Combined detection method for pregnancy folate metabolism, calcium metabolism and H-type hypertension

A technology for folic acid metabolism and combined detection, which is used in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., and can solve problems such as multiple PCR-LDR techniques that have not been reported

Pending Publication Date: 2019-09-20
上海联吉医学检验所有限公司
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Problems solved by technology

[0012] At present, there is no report that the multiplex PCR-LDR technology is specifically used for the joint detection of mutation sites in folic acid metabolism, calcium metabolism in pregnancy, and H-type hypertension medication.

Method used

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  • Combined detection method for pregnancy folate metabolism, calcium metabolism and H-type hypertension
  • Combined detection method for pregnancy folate metabolism, calcium metabolism and H-type hypertension
  • Combined detection method for pregnancy folate metabolism, calcium metabolism and H-type hypertension

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Embodiment 1

[0069] A combined detection method of folic acid metabolism, calcium metabolism and H-type hypertension during pregnancy of the present invention uses bioinformatics knowledge and related bioinformatics software to detect multiple SNP sites (rs1051266, rs1544410) that can be retrieved in public databases , rs1801131, rs1801133, rs2234693, rs9340799, rs731236, rs7975232, rs1801394) sequence information for homology analysis, surrounding SNP analysis, frequency analysis, base content analysis and SNP surrounding sequence analysis, design a specific multiplex PCR primer system and Multiple LDR probe system and optimization. The method employed consists of the following steps:

[0070] Step.1 Obtaining the genomic DNA of the sample: extract the genomic DNA of the whole blood sample with the Qiagen DNA extraction kit;

[0071] Step.2 Reagent storage: Store the received kit in a -20°C environment to avoid repeated freezing and thawing; Step.3 PCR amplification: Take 5.5ul of PCR Mi...

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Abstract

The invention relates to a combined detection method for pregnancy folate metabolism, calcium metabolism and H-type hypertension. The method is characterized in that bioinformatics knowledge and related bioinformatics software are utilized to perform homology analysis, peripheral SNP analysis, frequency analysis, base content analysis and SNP peripheral sequence analysis on the sequence information of multiple SNP loci (rs10512366, rs1544410, rs1801131, rs1801133, rs2234693, rs9340799, rs731236, rs7975232 and rs1801394) which can retrieved in an open database, and then a specific multiple PCR primer system and a multiple LDR probe system are designed and optimized. The method has the advantages that fragment length analysis is performed by combining the multiple PCR-LDR technology and the capillary electrophoresis technology of a genetic analyzer to achieve SNP detection typing, and the method is large in flux, high in specificity and flexibility, stable in result, good in repeatability and fast in detection.

Description

technical field [0001] The invention relates to the technical field of molecular biology genes, and relates to a method for combined detection of folic acid metabolism, calcium metabolism and H-type hypertension during pregnancy. Background technique [0002] (1) Folic acid metabolism during pregnancy [0003] Significance of gene detection of folic acid metabolism ability in pregnant women: folic acid deficiency in early pregnancy is the main cause of fetal neural tube defects. Folic acid deficiency can cause neural tube failure to close, leading to neural tube defects such as spina bifida and anencephaly. Supplementing folic acid for women of childbearing age can effectively prevent neural tube defects. Due to individual genetic differences, there are significant differences in the metabolism of folic acid among individuals, resulting in significant differences in the utilization of folic acid among individuals. [0004] A uniform dose of 0.4mg / d folic acid supplementatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/686C12N15/11
CPCC12Q1/6883C12Q1/686C12Q2600/156C12Q2537/143C12Q2565/125
Inventor 裘敏燕
Owner 上海联吉医学检验所有限公司
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