the time-resolved fluorescence immunoassay analyzer commercialized in my country can only perform a single detection (that is, only one target can be detected at a time), resulting in repeated detection, time-consuming, and waste of raw materials; while the time-resolved fluorescence immunoassay analyzer combined with fluorescence Immunochromatography card simultaneously detects two or more targets, which has not been reported so far
In addition, the currently disclosed time-resolved fluorescence immunoassay analyzer needs to reset the parameters for each measurement, the data analysis and processing ability is weak, and the degree of automation is low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Time resolution fluorescence immunoassay analyzer with multi-channel detection function and application thereof
  • Time resolution fluorescence immunoassay analyzer with multi-channel detection function and application thereof
  • Time resolution fluorescence immunoassay analyzer with multi-channel detection function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 A time-resolved fluorescence immunoassay analyzer with multi-channel detection function

[0049] A time-resolved fluorescence immunoassay analyzer with multi-channel detection function, comprising an optical module 1, a mechanical transmission module 2, a data processing module 3, a printer and a printer module 4, a USB and PC interface 5 and a liquid crystal display arranged on a base plate 8 6. The optical module 1, mechanical transmission module 2, printer and printer module 4, USB and PC interface 5, and liquid crystal display 6 are all connected to the data processing module 3 by communication;

[0050] The optical module 1 of the instrument of the present invention is composed of an excitation light path, an emission light path and a photoelectric converter, wherein the excitation light path adopts an ultraviolet LED lamp to emit ultraviolet light, the diaphragm adjusts the intensity of the light beam, and the optical filter selects 340nm excitation light...

Embodiment 2

[0058] Example 2 Preparation and use of parameter chip card

[0059] SW Immunoquantitative Instrument Project Setting Software (Version 1.0.11), developed by Jiangsu Suwei Microbiology Research Co., Ltd. using C language, the size is 876kb, the software language is Simplified Chinese, and the application platform is Windows XP / 7 / 8 / 10 system.

[0060] Standards of deoxynivalenol (DON) and zearalenone (ZEN) were purchased from Sigma, with a purity of ≥99%. Contactless IC card, purchased from a gift card digital store.

[0061] SW-2 time-resolved fluorescence immunoassay analyzer, built-in data sensing read-write module, developed by Jiangsu Suwei Microbial Research Co., Ltd. DON-ZEN dual time-resolved fluorescent immunochromatography card, developed by Jiangsu Suwei Microbial Research Co., Ltd.

[0062] ① Acquisition of multi-item parameters

[0063] Standard working solution detection: Prepare DON and ZEN standard products into mixed standard working solutions with concentra...

Embodiment 3

[0067] Example 3 The time-resolved fluorescence immunoassay analyzer of the present invention, combined with the DON-ZEN dual time-resolved fluorescence immunochromatography card, is used for the rapid quantitative detection of DON and ZEN in wheat and corn

[0068] SW-2 time-resolved fluorescence immunoassay analyzer, built-in data sensing read-write module, externally equipped with a parameter chip card (DON-ZEN) for fluorescence immunochromatography determination, developed by Jiangsu Suwei Microbial Research Co., Ltd.

[0069] DON-ZEN dual time-resolved fluorescent immunochromatography card, developed by Jiangsu Suwei Microbial Research Co., Ltd.

[0070] ①Preparation of tested samples: Accurately weigh 5.0 g each of wheat and corn samples that are uniformly crushed, put them into 50 mL centrifuge tubes, add 25 mL of 60% methanol-water respectively, mix well, seal, and oscillate on a vortex mixer Extract for 10 minutes; centrifuge at 4000 rpm for 5 minutes, and take the su...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a time resolution fluorescence immunoassay analyzer with a multi-channel detection function and an application thereof and belongs to the immunochromatography technology fieldand the food safety detection field. The analyzer includes an optical module, a mechanical transmission module, a data processing module, a printer, a printer module, a USB, a PC interface, and a liquid crystal display which are arranged on a base plate. The optical module, the mechanical transmission module, the printer, the printer module, the USB, the PC interface, and the liquid crystal display are communicated and connected with the data processing module. The analyzer also includes a data induction reading and writing module. The data induction reading and writing module is communicatedand connected with the data processing module. Multi-project parameter setting and reading are realized through the data induction reading and writing module and a parameter chip card so as to possess the multi-channel detection function. More than two mycotoxins in cereals, food and fodders can be simultaneously detected. Operation is simple, sensitivity is high, accuracy is high too, and cost is low, and the analyzer is widely suitable for various occasions and needs of people in different levels.

Description

technical field [0001] The invention relates to a time-resolved fluorescence immunoassay analyzer with multi-channel detection function and its application, belonging to the technical field of immune chromatography and the field of food safety detection. Background technique [0002] Time-resolved fluorescent immunoassay (TRFIA) is a rapid immunochromatographic detection technology emerging in recent years. Its principle is to use special fluorescent lanthanide ions combined with chelating agents as tracers. A reaction occurs in a certain antigen-antibody immune reaction system, and the specific fluorescence intensity in the product is measured with a time-resolved fluorescence immunoassay analyzer, so as to achieve the purpose of quantitative analysis of the analyte. Using the TRFIA method to detect trace substances has the characteristics of simple operation, convenience, and speed, and has the advantages of high sensitivity, stability, and high accuracy. Therefore, it has...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64G01N33/533G01N33/53G01N21/01
CPCG01N33/533G01N33/5302G01N21/6408G01N21/01G01N2021/0112
Inventor 张海涛张一平祁苏娴严艺琳周曦朱亚俊翁原张辰
Owner 江苏省苏微微生物研究有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com