Neural signal recording method and system integrating in-vivo patch clamp and optical fiber records

Abstract

The invention provides a neural signal recording method and a system integrating an in-vivo patch clamp and optical fiber records. The neural signal recording system comprises a fluorescence signal recording unit, an excitation unit, a patch clamp recording unit and a control and processing unit. The patch clamp recording unit comprises an electrophysiological digital-to-analog converter. The fluorescence signal recording unit records fluorescence signals. The patch clamp recording unit records electroneurographic signals. The fluorescence signals and the electroneurographic signals are synchronized in the electrophysiological digital-to-analog converter. The system provided by the invention can simultaneously reflect the change of membrane potential such as pulse discharge and the change condition of neurochemical signals of a related loop, and qualitatively improves dimensionality and form of neural signal records as well as richness of acquired data.

Description

technical field [0001] The invention relates to the technical field of nerve signal recording, in particular to a nerve signal recording method and system capable of simultaneously recording fluorescent signals and electrical signals in combination with in vivo patch clamp and optical fiber recording. Background technique [0002] The study of the nervous system of awake animals is an important topic in contemporary neuroscience research. With the deepening of neuroscience research, the ways and methods of neural signal recording are also undergoing continuous innovation. At present, the recording methods of neural signals are mainly based on fluorescent signal recording and electrical signal recording. In existing experimental techniques, a specific in vivo experiment on awake animals generally adopts a single recording method, that is, only fluorescent signals or electrical signals are recorded. [0003] In the prior art, when the optical fiber recording system is used t...

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Problems solved by technology

[0006] Aiming at the problems existing in the prior art, the present invention provides a nerve signal recording method and system combining in vivo patch clamp and optical fiber recording, and integrates the fluorescent signal recording method and the patch clamp recording method in a set of systems by optimizing design , to solve the problem of monotonous recording methods in vivo experiments

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