Fluorescence dye for detecting fungus and dermatozoon

A fluorescent dyeing and parasite technology, applied in the field of fluorescent dyes, can solve the problems of unstable long-term coexistence, limited clinical application scope, long dyeing time, etc. Effect

Inactive Publication Date: 2017-01-11
付微
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] The purpose of the present invention is to solve the problem that the components of the three dyeing liquids in the current fluorescent staining method cannot coexist stably for a long time, and they need to be prepared and stored separately. The operation is cumbersome, the dyeing time is long, and the specimens to be tested have limitations. Only some specimens with few impurities can be dissolved. , a technical problem with limited scope of clinical application, while providing a fluorescent stain for the detection of fungi and skin parasites

Method used

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  • Fluorescence dye for detecting fungus and dermatozoon
  • Fluorescence dye for detecting fungus and dermatozoon
  • Fluorescence dye for detecting fungus and dermatozoon

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specific Embodiment approach 1

[0054] Embodiment 1: This embodiment is a kind of fluorescent staining agent for detecting fungi and skin parasites, which is specifically composed of water, fluorescent whitening agent, alkali, background counterstaining agent, solubilizing agent and moisturizing agent; The concentration of fluorescent whitening agent in the staining agent is 0.0001g / mL~0.03g / mL, the concentration of alkali is 0.1g / mL~0.2g / mL, and the concentration of background counterstaining agent is 0.0002g / mL~0.03g / mL , the volume concentration of the solubilizing agent is 5%-40%, and the volume concentration of the moisturizing agent is 0.5%-35%.

specific Embodiment approach 2

[0055] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the fluorescent whitening agent is a stilbene type fluorescent whitening agent, preferably a bistriazineamino stilbene type fluorescent whitening agent, Specifically Optical Brightener 28 or Calcofluor Optical Brightener. Others are the same as the first embodiment.

[0056] The structural formula of the fluorescent whitening agent 28 described in the present embodiment is

[0057]

[0058] The CAS number is 4404-43-7 and the molecular formula is C 40 h 44 N 12 o 10 S 2 .

specific Embodiment approach 3

[0059] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the water is double distilled water. Others are the same as the first embodiment.

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Abstract

The invention relates to a fluorescence dye for detecting fungus and dermatozoon. The technical problems that three dyeing liquid ingredients of a current fluorescence dye method cannot stably coexist for a long time, and need to be prepared and stored respectively, operating is tedious, the dyeing time is long, limitation exists in detected samples, some samples with the small impurities can only be dissolved, and the clinical application range is limited are solved. The fluorescence dye is prepared from water, a fluorescent whitening agent, alkali, a background redyeing agent, a chaotropic agent and a moisturizing agent. As the fluorescence dye is a single dosage form, the fluorescent whitening agent, strong alkali and the background redyeing agent can stably coexist; the stability of a product is good, dyeing can be completed only through one step, clinical using is more convenient and fast, original detection which can be completed in 5 minutes or above can be completed in tens of seconds through the fluorescence dye, and the detection efficiency of the fungus and the dermatozoon is greatly improved.

Description

technical field [0001] The invention relates to a fluorescent staining agent for detecting fungi and skin parasites. Background technique [0002] Fungal diseases: Diseases caused by fungi are called mycoses. Clinically, mycosis can be divided into superficial mycosis and deep mycosis or systemic mycosis. Superficial mycoses are caused by fungi that only invade superficial tissues such as human skin, hair, and nails. More than 90% of mycoses in my country are superficial mycoses. Pathogenic fungi that cause systemic mycoses include Histoplasma, Blastomyces, Coccidioides, and Paracoccidioides, which are biphasic fungi that occur in nature as fungal forms such as Cryptococcus, Candida, Aspergillus can cause serious systemic infections. With the development of a large number of organ transplants, the widespread use of immunosuppressants, chemotherapy drugs for tumor patients, and the increase in patients with chronic diseases such as diabetes, the morbidity and mortality of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 付微赵玉宇
Owner 付微
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