Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Analytical instrument having improved arrangement of reagent section and analytical method

a technology which is applied in the field of analytical instruments having improved arrangement of reagent section and analytical method, can solve the problems of reducing the ratio of glucose which can be transferred from blood cells to blood serum (blood plasma) within the measurement time, affecting the effect of the above-mentioned low-value problem, and reducing the influence of blood cell concentration

Active Publication Date: 2009-01-15
ARKRAY INC
View PDF12 Cites 26 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0009]An object of the present invention is, in analyzing a sample containing blood cells (e.g. in measuring the glucose level in blood), to suppress the influence of the blood cell concentration and to prevent the low value problem especially in the case of a sample having a high blood cell concentration.
[0010]Another object of the present invention is to reduce the variations of measurement results caused when the measurement time is shortened and to enhance the reproducibility of measurements particularly in a low concentration range.

Problems solved by technology

Such a delay is large in the whole blood with high blood cell concentration.
Therefore, in whole blood with high hematocrit, as the measurement time is shortened, the ratio of glucose which can be transferred from blood cells to blood serum (blood plasma) within the measurement time decreases, and the above-mentioned low-value problem becomes more significant.
When the reagent portion has poor solubility, the oxidoreductase and the electron mediator do not disperse uniformly in the liquid phase reaction system established when the reagent portion is dissolved by the blood, which causes variations in the measurement results.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Analytical instrument having improved arrangement of reagent section and analytical method
  • Analytical instrument having improved arrangement of reagent section and analytical method
  • Analytical instrument having improved arrangement of reagent section and analytical method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0098]In this example, in the measurement of a blood glucose level using a glucose sensor for colorimetry, the influence of the blood cell concentration (hematocrit(Hct)) in blood on the measurement result was examined. As the glucose sensor, sensors (1)-(3) having the structures described below were used. The influence of an Hct on the measurement result was examined based on the time course of absorbance and the bias after the lapse of a predetermined time period.

(Basic Structure of Glucose Sensor)

[0099]The basic structure of the glucose sensors (1)-(3) (excluding reagent portions) is shown in FIG. 14. Specifically, each of the glucose sensors (1)-(3) included a transparent substrate 2A, a transparent cover 4A bonded to the substrate via a spacer 3A, and a capillary 5A defined by the elements 2A-4A. As described in FIG. 14, the dimensions of the capillary 5A were 1.3 mm×9 mm×50 μm. The transparent substrate 2A and the transparent cover 4A were made of a PET material having a thick...

example 2

[0114]In this example, the time course of absorbance of each of the glucose sensors (1)-(3), which were prepared similarly to Example 1, was measured using five kinds of analytes having different glucose levels (Omg / dL, 113 mg / dL, 212 mg / dL, 430 mg / dL and 598 mg / dL). FIGS. 18A-18C show the results.

[0115]As will be understood from FIGS. 18A-18C, in the glucose sensors (1), (2) (inventive glucose sensors), the initial rise of absorbance is sharp and the time until the absorbance becomes close to a constant value is short for any of the analytes of different glucose levels, although the content of enzyme (activity reference) in the reagent portion of the glucose sensors (1), (2) was lower than that in the glucose sensor (3) (comparative glucose sensor). This also means that, as compared with the glucose sensor (3), the relationship between the absorbance and the glucose level in the glucose sensors (1), (2) has high linearity even when the measurement time is relatively short (e.g. wit...

example 3

[0116]The measurement reproducibility was evaluated based on the data obtained in Example 2. Specifically, the measurement reproducibility of each of the glucose sensors (1)-(3) was evaluated by computing the variations in the absorbance five seconds after the start of the measurement with respect to each of the analytes of different glucose levels. The computation results are given in Tables 8-10 below.

TABLE 8Glucose Sensor (1): Inventive Glucose SensorGlucose Level2125980 mg / dL113 mg / dLmg / dL430 mg / dLmg / dLAbsorbance0.0510.1400.2280.3950.5000.0470.1410.2220.3860.4920.0510.1410.2240.3880.5580.0530.1360.2370.3970.5130.0560.1430.2090.3850.475Average0.0520.1400.2240.3900.512S.D.0.000.000.010.000.03C.V.5.7%1.8%4.0%1.2%5.5%

TABLE 9Glucose Sensor (2): Inventive Glucose SensorGlucose Level2125980 mg / dL113 mg / dLmg / dL430 mg / dLmg / dLAbsorbance0.0410.1740.2540.4510.5750.0420.1740.2670.4570.5540.0360.1700.2710.4680.5570.0390.1740.2760.4380.5460.0460.1650.2700.4550.535Average0.0410.1720.2680.4540.5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Fractionaaaaaaaaaa
Fractionaaaaaaaaaa
Volumeaaaaaaaaaa
Login to View More

Abstract

The present invention relates to an analytical instrument (1) which includes a flow path (5) for moving a sample containing blood cells, an introduction port (50) for introducing the sample into the flow path (5), a reagent portion (51) arranged in the flow path (5), and an electron detection medium (52) for obtaining information necessary for analyzing an analysis target component contained in the sample in relation with an amount of electrons transferred. The reagent portion (51) contains an electron mediator for supplying an electron taken from the analysis target component in the sample to the electron detection medium (52), and at least part of the reagent portion is positioned adjacent to the introduction port (50).

Description

TECHNICAL FIELD[0001]The present invention relates to a technique for analyzing a particular component contained in a sample, in particular to a technique for measuring the glucose level in blood.BACKGROUND ART[0002]Examples of glucose sensors dealing with a sample of whole blood include sensors that utilize a colorimetric method or an electrode method (see Patent Documents 1 and 2, for example). In both of the above-described two techniques, the glucose level is determined by supplying electrons taken from glucose to an electron detection medium (color former or electrode) and grasping, from the outside of the glucose sensor, the amount of electrons supplied. Generally, the glucose sensor is designed to take electrons from glucose by oxidoreductase and supplying the electrons to the electron detection medium via an electron mediator.[0003]It is known that, in measuring the glucose level using whole blood, the measurement result is influenced by the blood cell concentration (hematoc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/54C12M1/34C12M1/36C12Q1/02C12Q1/00
CPCC12Q1/006
Inventor YAMAOKA, HIDEAKINAGAKAWA, KENJIHOSHIJIMA, MITSUHIROKIMURA, SADAAKI
Owner ARKRAY INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products