The invention belongs to the technical field of analysis detection, and relates to a preparation method of a gold nanoparticle-doped molecular imprinting electrochemistry sensor for dopamine detection. The method comprises gold electrode pretreatment, gold nanoparticle self-assembly modification electrode preparation, molecular imprinting self-assembly solution preparation, electropolymerization reaction, template molecule elution and other steps so as to prepare the molecular imprinting membrane electrochemistry sensor (MIES). The method has advantages of integration of the electrochemistry sensor and the gold nanoparticle-doped imprinted polymer, can be provided for constructing the gold nanoparticle-doped molecular imprinting electrochemistry sensor for dopamine detection so as to improve selectivity of the electrochemistry sensor, provide characteristics of rapid response, good stability and good tolerance, and achieve efficient, sensitive and real-time detection of dopamine in a biological sample, and provides important significance for clinical diagnosis, pathology research, advances in biochemistry, neurochemistry development and the like.