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Virus sample preservation solution as well as preparation method and application thereof

A technology for preserving solutions and samples, applied in biochemical equipment and methods, viruses, viruses/phages, etc., can solve problems such as high requirements for environmental equipment, inability to preserve, and live viruses endangering the safety of medical staff and operators, achieving universal The effect of wide sex and safety improvement

Active Publication Date: 2020-09-29
HANGZHOU BIOER TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Ultra-low temperature storage at -80°C requires high environmental equipment. If the laboratory does not have an ultra-low temperature refrigerator, it cannot be stored, and the virus cannot be transported outside the ultra-low temperature environment.
[0007] UTM conventional virus preservation solution preserves the virus and cannot inactivate the virus, and the live virus may endanger the safety of medical staff and operators

Method used

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  • Virus sample preservation solution as well as preparation method and application thereof
  • Virus sample preservation solution as well as preparation method and application thereof
  • Virus sample preservation solution as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0063] The present invention also provides a method for preparing the above-mentioned virus sample preservation solution. The preparation method includes: taking the components of the formula and mixing them evenly to obtain the virus sample preservation solution.

[0064] The preparation method provided by the present invention has simple process and convenient operation, and the virus sample preservation solution provided by the present invention can be prepared by simple mixing, which is suitable for wide popularization and application.

[0065] In addition, based on the virus inactivation effect and the protection effect on viral nucleic acid of the virus sample preservation solution provided by the present invention, the present invention also provides the application of the above virus sample preservation solution in preserving virus samples.

[0066] In some alternative embodiments, the viral sample comprises blood, stool, nasopharyngeal swab, serum, plasma, saliva, asci...

Embodiment 1

[0075] This example provides a virus sample preservation solution, including Tris- and HCl with a final concentration of 50 mmol / L, guanidine isothiocyanate with a mass volume percentage of 5%, and polyethylene glycol with a volume percentage of 5% isopropyl isothiocyanate. Octylphenyl ether, sodium lauroyl sarcosinate with a volume percentage of 5% and EDTA·2Na with a final concentration of 0.1 mmol / L.

Embodiment 2

[0077] This example provides a virus sample preservation solution, including Tris- and HCl with a final concentration of 10 mmol / L, guanidine isothiocyanate with a mass volume percentage of 15%, and polyethylene glycol with a volume percentage of 0.05%. Octylphenyl ether, sodium lauroyl sarcosinate with a volume percentage of 0.05% and EDTA·2Na with a final concentration of 1 mmol / L.

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Abstract

The invention provides a virus sample preservation solution as well as a preparation method and application thereof, and relates to the technical field of biology. The virus sample preservation solution provided by the invention is prepared from a buffer compound with specific concentration, a chaotropic agent, a detergent and a chelating agent. Through the mutual matching of the specific components with the specific concentration, the virus sample preservation solution provided by the invention has the advantages of safety, stability and high efficiency; viruses can be inactivated within 10 minutes; the virus sample nucleic acid can be stably preserved. In addition, the preservation on the virus sample can be realized without ultra-low temperature environment; and the virus sample preservation solution can be applicable to the virus sample collection and transportation in clinic and field environment.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a virus sample preservation solution and a preparation method and application thereof. Background technique [0002] Viruses are special organisms with no cellular structure. Their structure is very simple, consisting of a protein shell and genetic material inside. Viruses cannot survive independently and must live in the cells of other organisms. Once they leave the living cell shell, they do not show any signs of life activity. Viruses are structurally divided into: RNA viruses and DNA viruses. [0003] The detection of viral nucleic acid is suitable for important fields such as early diagnosis of human diseases, auxiliary diagnosis of difficult samples, drug resistance monitoring, disease course monitoring and prediction, guidance of antiviral treatment and efficacy judgment. Therefore, preservation of intact viral nucleic acid is of great significance for downstream gene analy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12Q1/70C12Q1/6806C12R1/93
CPCC12N7/00C12Q1/701C12Q1/6806C12N2730/10111C12N2770/20011C12Q2527/125
Inventor 王虹军金茂峰陈芝娟贺贤汉
Owner HANGZHOU BIOER TECH CO LTD
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