Application, detection method and kit of MTHFR (Methylene Tetrahydrofolate Reductase) gene SNP (Single Nucleotide Polymorphism) guide folic acid and related nutrient element intake

A technology of nutritional elements and detection methods, applied in the field of medicine, to prevent physical defects and reduce medical costs

Inactive Publication Date: 2010-10-20
吴奇涵 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the effect of folic acid on the occurrence of NTDs may be through genetic metabolic pathways rather than insufficient intake, so there are still 30%-45% of cases that cannot be prevented by implementing this measure

Method used

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  • Application, detection method and kit of MTHFR (Methylene Tetrahydrofolate Reductase) gene SNP (Single Nucleotide Polymorphism) guide folic acid and related nutrient element intake
  • Application, detection method and kit of MTHFR (Methylene Tetrahydrofolate Reductase) gene SNP (Single Nucleotide Polymorphism) guide folic acid and related nutrient element intake
  • Application, detection method and kit of MTHFR (Methylene Tetrahydrofolate Reductase) gene SNP (Single Nucleotide Polymorphism) guide folic acid and related nutrient element intake

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Embodiment Construction

[0049] The method for detecting the SNP genotype of the MTHFR gene that guides the intake of folic acid and related nutrients will be further described below.

[0050] 1 Reagents, materials and instruments

[0051] 1.1 Main reagents and enzymes

[0052] ACD anticoagulant, proteinase K, TaqDNA polymerase, aliquoted dNTPs, PCRMarker,

[0053] Primer 1, MTHFR-677-5': 5'-TGAAGGAGAAGGTGTCTGCGGGA-3'

[0054] Primer 2, MTHFR-677-3': 5'-AGGACGGTGCGGTGAGAGTG-3'

[0055] Primer 3, MTHFR-1298-5': 5'-CTTGGGGAGCTGAAGGACTAC-3'

[0056] Primer 4, MTHFR-1298-3': 5'-CACTTGTGACCATCCGGTTG-3'

[0057] Gel Purification Kit,

[0058] Other reagents: agarose, EB, phenol blue, xylene cyanol, balanced phenol, chloroform, isoamyl alcohol, glacial acetic acid, absolute ethanol, Tris, EDTANa2, SDS, etc.

[0059] 1.2 Main instruments and equipment

[0060] GeneAmp9600 PCR instrument

[0061] EPPENDORF5415C Desktop High Speed ​​Centrifuge

[0062] HITACHIHimacCR21 high-speed refrigerated centrifuge ...

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Abstract

The invention discloses application, a detection method and a kit of SNP of an METHFR gene guiding folic acid and related nutrient element intake. Because of providing different positive locuses of SNP gene types of folic acid metabolic capacity and related nutrient element intake, the reason generating impediment thereof is found, and the guidance for guiding individuals, especially pre and post natal women, to take folic acid and intake related nutrients is provided.

Description

technical field [0001] The invention relates to the use of the SNP (polymorphic site) genotype of the MTHFR (methylenetetrahydrofolate reductase) gene to guide the intake of folic acid and related nutritional elements, and a method for detecting the SNP genotype of the MTHFR gene A test kit and a test kit belong to the technical field of medicine. Background technique [0002] The relationship between human genetics and nutritional metabolism is closely related, and genetics will hardly change in a person's life, which means that most of our physiological process characteristics are determined from the beginning of the formation of fertilized eggs, such as our appearance, height, and personality. Compared with genes, health status is constantly changing. Analysis of genetic genes can help us predict the direction of health development, provide personalized health consultation, and improve the quality of life. [0003] Human beings ingest various foods from the outside worl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 吴奇涵钟鸣窦同海
Owner 吴奇涵
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