Time resolution fluorescence immunoassay analyzer with multi-channel detection function and application thereof

A time-resolved fluorescence and immunoassay technology, applied in the field of food safety testing and immunochromatography, can solve the problems of weak data analysis and processing ability, low degree of automation, single detection, etc., to meet the requirements of real-time rapid quantitative detection, sensitivity and accuracy The effect of high precision and lower inspection cost

Pending Publication Date: 2019-10-15
江苏省苏微微生物研究有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the time-resolved fluorescence immunoassay analyzer commercialized in my country can only perform a single detection (that is, only one target can be detected at a time), resulting in repeated detection, time-consuming, and waste of raw materials; while the time-resolved fluorescence immunoassay analyzer combined with

Method used

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  • Time resolution fluorescence immunoassay analyzer with multi-channel detection function and application thereof
  • Time resolution fluorescence immunoassay analyzer with multi-channel detection function and application thereof
  • Time resolution fluorescence immunoassay analyzer with multi-channel detection function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0048] Example 1 A time-resolved fluorescence immunoassay analyzer with multi-channel detection function

[0049] A time-resolved fluorescence immunoassay analyzer with multi-channel detection function, comprising an optical module 1, a mechanical transmission module 2, a data processing module 3, a printer and a printer module 4, a USB and PC interface 5, and a liquid crystal display arranged on the bottom plate 8 6; The optical module 1, the mechanical transmission module 2, the printer and the printer module 4, the USB and PC interface 5, and the liquid crystal display 6 are all communicatively connected with the data processing module 3;

[0050] The optical module 1 of the instrument of the present invention is composed of an excitation light path, an emission light path, and a photoelectric converter. The excitation light path uses an ultraviolet LED lamp to emit ultraviolet light, the diaphragm adjusts the intensity of the light beam, and the filter selects 340nm excitation l...

Example Embodiment

[0058] Example 2 Preparation and use of parameter chip card

[0059] SW immune quantitative instrument project setting software (Version 1.0.11), developed in C language by Jiangsu Suwei Microbial Research Co., Ltd., the size is 876kb, the software language is simplified Chinese, and the application platform is Windows XP / 7 / 8 / 10 system.

[0060] Standard products of deoxynivalenol (DON) and zearalenone (ZEN), purchased from Sigma, purity ≥99%. Contactless IC card, purchased from gift card digital franchise store.

[0061] SW-2 time-resolved fluorescence immunoassay analyzer, with built-in data sensing read-write module, developed by Jiangsu Suwei Microbial Research Co., Ltd. DON-ZEN dual time-resolved fluorescence immunochromatography card, developed by Jiangsu Suwei Microbial Research Co., Ltd.

[0062] ① Obtaining multi-project parameters

[0063] Standard working solution detection: DON and ZEN standard products are prepared into mixed standard working solutions with concentrations...

Example Embodiment

[0067] Example 3 The time-resolved fluorescence immunoassay instrument of the present invention, combined with the DON-ZEN dual time-resolved fluorescence immunochromatographic card, is used for the rapid quantitative detection of DON and ZEN in wheat and corn

[0068] SW-2 time-resolved fluorescence immunoassay analyzer, with a built-in data sensing read-write module, and an external fluorescence immunochromatographic measurement parameter chip card (DON-ZEN), developed by Jiangsu Suwei Microbiological Research Co., Ltd.

[0069] DON-ZEN dual time-resolved fluorescence immunochromatography card, developed by Jiangsu Suwei Microbial Research Co., Ltd.

[0070] ①Preparation of the tested sample: accurately weigh 5.0 g each of the crushed wheat and corn samples into a 50 mL centrifuge tube, add 25 mL of 60% methanol-water, mix well, seal, and shake on the vortex mixer Extract for 10 minutes; centrifuge at 4000 rpm for 5 minutes, and take the supernatant as the test sample.

[0071] ②Par...

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Abstract

The invention relates to a time resolution fluorescence immunoassay analyzer with a multi-channel detection function and an application thereof and belongs to the immunochromatography technology fieldand the food safety detection field. The analyzer includes an optical module, a mechanical transmission module, a data processing module, a printer, a printer module, a USB, a PC interface, and a liquid crystal display which are arranged on a base plate. The optical module, the mechanical transmission module, the printer, the printer module, the USB, the PC interface, and the liquid crystal display are communicated and connected with the data processing module. The analyzer also includes a data induction reading and writing module. The data induction reading and writing module is communicatedand connected with the data processing module. Multi-project parameter setting and reading are realized through the data induction reading and writing module and a parameter chip card so as to possess the multi-channel detection function. More than two mycotoxins in cereals, food and fodders can be simultaneously detected. Operation is simple, sensitivity is high, accuracy is high too, and cost is low, and the analyzer is widely suitable for various occasions and needs of people in different levels.

Description

technical field [0001] The invention relates to a time-resolved fluorescence immunoassay analyzer with multi-channel detection function and its application, belonging to the technical field of immune chromatography and the field of food safety detection. Background technique [0002] Time-resolved fluorescent immunoassay (TRFIA) is a rapid immunochromatographic detection technology emerging in recent years. Its principle is to use special fluorescent lanthanide ions combined with chelating agents as tracers. A reaction occurs in a certain antigen-antibody immune reaction system, and the specific fluorescence intensity in the product is measured with a time-resolved fluorescence immunoassay analyzer, so as to achieve the purpose of quantitative analysis of the analyte. Using the TRFIA method to detect trace substances has the characteristics of simple operation, convenience, and speed, and has the advantages of high sensitivity, stability, and high accuracy. Therefore, it has...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/533G01N33/53G01N21/01
CPCG01N21/01G01N21/6408G01N33/5302G01N33/533G01N2021/0112
Inventor 张海涛张一平祁苏娴严艺琳周曦朱亚俊翁原张辰
Owner 江苏省苏微微生物研究有限公司
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