Analytical instrument having improved arrangement of reagent section and analytical method
a technology which is applied in the field of analytical instruments having improved arrangement of reagent section and analytical method, can solve the problems of reducing the ratio of glucose which can be transferred from blood cells to blood serum (blood plasma) within the measurement time, affecting the effect of the above-mentioned low-value problem, and reducing the influence of blood cell concentration
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example 1
[0098]In this example, in the measurement of a blood glucose level using a glucose sensor for colorimetry, the influence of the blood cell concentration (hematocrit(Hct)) in blood on the measurement result was examined. As the glucose sensor, sensors (1)-(3) having the structures described below were used. The influence of an Hct on the measurement result was examined based on the time course of absorbance and the bias after the lapse of a predetermined time period.
(Basic Structure of Glucose Sensor)
[0099]The basic structure of the glucose sensors (1)-(3) (excluding reagent portions) is shown in FIG. 14. Specifically, each of the glucose sensors (1)-(3) included a transparent substrate 2A, a transparent cover 4A bonded to the substrate via a spacer 3A, and a capillary 5A defined by the elements 2A-4A. As described in FIG. 14, the dimensions of the capillary 5A were 1.3 mm×9 mm×50 μm. The transparent substrate 2A and the transparent cover 4A were made of a PET material having a thick...
example 2
[0114]In this example, the time course of absorbance of each of the glucose sensors (1)-(3), which were prepared similarly to Example 1, was measured using five kinds of analytes having different glucose levels (Omg / dL, 113 mg / dL, 212 mg / dL, 430 mg / dL and 598 mg / dL). FIGS. 18A-18C show the results.
[0115]As will be understood from FIGS. 18A-18C, in the glucose sensors (1), (2) (inventive glucose sensors), the initial rise of absorbance is sharp and the time until the absorbance becomes close to a constant value is short for any of the analytes of different glucose levels, although the content of enzyme (activity reference) in the reagent portion of the glucose sensors (1), (2) was lower than that in the glucose sensor (3) (comparative glucose sensor). This also means that, as compared with the glucose sensor (3), the relationship between the absorbance and the glucose level in the glucose sensors (1), (2) has high linearity even when the measurement time is relatively short (e.g. wit...
example 3
[0116]The measurement reproducibility was evaluated based on the data obtained in Example 2. Specifically, the measurement reproducibility of each of the glucose sensors (1)-(3) was evaluated by computing the variations in the absorbance five seconds after the start of the measurement with respect to each of the analytes of different glucose levels. The computation results are given in Tables 8-10 below.
TABLE 8Glucose Sensor (1): Inventive Glucose SensorGlucose Level2125980 mg / dL113 mg / dLmg / dL430 mg / dLmg / dLAbsorbance0.0510.1400.2280.3950.5000.0470.1410.2220.3860.4920.0510.1410.2240.3880.5580.0530.1360.2370.3970.5130.0560.1430.2090.3850.475Average0.0520.1400.2240.3900.512S.D.0.000.000.010.000.03C.V.5.7%1.8%4.0%1.2%5.5%
TABLE 9Glucose Sensor (2): Inventive Glucose SensorGlucose Level2125980 mg / dL113 mg / dLmg / dL430 mg / dLmg / dLAbsorbance0.0410.1740.2540.4510.5750.0420.1740.2670.4570.5540.0360.1700.2710.4680.5570.0390.1740.2760.4380.5460.0460.1650.2700.4550.535Average0.0410.1720.2680.4540.5...
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