Immune fluorescent test strip component for quickly quantitatively detecting troponin I, detection card component comprising immune fluorescent test strip component and preparation methods for immune fluorescent test strip component and detection card component

Abstract

The invention discloses an immune fluorescent test strip component for quickly quantitatively detecting troponin I, a detection card component comprising the immune fluorescent test strip component and preparation methods for the immune fluorescent test strip component and the detection card component. The test strip component comprises a test strip and an independently packaged platinum porphyrin labelled specific antibody, wherein the test strip comprises a substrate, an absorbent pad, an enveloping analysis film and a sample pad; the enveloping analysis film is provided with a detection line and a quality control line; a specific antibody enveloped by the detection line is a troponin I resistant monoclonal antibody; and a specific antibody enveloped by the quality control line is a rabbit IgG antibody. The detection card component comprises the test strip, a card box consisting of a cover plate and a back plate, and an independently packaged platinum porphyrin labelled specific antibody. An immune fluorescent rapid detection technology is adopted for detecting the level of the troponin I in a human body, the cost is low, the operation is simple, rapid and sensitive, the specificity is high, and the invention has significance for preventing cardiovascular and cerebrovascular diseases.

Description

technical field [0001] The invention belongs to the technical field of immunofluorescence test strips required for medical examination, and in particular relates to an immunofluorescence test strip component for rapid quantitative detection of troponin I and a preparation method thereof, and a detection method supported by the immunofluorescence test strip component Card assembly and method of making same. Background technique [0002] Coronary heart disease has become a major disease affecting the health of the population, and acute myocardial infarction is the main cause of death in coronary heart disease patients. Therefore, how to detect patients with acute myocardial infarction in time and give timely treatment is the key to saving patients' lives. In this regard, the traditional diagnosis is mainly based on typical clinical manifestations, changes in the electrocardiogram, and laboratory enzyme examinations. However, a considerable number of patients with myocardial ...

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Problems solved by technology

[0008] Among them, the Chinese patent application number 200720140929 provides a diagnostic test strip for detecting cardiac troponin I in clinical specimens (whole blood / serum / plasma) by means of rapid immunochromatography, which has certain specificity, sensitivity and It is easy to store, does not require specialized technicians and equipment, and the results are easy to read. However, this patent also cannot meet the needs of short detection time and strong applicability, and has certain defects.

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