Immune fluorescent test strip component for quickly quantitatively detecting troponin I, detection card component comprising immune fluorescent test strip component and preparation methods for immune fluorescent test strip component and detection card component

A technique for quantitative detection of troponin, which is applied to the immunofluorescence test strip assembly for rapid quantitative detection of troponin I and its preparation, the detection card assembly supported by the immunofluorescence test strip assembly and the field of preparation thereof, which can solve the problem of Strong applicability, unable to meet the problems of short detection time, etc., to achieve the effect of removing matrix effect, improving reaction sensitivity and good repeatability

Active Publication Date: 2012-09-12
GUANGZHOU HONGQI OPTICAL INSTR TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Among them, the Chinese patent application number 200720140929 provides a diagnostic test strip for detecting cardiac troponin I in clinical specimens (whole blood/serum/plasma) by means of rapid immunochromatography, which has certain specificit

Method used

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  • Immune fluorescent test strip component for quickly quantitatively detecting troponin I, detection card component comprising immune fluorescent test strip component and preparation methods for immune fluorescent test strip component and detection card component
  • Immune fluorescent test strip component for quickly quantitatively detecting troponin I, detection card component comprising immune fluorescent test strip component and preparation methods for immune fluorescent test strip component and detection card component
  • Immune fluorescent test strip component for quickly quantitatively detecting troponin I, detection card component comprising immune fluorescent test strip component and preparation methods for immune fluorescent test strip component and detection card component

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Experimental program
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Embodiment 1

[0055] as attached figure 1 As shown, an immunofluorescence test strip assembly for rapid quantitative detection of troponin I includes a test strip and a platinum porphyrin-labeled specific antibody that is used in conjunction with the test strip and is independently packaged; wherein the test strip is made of a substrate 1 and the sequentially bonded water-absorbent pad 2, coated analysis membrane 3, and sample pad 6 arranged on the substrate 1; the coated analysis membrane 3 is provided with a detection line 4 and a quality control line 5; the detection line 4. The specific antibody coated is anti-troponin I monoclonal antibody, and the quality control line 5 is coated with specific antibody as anti-rabbit IgG antibody;

[0056] Wherein, the surface of the bottom liner 1 is coated with glue or double-sided tape, which is used to fix the water-absorbing pad 2, the coated analysis film 3 and the sample pad 6, specifically, the coated analysis film 3 is adhered to the bottom ...

Embodiment 2

[0097] The preparation method of the present embodiment is basically the same as that of the first embodiment, the difference is that:

[0098] In step 2, the method for preparing the coating buffer of detection line 4: use 50 mM Tris buffer with pH value of 7.6, which contains 0.8% methanol, 0.6% bovine serum albumin and 1 mg / ml 19C7 antibody.

[0099] The preparation method of the quality control line coating buffer: use 50mM PB buffer solution with a pH value of 7.6, and the PB buffer solution contains 0.7% methanol, 0.5% bovine serum albumin and 0.5mg / ml rabbit IgG antibody.

[0100] Adjust the BIO-DOT film spraying machine so that the film liquid volume is 20ul / 40cm, and put the test strip after spraying the NC film 3 into a vacuum drying oven at 25°C-37°C for drying for 1.5 hours.

Embodiment 3

[0102] The preparation method of the present embodiment is basically the same as that of Example 1, the difference is:

[0103] In step 3, use 0.1M sodium bicarbonate solution to dilute the concentration of anti-troponin I monoclonal antibody and anti-rabbit IgG antibody to 1mg / ml respectively, take 5ml antibody solution respectively, add 40mg fluorescein platinum porphyrin solution respectively and stir well.

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Abstract

The invention discloses an immune fluorescent test strip component for quickly quantitatively detecting troponin I, a detection card component comprising the immune fluorescent test strip component and preparation methods for the immune fluorescent test strip component and the detection card component. The test strip component comprises a test strip and an independently packaged platinum porphyrin labelled specific antibody, wherein the test strip comprises a substrate, an absorbent pad, an enveloping analysis film and a sample pad; the enveloping analysis film is provided with a detection line and a quality control line; a specific antibody enveloped by the detection line is a troponin I resistant monoclonal antibody; and a specific antibody enveloped by the quality control line is a rabbit IgG antibody. The detection card component comprises the test strip, a card box consisting of a cover plate and a back plate, and an independently packaged platinum porphyrin labelled specific antibody. An immune fluorescent rapid detection technology is adopted for detecting the level of the troponin I in a human body, the cost is low, the operation is simple, rapid and sensitive, the specificity is high, and the invention has significance for preventing cardiovascular and cerebrovascular diseases.

Description

technical field [0001] The invention belongs to the technical field of immunofluorescence test strips required for medical examination, and in particular relates to an immunofluorescence test strip component for rapid quantitative detection of troponin I and a preparation method thereof, and a detection method supported by the immunofluorescence test strip component Card assembly and method of making same. Background technique [0002] Coronary heart disease has become a major disease affecting the health of the population, and acute myocardial infarction is the main cause of death in coronary heart disease patients. Therefore, how to detect patients with acute myocardial infarction in time and give timely treatment is the key to saving patients' lives. In this regard, the traditional diagnosis is mainly based on typical clinical manifestations, changes in the electrocardiogram, and laboratory enzyme examinations. However, a considerable number of patients with myocardial ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/52G01N33/68
Inventor 罗琪谢爱武梁万兴李必松
Owner GUANGZHOU HONGQI OPTICAL INSTR TECH
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