Serum-free medium mesenchymal stem cell inoculation device

Abstract

A serum-free medium mesenchymal stem cell inoculation device, comprising a base, a rotating assembly, a center rod, a supporting rod, a clamping assembly, a test tube, a connecting tube, an arc-shaped air chamber, a fixing rack, a cooling tube and a collecting assembly, wherein the center rod is arranged at the top of the base through the rotating assembly, the supporting rod is annularly equally spaced on an outer wall of the top of the center rod along an axis of the center rod, the test tube is arranged at an end of the supporting rod away from the center rod through the clamping assembly, the connecting tube is arranged on the test tube, the fixing rack is arranged on an outer wall of the bottom of the center rod, the arc-shaped air chamber is in communication with the connecting tube through the cooling tube, and the collecting assembly is arranged on a side of the base. According to the inoculation device, by means of mutual cooperation of the rotating assembly, a moving assembly, the clamping assembly and the collecting assembly, operation errors caused by confusion of diluted test tubes and undiluted test tubes in a liquid adding and pipetting process of a worker are avoided, thereby improving the working efficiency of the worker.

Description

A serum-free culture medium mesenchymal stem cell inoculation device Technical Field

[0001] The invention relates to a serum-free culture medium mesenchymal stem cell inoculation device. Background Art

[0002] Serum-free media and reagents are widely used to culture mammalian and invertebrate cells for the production of monoclonal antibodies, viral antigens, and recombinant proteins. Most serum-free media contain transferrin, which transports ions into cells, and insulin, which regulates glucose uptake. These media also contain proteins such as albumin, fibrin, and fetuin. These proteins perform various functions in cell culture, such as providing a matrix for cell attachment, resisting shear stress in bioreactors, and acting as carriers for lipids and other growth and differentiation factors. Mesenchymal stem cells (MSCs) are multipotent stem cells that possess all the common properties of stem cells, namely, the ability to self-renew and differentiate in multiple directions. They are also the most widely used in clinical practice. Their combination with hematopoietic stem cells can improve transplant success rates and accelerate hematopoietic reconstitution. After high-dose chemotherapy, the co-infusion of MSCs with hematopoietic stem cells significantly accelerates blood cell recovery and is safe and has no adverse effects. MSCs are found not only in the bone marrow but also in skeletal muscle, periosteum, and trabecular bone. Their wide range of tissue types makes them highly valuable for clinical applications.

[0003] Chinese Patent: CN114480126B, a method for culturing mesenchymal stem cells in a serum-free medium and a stem cell inoculation device, comprising: a base, on which a support mechanism is mounted; test tubes, which are detachably mounted on the support mechanism and arranged in a circular pattern around the support mechanism; the method comprises the following steps: treating mesenchymal stem cell tissue with type I collagenase and DNase I; isolating and obtaining a single-cell suspension of the stem cells; by providing a cannula, a test tube, and other structures, when a bacterial solution is dripped into a test tube containing a diluent, squeezing an air bag to pressurize gas into the test tube to achieve aeration, thereby promoting mixing of the bacterial solution and the diluent, thereby improving the dilution effect; due to the provision of a first one-way valve, a second one-way valve, and other structures, the bacterial solution is effectively prevented from being sucked into the cannula, thereby improving reliability.

[0004] This device promotes the mixing of bacterial liquid and diluent, improving the dilution effect. However, when the single-cell suspension needs to be evenly mixed with the diluent, multiple operation steps such as adding liquid, pipetting, and mixing are required. In addition, there are a large number of test tubes, and the staff need to repeat the operation on different test tubes. There are many operation steps. During the process of adding liquid and pipetting, the staff can easily confuse the diluted test tubes with the undiluted test tubes, which can easily lead to operational errors and reduce work efficiency.

[0005] Summary of the Invention

[0006] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the abstract and title of this application to avoid obscuring the purpose of this section, the abstract and the title of the invention, and such simplifications or omissions should not be used to limit the scope of the present invention.

[0007] The technical problem to be solved by the present invention is that when a single-cell suspension needs to be evenly mixed with a diluent, the staff needs to perform multiple operation steps such as adding liquid, pipetting, and mixing. The number of test tubes is large, and the operation needs to be repeated for different test tubes. The numerous steps can easily confuse the diluted test tubes with the undiluted test tubes, leading to operational errors.

[0008] In order to solve the above technical problems, the present invention provides the following technical solutions: a serum-free medium mesenchymal stem cell inoculation device, comprising a base, a rotating assembly, a central rod, a support rod, a clamping assembly, a test tube, a connecting tube, an arc-shaped air chamber, a fixing frame, a cooling tube and a collecting assembly, wherein the central rod is arranged on the top of the base through the rotating assembly to control the rotation of the central rod, the support rod is arranged at equal intervals along the axis of the central rod on the top outer wall of the central rod, the test tube is arranged on the end of the support rod away from the central rod through the clamping assembly to clamp and fix the test tube, the connecting tube is arranged on the test tube, the arc-shaped air chamber is arranged on the top of the base through the fixing frame, the fixing frame is arranged on the outer wall of the bottom of the central rod, the arc-shaped air chamber is connected to the connecting tube through the cooling tube, and the collecting assembly is arranged on one side of the base for collecting the test tube;

[0009] The rotating assembly includes a reset groove, a slide groove, a third connecting rod, a first slider, a first connecting plate and a first pressing block. The reset groove and the slide groove are arranged in a circular and equidistant manner along the axis of the center rod on the bottom inner wall of the center rod. The reset groove is connected to the slide groove. The third connecting rod is arranged inside the center rod. The first slider is arranged on the third connecting rod. The end of the first slider away from the third connecting rod is located in the reset groove. The first connecting plate is arranged at the bottom end of the third connecting rod. The first pressing block is arranged at the end of the first connecting plate away from the third connecting rod. A moving assembly is arranged inside the third connecting rod to assist the rotation of the rotating assembly.

[0010] As a preferred solution of the serum-free medium mesenchymal stem cell inoculation device described in the present invention, the movable component includes a third connecting block, a fourth connecting rod, a third spring, a second connecting plate, a second pressing block and a second slider, the third connecting block and the fourth connecting rod are both arranged inside the third connecting rod, the third connecting block is connected to the fourth connecting rod through the third spring, the second connecting plate is arranged at the bottom end of the fourth connecting rod, the second pressing block is arranged at the end of the second connecting plate away from the fourth connecting rod, the second pressing block is located on one side of the first pressing block, and the second slider passes through the rectangular through hole of the first pressing block and is fixedly connected to the second pressing block.

[0011] As a preferred solution of the serum-free medium mesenchymal stem cell inoculation device described in the present invention, the clamping assembly includes a first connecting block, a first connecting rod, a first spring, two fixed blocks and two fixed clamps, the first connecting block is arranged at the end of the support rod away from the center rod, the first connecting rod is arranged in the first connecting block, the first spring is sleeved on the first connecting rod, the two fixed blocks are symmetrically arranged on both sides of the first connecting block and are rotatably connected to the first connecting rod, the fixed clamp is arranged on the fixed block, and a pulling member is provided on the fixed block, and the fixed clamp is loosened by pulling the fixed block.

[0012] As a preferred solution of the serum-free culture medium mesenchymal stem cell inoculation device described in the present invention, the pulling member includes a second connecting block, a connecting rope, a first fixed plate and a second spring, the second connecting block is arranged inside the center rod, the connecting rope is arranged on the second connecting block, the other end of the connecting rope passes through the inside of the support rod and is fixedly connected to the two fixed blocks, the first fixed plate is slidably arranged inside the support rod, the connecting rope passes through the first fixed plate and is fixedly connected to it, and the second spring is arranged on the side of the first fixed plate close to the second connecting block.

[0013] As a preferred solution of the serum-free medium mesenchymal stem cell inoculation device described in the present invention, the collection component includes a collection box, a baffle, a fifth connecting rod, a second protrusion and a first protrusion, the collection box is arranged on one side of the base, the baffle is equidistantly arranged in the collection box through the fifth connecting rod, the second protrusion is arranged on the fifth connecting rod close to the arc-shaped air chamber, the first protrusion is equidistantly arranged on the arc-shaped air chamber along the axis of the arc-shaped air chamber, and the first protrusion and the second protrusion cooperate with each other.

[0014] As a preferred embodiment of the serum-free medium mesenchymal stem cell inoculation device of the present invention, the bottom end of the third connecting block close to one end of the second connecting block is set as a slope, and the top end of the second connecting block close to one end of the third connecting block is set as a slope, so that the second connecting block can drive the pulling member to release the clamping assembly from fixing the test tube.

[0015] As a preferred embodiment of the serum-free medium mesenchymal stem cell inoculation device of the present invention, a second connecting rod is provided at the top of the central rod, a second fixing plate is provided at the top of the second connecting rod, and the end of the second fixing plate away from the second connecting rod is located at the top of the test tube, so that the second fixing plate assists the cooling tube in separating from the connecting tube.

[0016] The beneficial effects of the present invention are as follows: when a single-cell suspension needs to be evenly mixed with a diluent, the staff needs to perform multiple operation steps of adding liquid, pipetting, and mixing. The number of test tubes is large, and the staff needs to repeat the operation on different test tubes, which involves many operation steps. The present invention cooperates with the rotating component, the moving component, the clamping component, and the collecting component to avoid operational errors caused by the staff confusing the diluted test tubes with the undiluted test tubes during the process of adding liquid and pipetting, thereby improving the work efficiency of the staff. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort. Among them:

[0018] FIG1 is a schematic diagram of the overall structure of a serum-free medium mesenchymal stem cell inoculation device.

[0019] FIG2 is a partial cross-sectional view of a serum-free medium mesenchymal stem cell inoculation device.

[0020] FIG3 is an enlarged view of point A in FIG2 of the serum-free medium mesenchymal stem cell inoculation device.

[0021] FIG4 is an enlarged view of point B in FIG2 of the serum-free medium mesenchymal stem cell inoculation device.

[0022] FIG5 is a schematic diagram showing the position structure of the clamping components of the serum-free medium mesenchymal stem cell inoculation device.

[0023] FIG6 is a schematic diagram showing the position structure of the reset groove and the slide groove of the serum-free medium mesenchymal stem cell inoculation device.

[0024] FIG. 7 is a schematic diagram showing the position structure of the third connecting rod of the serum-free medium mesenchymal stem cell inoculation device.

[0025] FIG8 is a schematic diagram showing the position structure of the first connecting plate of the serum-free medium mesenchymal stem cell inoculation device.

[0026] FIG9 is an enlarged view of point C in FIG8 of the serum-free medium mesenchymal stem cell inoculation device.

[0027] FIG10 is a partial cross-sectional view of the collection box of the serum-free medium mesenchymal stem cell inoculation device.

[0028] FIG11 is a schematic diagram showing the position structure of the fourth connecting rod of the serum-free medium mesenchymal stem cell inoculation device.

[0029] In the figure: 1. base; 2. arc-shaped air chamber; 3. air bag; 4. test tube; 5. cooling tube; 6. fixing clamp; 7. fixing block; 8. first connecting rod; 9. first spring; 10. first connecting block; 11. second connecting rod; 12. reset groove; 13. first slider; 14. center rod; 15. fixing frame; 16. third connecting rod; 17. first connecting plate; 18. support rod; 19. first pressing block; 20. connecting rope; 21. second connecting block; 22. first fixing plate; 23. second spring; 24. third connecting block; 25. fourth connecting rod; 26. second connecting plate; 27. second pressing block; 28. collecting box; 29. ​​baffle; 30. first protrusion; 31. second protrusion; 32. fifth connecting rod; 33. torsion spring; 34. second fixing plate; 35. second slider; 36. slide groove; 37. connecting tube; 38. third spring. DETAILED DESCRIPTION

[0030] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0031] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0032] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive of other embodiments.

[0033] Example 1

[0034] 1 to 6 , which illustrate a first embodiment of the present invention, a serum-free medium mesenchymal stem cell inoculation device is provided.

[0035] Specifically, the base 1, the rotating assembly, the central rod 14, the support rod 18, the clamping assembly, the test tube 4, the connecting tube 37, the arc-shaped air chamber 2, the fixing frame 15, the cooling tube 5 and the collecting assembly, the central rod 14 is arranged at the top of the base 1 through the rotating assembly to control the rotation of the central rod 14, the support rod 18 is arranged equidistantly on the top outer wall of the central rod 14 along the axis of the central rod 14, the test tube 4 is arranged at the end of the support rod 18 away from the central rod 14 through the clamping assembly to clamp and fix the test tube 4, the connecting tube 37 is arranged on the test tube 4, the arc-shaped air chamber 2 is arranged at the top of the base 1 through the fixing frame 15, the fixing frame 15 is arranged on the outer wall of the bottom of the central rod 14, the arc-shaped air chamber 2 is connected to the connecting tube 37 through the cooling tube 5, and the collecting assembly is arranged on one side of the base 1 for collecting the test tube 4;

[0036] The rotating assembly includes a reset groove 12, a slide groove 36, a third connecting rod 16, a first slider 13, a first connecting plate 17 and a first pressing block 19. The reset groove 12 and the slide groove 36 are arranged in a circular and equidistant manner along the axis of the center rod 14 on the bottom inner wall of the center rod 14. The reset groove 12 is connected to the slide groove 36. The third connecting rod 16 is arranged inside the center rod 14. The first slider 13 is arranged on the third connecting rod 16. The end of the first slider 13 away from the third connecting rod 16 is located in the reset groove 12. The first connecting plate 17 is arranged at the bottom end of the third connecting rod 16. The first pressing block 19 is arranged at the end of the first connecting plate 17 away from the third connecting rod 16. A moving part is provided inside the third connecting rod 16 to assist the collection assembly in collecting the test tube 4.

[0037] The base 1 is cylindrical and serves as the mounting base of the device. The center rod 14 is rotatably mounted on the center of the top of the base 1 through a rotating assembly. The rotation of the center rod 14 is controlled by the rotating assembly. The rotating assembly consists of a reset groove 12, a slide groove 36, a third connecting rod 16, a first slider 13, a first connecting plate 17 and a first pressing block 19. The reset groove 12 and the slide groove 36 are arranged on the bottom inner wall of the center rod 14 in a ring-shaped and equidistant manner along the axis of the center rod 14. The reset grooves 12 are connected to each other through the slide groove 36. The slide groove 36 is arc-shaped, and the number of slide grooves 36 corresponds to the number of test tubes 4. One end of the slide groove 36 is connected to the top of the reset groove 12, and the other end is connected to the bottom end of the reset groove 12. The third connecting rod 16 is movably installed inside the center rod 14, and a spring is installed at the bottom of the third connecting rod 16. One end of the first slider 13 is embedded in the inner wall of the third connecting rod 16, and the other end is embedded in the reset groove 12. A first connecting plate 17 is fixedly installed at the bottom end of 16, and a first pressing block 19 is fixedly installed at one end of the first connecting plate 17 away from the third connecting rod 16. A moving part is also installed inside the third connecting rod 16 to assist the collecting assembly in collecting the test tube 4. A plurality of support rods 18 are fixedly installed on the outer surface of the top end of the center rod 14. The support rods 18 are arranged in a circular manner and equidistantly along the axis of the center rod 14. A clamping assembly is installed at one end of the support rod 18 away from the center rod 14 to clamp and fix the test tube 4. A plurality of fixing frames 15 are fixedly installed on the outer surface of the bottom of the center rod 14. The fixing frames 15 are arranged in a circular manner and equidistantly along the axis of the center rod 14. The arc-shaped air chamber 2 is at the top of the base 1 and is fixedly installed on the fixing frame 15. A connecting pipe 37 is installed at the sealing end of the test tube 4. The arc-shaped air chamber 2 is connected to the connecting pipe 37 through the cooling pipe 5. An air bag 3 is installed on the cooling pipe 5. A collecting assembly is installed on one side of the base 1;

[0038] When it is necessary to mix the single-cell suspension and the diluent, the staff first adds the diluent to each test tube 4 respectively, then uses a pipette to absorb the single-cell suspension, and drops the single-cell suspension into the test tube 4 in front of the staff, and squeezes the air bag 3 to mix the single-cell suspension and the diluent evenly. If further dilution is needed, the first pressing block 19 is pressed downward, and the first pressing block 19 drives the third connecting rod 16 to move downward through the first connecting plate 17. The third connecting rod 16 squeezes the spring at the bottom, and the spring generates deformation to store elastic potential energy. At the same time, the movement of the third connecting rod 16 also drives the first slider 13 from the end of the slide groove 36 at the top of the reset groove 12 to the end of the slide groove 36 at the bottom of the reset groove 12, and the slide groove 36 is in an arc shape as a whole, so that the movement of the first slider 13 drives the center rod 14 to rotate accordingly. Since the number of slide grooves 36 corresponds to the number of test tubes 4, when the first slider 13 slides once inside the slide groove 36, the corresponding center rod 14 The support rod 18 on the upper part drives the test tube 4 to move to the next corresponding position, so that the mixed test tube 4 is rotated to the next position, and the test tube 4 to be mixed is rotated in front of the staff for the staff to operate. Thereafter, the staff releases the first pressing block 19, and the spring at the bottom end of the third connecting rod 16 releases elastic potential energy to drive the third connecting rod 16 to move upward, and the third connecting rod 16 drives the first slider 13 to move upward and slide from the bottom end of the reset groove 12 to the top end for easy reuse; if the liquid has been diluted to the required concentration, the moving component on the third connecting rod 16 is pressed, so that the moving component drives the test tube 4 to rotate through the rotating component, and then the moving component is released. The moving component moves upward, so that the clamping component releases the test tube 4 and drops it into the inside of the collecting component, and the collecting component collects it to avoid operational errors caused by the staff confusing the diluted test tube 4 with the undiluted test tube 4 during the process of adding liquid and pipetting, thereby improving the work efficiency of the staff.

[0039] Example 2

[0040] 1 to 11 , there is shown a second embodiment of the present invention, which is based on the previous embodiment.

[0041] Specifically, the moving part includes a third connecting block 24, a fourth connecting rod 25, a third spring 38, a second connecting plate 26, a second pressing block 27 and a second slider 35. The third connecting block 24 and the fourth connecting rod 25 are both arranged inside the third connecting rod 16. The third connecting block 24 is connected to the fourth connecting rod 25 through the third spring 38. The second connecting plate 26 is arranged at the bottom end of the fourth connecting rod 25. The second pressing block 27 is arranged at the end of the second connecting plate 26 away from the fourth connecting rod 25. The second pressing block 27 is located on one side of the first pressing block 19. The second slider 35 passes through the rectangular through hole of the first pressing block 19 and is fixedly connected to the second pressing block 27.

[0042] The third connecting block 24 is laterally slidably installed inside the third connecting rod 16, and the fourth connecting rod 25 is vertically slidably installed inside the third connecting rod 16. One end of the third connecting block 24 is slidably connected to the inner wall of the fourth connecting rod 25, and a third spring 38 is also installed between the third connecting block 24 and the fourth connecting rod 25. A second connecting plate 26 is fixedly installed on the bottom end of the fourth connecting rod 25, and a spring is installed on the bottom of the second connecting plate 26. A second pressing block 27 is fixedly installed on the end of the second connecting plate 26 away from the fourth connecting rod 25. The second pressing block 27 is adjacent to the first pressing block 19. At the same time, a second slider 35 is fixedly installed on the second pressing block 27. A rectangular through hole is opened at a position corresponding to the second slider 35 on the first pressing block 19, and the second slider 35 passes through the rectangular through hole;

[0043] When the liquid in the test tube 4 has been diluted to the required concentration, there is no need to add single-cell suspension to continue mixing. At this time, the staff presses the second pressing block 27, and the second pressing block 27 drives the first pressing block 19 to move downward through the second slider 35. Since the first pressing block 19 is provided with a rectangular through hole for the movement of the second slider 35, and the second slider 35 is located at the bottom of the rectangular through hole in the initial position, when the first pressing block 19 moves downward, it does not drive the second slider 35 to move downward, but when the second slider 35 moves downward, it can drive the first pressing block 19 to move downward. Similarly, the downward movement of the first pressing block 19 drives the center rod 14 to rotate, so that the test tube 4 that has been diluted to the required concentration rotates to the top of the collection assembly; at the same time, the downward movement of the second pressing block 27 drives the fourth connecting rod 25 to move downward through the second connecting plate 26, and the second connecting The plate 26 squeezes the spring at the bottom downward, and the spring deforms to store elastic potential energy. The fourth connecting rod 25 moves downward, driving the third connecting block 24 to move downward, and contacts the pulling piece on the clamping assembly, so that the third connecting block 24 moves toward the fourth connecting rod 25 to squeeze the third spring 38 until the third connecting block 24 is out of contact with the pulling piece. The third connecting block 24 moves in the direction away from the fourth connecting rod 25 under the action of the elastic force of the third spring 38. At this time, the third connecting block 24 is located below the pulling piece; when the staff releases the second pressing block 27, the spring at the bottom of the second connecting plate 26 releases the elastic potential energy, and the second connecting plate 26 moves upward. The second connecting plate 26 drives the third connecting block 24 to move upward through the fourth connecting rod 25, thereby driving the pulling piece to move. The pulling piece loosens the clamping assembly that fixes the test tube 4, and it falls into the interior of the collecting assembly for collection.

[0044] The clamping assembly includes a first connecting block 10, a first connecting rod 8, a first spring 9, two fixed blocks 7 and two fixed clips 6. The first connecting block 10 is arranged at the end of the support rod 18 away from the center rod 14, the first connecting rod 8 is arranged in the first connecting block 10, the first spring 9 is sleeved on the first connecting rod 8, the two fixed blocks 7 are symmetrically arranged on both sides of the first connecting block 10, and are rotatably connected to the first connecting rod 8. The fixed clip 6 is arranged on the fixed block 7, and a pulling member is provided on the fixed block 7. Pulling the fixed block 7 loosens the fixed clip 6.

[0045] The pulling member includes a second connecting block 21, a connecting rope 20, a first fixed plate 22 and a second spring 23. The second connecting block 21 is arranged inside the center rod 14, and the connecting rope 20 is arranged on the second connecting block 21. The other end of the connecting rope 20 passes through the inside of the support rod 18 and is fixedly connected to the two fixed blocks 7. The first fixed plate 22 is slidably arranged inside the support rod 18. The connecting rope 20 passes through the first fixed plate 22 and is fixedly connected to it. The second spring 23 is arranged on the side of the first fixed plate 22 close to the second connecting block 21.

[0046] The first connecting block 10 is fixedly mounted on one end of the support rod 18 away from the center rod 14. The first connecting block 10 is hollow. The first connecting rod 8 is rotatably mounted inside the first connecting block 10. The first spring 9 is mounted on the first connecting rod 8. Two fixed blocks 7 are symmetrically installed on both sides of the first connecting block 10. The two fixed blocks 7 are also located on both sides of the first connecting rod 8 and are rotatably connected to the first connecting rod 8. A fixing clamp 6 is fixedly installed on the two fixing blocks 7. The pulling member mainly consists of a second connecting block 21, a connecting rope 20, a first fixing plate 22 and a second spring 23. The second connecting block 21 is slidably installed on the inside of the center rod 14, and a limiting block is installed in the center rod 14 to limit the second connecting block 21. A connecting rope 20 is installed on the second connecting block 21. The other end of the connecting rope 20 passes through the inside of the support rod 18 and is fixedly connected to the two fixed blocks 7 on the first connecting block 10. The first fixing plate 22 is slidably installed on the inside of the support rod 18. A cavity is opened inside the support rod 18 for the first fixing plate 22 to move. The connecting rope 20 passes through the center of the first fixing plate 22 and is fixedly connected to the first fixing plate 22. A second spring 23 is fixedly installed on the side of the first fixing plate 22 close to the second connecting block 21, and the second spring 23 is sleeved on the connecting rope 20;

[0047] In the initial state, the fixing block 7 is acted upon by the elastic force of the first spring 9 so that the fixing clamp 6 contracts to achieve clamping and fixing of the test tube 4. If the liquid in the test tube 4 has been diluted to the required concentration, the staff presses the second pressing block 27 so that the fourth connecting rod 25 drives the third connecting block 24 to move downward, and the third connecting block 24 contacts the second connecting block 21, continues to slide, and disengages from the second connecting block 21, sliding to the bottom of the second connecting block 21. At this time, the test tube 4 has moved to the top of the collecting assembly, and the staff releases the second pressing block 27. The spring at the bottom of the second connecting plate 26 releases the elastic potential energy, and the second connecting plate 26 moves upward. The second connecting plate 26 drives the third connecting block 24 upward through the fourth connecting rod 25 When the second connecting block 21 stops moving, the second spring 23 releases the elastic potential energy, driving the first fixing plate 22 to move away from the second connecting block 21. The first fixing plate 22 pulls the second connecting block 21 to its original position through the connecting rope 20 for easy reuse.

[0048] The collecting assembly includes a collecting box 28, a baffle 29, a fifth connecting rod 32, a second protrusion 31 and a first protrusion 30. The collecting box 28 is arranged on one side of the base 1. The baffle 29 is equidistantly arranged in the collecting box 28 through the fifth connecting rod 32. The second protrusion 31 is arranged on the fifth connecting rod 32 close to the arc-shaped air chamber 2. The first protrusion 30 is equidistantly arranged on the arc-shaped air chamber 2 along the axis of the arc-shaped air chamber 2. The first protrusion 30 and the second protrusion 31 cooperate with each other.

[0049] A collecting box 28 is fixedly mounted on one side of the base 1, and baffles 29 are equidistantly arranged in the collecting box 28 through a fifth connecting rod 32. The distance between each two baffles 29 is equal to or greater than the distance between the two sides of the test tube 4. The baffles 29 are fixedly connected to the fifth connecting rod 32, and the fifth connecting rod 32 is rotatably connected to the collecting box 28. A torsion spring 33 is mounted on the fifth connecting rod 32, and a second protrusion 31 is fixedly mounted on the fifth connecting rod 32 near the arc-shaped air chamber 2. A first protrusion 30 is equidistantly mounted on the arc-shaped air chamber 2 along the axis of the arc-shaped air chamber 2. When the pulling member releases the clamping assembly from fixing the test tube 4, The test tube 4 falls between the two baffles 29. Since the distance between each two baffles 29 is equal to or greater than the distance between the two sides of the test tube 4, the baffles 29 can block the two sides of the test tube 4, preventing the test tube 4 from falling into the collection box 28. The collection box 28 can better collect the test tubes 4 that have reached the required concentration, thereby distinguishing the diluted test tubes 4 from the undiluted test tubes 4, which is convenient for the staff to operate later.

[0050] After the test tube 4 enters the collecting box 28, if the center rod 14 rotates again, the center rod 14 can also drive the arc-shaped air chamber 2 to rotate through the fixing frame 15, and the arc-shaped air chamber 2 drives the first protrusion 30 to move. When the first protrusion 30 contacts the second protrusion 31, the first protrusion 30 pushes the second protrusion 31 to drive the fifth connecting rod 32 to rotate, and the fifth connecting rod 32 drives the baffle 29 to rotate. Since the baffles 29 are equidistantly distributed on both sides of the collecting box 28, when the baffle 29 close to the side of the base 1 rotates and squeezes the test tube 4 to move, the movement of the test tube 4 will squeeze the baffle 29 on the other side to rotate, pushing the test tube 4 to the side away from the base 1. At this time, the fifth connecting rod 32 is acted upon by the elastic force of the torsion spring 33 to drive the baffle 29 to return to its original position, so as to collect the next test tube 4, thereby achieving the effect of collecting multiple diluted test tubes 4. After the dilutions in all the test tubes 4 are evenly mixed, they are uniformly collected into the culture dish for coating.

[0051] The bottom end of the third connection block 24 close to one end of the second connection block 21 is set as an inclined surface, and the top end of the second connection block 21 close to one end of the third connection block 24 is set as an inclined surface.

[0052] By setting the bottom end of the third connecting block 24 close to the end of the second connecting block 21 as a slope, and the top end of the second connecting block 21 close to the end of the third connecting block 24 as a slope, when the third connecting block 24 moves downward and contacts the second connecting block 21, the two are directly in contact through the slope, so that the second connecting block 21 can better squeeze the third connecting block 24 to slide into the fourth connecting rod 25, so that the third connecting block 24 can better slide to the bottom of the second connecting block 21, so that the third connecting block 24 can drive the second connecting block 21 to move upward, thereby releasing the clamping assembly from fixing the test tube 4.

[0053] The top of the central rod 14 is provided with a second connecting rod 11 , and the top of the second connecting rod 11 is provided with a second fixing plate 34 . An end of the second fixing plate 34 away from the second connecting rod 11 is located at the top of the test tube 4 .

[0054] A second connecting rod 11 is rotatably installed on the top of the center rod 14, and the second connecting rod 11 and the top of the third connecting rod 16 are fixedly connected. A second fixing plate 34 is fixedly installed on the top of the second connecting rod 11, and the end of the second fixing plate 34 away from the second connecting rod 11 is located at the top of the test tube 4. When the pulling member releases the clamping assembly of the test tube 4, if the test tube 4 cannot fall downward, and since the cooling tube 5 and the connecting tube 37 are plug-in connected, the second fixing plate 34 can assist the cooling tube 5 and the connecting tube 37 to disengage, so that the test tube 4 falls and the collection box 28 collects it.

[0055] During use, when it is necessary to mix the single-cell suspension and the diluent, the staff first adds the diluent into each test tube 4 respectively, then uses a pipette to absorb the single-cell suspension, and drips the single-cell suspension into the test tube 4 in front of the staff, and squeezes the air bag 3 to mix the single-cell suspension and the diluent evenly; if it is necessary to continue dilution, the first pressing block 19 is pressed downward, and the first pressing block 19 drives the third connecting rod 16 to move downward through the first connecting plate 17. The third connecting rod 16 squeezes the spring at the bottom, and the spring generates deformation to store elastic potential energy. At the same time, the movement of the third connecting rod 16 also drives the first slider 13 from the slide groove at the top of the reset groove 12 One end of the first slider 13 moves to the end of the slide groove 36 at the bottom of the reset groove 12, and the movement of the first slider 13 drives the center rod 14 to rotate accordingly. The support rod 18 on the corresponding center rod 14 drives the test tube 4 to move to the next corresponding position, so that the mixed test tube 4 is rotated to the next position, and the test tube 4 to be mixed is rotated in front of the staff for the staff to operate. After that, the staff releases the first pressing block 19, and the spring at the bottom end of the third connecting rod 16 releases the elastic potential energy to drive the third connecting rod 16 to move upward. The third connecting rod 16 drives the first slider 13 to move upward and slide from the bottom end of the reset groove 12 to the top end, which is convenient for repeated use.

[0056] If the liquid has been diluted to the required concentration, the staff presses the second pressing block 27 at this time, and the second pressing block 27 drives the first pressing block 19 to move downward through the second slider 35. Similarly, the first pressing block 19 moves downward to drive the center rod 14 to rotate, so that the test tube 4 that has been diluted to the required concentration rotates to the top of the collection box 28; at the same time, the second pressing block 27 moves downward to drive the fourth connecting rod 25 to move downward through the second connecting plate 26, and the second connecting plate 26 squeezes the spring at the bottom downward, the spring generates deformation to store elastic potential energy, the fourth connecting rod 25 moves downward to drive the third connecting block 24 to move downward, the third connecting block 24 contacts the second connecting block 21, the third connecting block 24 moves toward the fourth connecting rod 25 to squeeze the third spring 38, and the third connecting block 24 continues to slide and connect with the second connecting plate The block 21 disengages from contact and slides to the bottom of the second connecting block 21; at this time, the staff releases the second pressing block 27, the spring at the bottom of the second connecting plate 26 releases elastic potential energy, the second connecting plate 26 moves upward, and the second connecting plate 26 drives the third connecting block 24 to move upward through the fourth connecting rod 25, and the third connecting block 24 drives the second connecting block 21 to move upward, and the second connecting block 21 pulls the two fixed blocks 7 on the first connecting block 10 through the connecting rope 20, and the fixed block 7 squeezes the first spring 9 to expand the fixed clip 6 on the fixed block 7, and the test tube 4 falls into the collecting box 28, so that the test tube 4 is collected to avoid operational errors caused by the staff confusing the diluted test tube 4 with the undiluted test tube 4 during the process of adding liquid and pipetting, thereby improving the work efficiency of the staff.

[0057] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A serum-free medium mesenchymal stem cell inoculation device, characterized in that: include, A base (1), a rotating assembly, a central rod (14), a support rod (18), a clamping assembly, a test tube (4), a connecting tube (37), an arc-shaped air chamber (2), a fixing frame (15), a cooling tube (5) and a collecting assembly, wherein the central rod (14) is arranged at the top of the base (1) through the rotating assembly to control the rotation of the central rod, the support rod (18) is arranged at an annular equidistant distance along the axis of the central rod (14) on the top outer wall of the central rod (14), the test tube (4) is arranged at an end of the support rod (18) away from the central rod through the clamping assembly to clamp and fix the test tube, the connecting tube (37) is arranged on the test tube (4), the arc-shaped air chamber (2) is arranged at the top of the base (1) through the fixing frame (15), the fixing frame (15) is arranged on the bottom outer wall of the central rod (14), the arc-shaped air chamber (2) is connected to the connecting tube (37) through the cooling tube (5), and the collecting assembly is arranged on one side of the base (1) for collecting test tubes; The rotating assembly comprises a reset groove (12), a slide groove (36), a third connecting rod (16), a first slider (13), a first connecting plate (17) and a first pressing block (19); the reset groove (12) and the slide groove (36) are arranged in a ring-shaped manner along the axis of the central rod (14) and are equidistantly staggered on the bottom inner wall of the central rod (14); the reset groove (12) is connected to the slide groove (36); the third connecting rod (16) is arranged inside the central rod (14); the first slider (13) is arranged on the third connecting rod (16); one end of the first slider (13) away from the third connecting rod is located in the reset groove (12); the first connecting plate (17) is arranged at the bottom end of the third connecting rod (16); the first pressing block (19) is arranged at one end of the first connecting plate (17) away from the third connecting rod; and a moving assembly is arranged inside the third connecting rod (16) to assist the collecting assembly in collecting the test tubes.

2. The serum-free medium mesenchymal stem cell inoculation device according to claim 1, characterized in that: The moving assembly comprises a third connecting block (24), a fourth connecting rod (25), a third spring (38), a second connecting plate (26), a second pressing block (27) and a second sliding block (35); the third connecting block (24) and the fourth connecting rod (25) are both arranged inside the third connecting rod (16); the third connecting block (24) is connected to the fourth connecting rod (25) via the third spring (38); the second connecting plate (26) is arranged at the bottom end of the fourth connecting rod (25); the second pressing block (27) is arranged at an end of the second connecting plate (26) away from the fourth connecting rod; the second pressing block (27) is located on one side of the first pressing block (19); and the second sliding block (35) passes through a rectangular through hole of the first pressing block (19) and is fixedly connected to the second pressing block (27).

3. The serum-free medium mesenchymal stem cell inoculation device according to claim 2, characterized in that: The clamping assembly comprises a first connecting block (10), a first connecting rod (8), a first spring (9), two fixed blocks (7) and two fixed clamps (6); the first connecting block (10) is arranged at one end of the support rod (18) away from the center rod; the first connecting rod (8) is arranged in the first connecting block (10); the first spring (9) is sleeved on the first connecting rod (8); the two fixed blocks (7) are symmetrically arranged on both sides of the first connecting block (10) and are rotatably connected to the first connecting rod (8); the fixed clamp (6) is arranged on the fixed block (7); a pulling member is arranged on the fixed block (7); the fixed clamp is loosened by pulling the fixed block.

4. The serum-free medium mesenchymal stem cell inoculation device according to claim 3, characterized in that: The pulling member comprises a second connecting block (21), a connecting rope (20), a first fixing plate (22) and a second spring (23); the second connecting block (21) is arranged inside the center rod (14); the connecting rope (20) is arranged on the second connecting block (21); the other end of the connecting rope (20) passes through the inside of the support rod (18) and is fixedly connected to the two fixing blocks (7); the first fixing plate (22) is slidably arranged inside the support rod (18); the connecting rope (20) passes through the first fixing plate (22) and is fixedly connected thereto; and the second spring (23) is arranged on a side of the first fixing plate (22) close to the second connecting block (21).

5. The serum-free medium mesenchymal stem cell inoculation device according to claim 1, characterized in that: The collecting assembly comprises a collecting box (28), a baffle (29), a fifth connecting rod (32), a second protrusion (31) and a first protrusion (30); the collecting box (28) is arranged on one side of the base (1); the baffle (29) is equidistantly arranged in the collecting box (28) via the fifth connecting rod (32); the second protrusion (31) is arranged on the fifth connecting rod (32) close to the arc-shaped air chamber; the first protrusion (30) is equidistantly arranged on the arc-shaped air chamber (2) along the axis of the arc-shaped air chamber (2); the first protrusion (30) and the second protrusion (31) cooperate with each other.

6. The serum-free medium mesenchymal stem cell inoculation device according to claim 4, characterized in that: The bottom end of the third connection block (24) close to one end of the second connection block (21) is arranged as an inclined surface, and the top end of the second connection block (21) close to one end of the third connection block (24) is arranged as an inclined surface.

7. The serum-free medium mesenchymal stem cell inoculation device according to claim 1, characterized in that: A second connecting rod (11) is arranged at the top end of the central rod (14), a second fixing plate (34) is arranged at the top end of the second connecting rod (11), and an end of the second fixing plate (34) away from the second connecting rod is located at the top end of the test tube (4).

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