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Preparation method of a gold nanoparticle-doped molecularly imprinted electrochemical sensor for the detection of dopamine

A technology of gold nanoparticles and molecular imprinting, which is applied in the field of analysis and detection, can solve the problems affecting the sensitivity of sensors, etc., and achieve the effect of improving detection sensitivity, increasing sensitivity and fast response

Inactive Publication Date: 2015-09-30
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the molecularly imprinted layers electropolymerized onto the electrode are non-conductive or weakly conductive substances, which greatly affect the sensitivity of the sensor.

Method used

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  • Preparation method of a gold nanoparticle-doped molecularly imprinted electrochemical sensor for the detection of dopamine
  • Preparation method of a gold nanoparticle-doped molecularly imprinted electrochemical sensor for the detection of dopamine
  • Preparation method of a gold nanoparticle-doped molecularly imprinted electrochemical sensor for the detection of dopamine

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Effect test

Embodiment 1

[0033] A method for preparing a gold nanoparticle-doped molecularly imprinted electrochemical sensor for detecting dopamine proposed by the present invention comprises the following specific steps:

[0034] Step 1, gold electrode pretreatment: put the gold electrode (Φ=2mm) through 0.05μm A1 2 o 3 After the suspension was polished, it was rinsed with double distilled water, then ultrasonically cleaned with absolute ethanol and deionized water for 1 min, and then scanned in sulfuric acid solution by cyclic voltammetry (CV) until the pattern was stable, and then double distilled Rinse with water and dry with nitrogen;

[0035] Step 2, preparation of gold nanoparticle self-assembly modified electrode: immerse the gold electrode pretreated in step 1 into a 5 mmol / L ethanol solution containing aminothiophenol (pATP), perform self-assembly for 10 hours, rinse and dry; Then immerse it in 0.5 mg / mL gold nano sol, wash and dry after immersing for 2 hours, and obtain a gold nano self...

Embodiment 2

[0040] A method for preparing a gold nanoparticle-doped molecularly imprinted electrochemical sensor for detecting dopamine proposed by the present invention comprises the following specific steps:

[0041] Step 1, gold electrode pretreatment: put the gold electrode (Φ=2mm) through 0.05μm A1 2 o 3 After the suspension was polished, it was rinsed with double distilled water, then ultrasonically cleaned with absolute ethanol and deionized water for 1 min, and then scanned in sulfuric acid solution by cyclic voltammetry (CV) until the pattern was stable, and then double distilled Rinse with water and dry with nitrogen;

[0042] Step 2, preparation of gold nanoparticle self-assembly modified electrode: immerse the gold electrode pretreated in step 1 into a 10 mmol / L ethanol solution containing aminothiophenol (pATP), perform self-assembly for 15 hours, rinse and dry; Then immerse it into 0.6mg / mL gold nano sol, wash and dry it after immersion for 8 hours, and obtain the gold na...

Embodiment 3

[0047] A method for preparing a gold nanoparticle-doped molecularly imprinted electrochemical sensor for detecting dopamine proposed by the present invention comprises the following specific steps:

[0048] Step 1, gold electrode pretreatment: put the gold electrode (Φ=2mm) through 0.05μm A1 2 o 3 After the suspension was polished, it was rinsed with double distilled water, then ultrasonically cleaned with absolute ethanol and deionized water for 1 min, and then scanned in sulfuric acid solution by cyclic voltammetry (CV) until the pattern was stable, and then double distilled Rinse with water and dry with nitrogen;

[0049] Step 2, preparation of gold nanoparticle self-assembly modified electrode: immerse the gold electrode pretreated in step 1 into 20mmol / L ethanol solution containing aminothiophenol (pATP), perform self-assembly for 20h, rinse and dry; Then immerse it into 0.8 mg / mL gold nano sol, wash and dry after immersion for 15 hours, and obtain gold nano self-assem...

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Abstract

The invention belongs to the technical field of analysis detection, and relates to a preparation method of a gold nanoparticle-doped molecular imprinting electrochemistry sensor for dopamine detection. The method comprises gold electrode pretreatment, gold nanoparticle self-assembly modification electrode preparation, molecular imprinting self-assembly solution preparation, electropolymerization reaction, template molecule elution and other steps so as to prepare the molecular imprinting membrane electrochemistry sensor (MIES). The method has advantages of integration of the electrochemistry sensor and the gold nanoparticle-doped imprinted polymer, can be provided for constructing the gold nanoparticle-doped molecular imprinting electrochemistry sensor for dopamine detection so as to improve selectivity of the electrochemistry sensor, provide characteristics of rapid response, good stability and good tolerance, and achieve efficient, sensitive and real-time detection of dopamine in a biological sample, and provides important significance for clinical diagnosis, pathology research, advances in biochemistry, neurochemistry development and the like.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a preparation method of a molecularly imprinted electrochemical sensor doped with gold nanoparticles for detecting dopamine. Background technique [0002] Dopamine (DA) is an important neurotransmitter in the hypothalamus and pituitary gland, a chemical used to help cells transmit bioelectrical signals, and plays an important role in the central nervous, renal and endocrine systems. Dysregulation of dopamine neurons in the brain is an important cause of conditions such as Parkinson's disease, Huntington's disease and schizophrenia. Therefore, the research on the determination method of dopamine is of great significance to explore its physiological activity and the diagnosis of related diseases. Existing methods for detecting dopamine mainly include spectrophotometry, microdialysis, ion chromatography, chemiluminescence, high performance liquid chromat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/416G01N27/333
Inventor 周学敏姜慧君薛诚韩青文婷婷汪洋王若禹
Owner NANJING MEDICAL UNIV
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