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Enzyme@ZIF-8@Fe3O4 magnetic nanoenzyme reactor and preparation method thereof

A magnetic nanometer, enzyme reactor technology, applied in chemical instruments and methods, biochemical equipment and methods, immobilized on/in organic carriers, etc., can solve the problem of increasing reaction costs, difficulty in recycling, and reaction speed. It can reduce the problems such as decrease, and achieve the effects of good reusability, simple preparation method, and improved thermal stability and acid-base stability.

Inactive Publication Date: 2017-10-20
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if the enzyme reacts under optimal conditions, the reaction speed will decrease with the prolongation of the reaction time, and after the free enzyme participates in the reaction, it is difficult to recycle and reuse, which increases the cost of the reaction

Method used

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  • Enzyme@ZIF-8@Fe3O4 magnetic nanoenzyme reactor and preparation method thereof
  • Enzyme@ZIF-8@Fe3O4 magnetic nanoenzyme reactor and preparation method thereof
  • Enzyme@ZIF-8@Fe3O4 magnetic nanoenzyme reactor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Citric acid modified Fe 3 o 4 magnetic nanoparticles

[0039] 1.0g (4mmol) magnetic Fe 3 o 4 Add the powder and 50mL ultrapure water into a 250mL three-neck flask, and ultrasonicate for 20 minutes under stirring to make the magnetic Fe 3 o 4 The powder is uniformly dispersed in ultrapure water, and then 4.5mL of 2.0mol / L citric acid aqueous solution is added, and the 2 Stir at 90°C for 90 minutes under protection, cool to room temperature, separate the product with a magnet, wash the product with pure water and dry it in a vacuum oven at 60°C for 5 hours to obtain citric acid-modified Fe 3 o 4 Magnetic nanoparticles (CA-Fe 3 o 4 ).

[0040] 2. Preparation of HRP@ZIF-8@Fe 3 o 4 Magnetic Nanozyme Reactor

[0041] 50.0mg citric acid modified Fe 3 o 4 After the magnetic nanoparticles are ground, place them in a 100mL beaker, add 10mL of 50% ethanol aqueous solution, and then add 55.0mg (0.29mmol) Zn(NO 3 ) 2 and 3mg mass fraction of 37% (0.029mmol) HCl aq...

Embodiment 2

[0043] In this example, the horseradish peroxidase in Example 1 was replaced with an equal mass of chloroperoxidase (CPO), and the other steps were the same as in Example 1 to obtain CPO@ZIF-8@Fe 3 o 4 Magnetic Nanozyme Reactor.

Embodiment 3

[0045] In this example, the horseradish peroxidase in Example 1 was replaced with an equal mass of cytochrome C (Cyt C), and the other steps were the same as in Example 1 to obtain Cyt C@ZIF-8@Fe 3 o 4 Magnetic Nanozyme Reactor.

[0046] The inventor adopts environmental scanning electron microscope-EDS energy spectrum, transmission electron microscope, X-ray diffractometer, Fourier transform infrared spectrometer, thermal analysis system to characterize the magnetic nanozyme reactor obtained in Examples 1~3 respectively, the results are shown in Figure 1-8 . Depend on figure 1 , 2 Visible, citrate-modified magnetic Fe 3 o 4 After coating ZIF-8, the size increased significantly, and the citric acid-modified magnetic Fe 3 o 4 The particle size of ZIF-8 is about 250-300nm, and the shell of ZIF-8 is about 50-80nm. The formed HRP@ZIF-8@Fe 3 o 4 The magnetic nanozyme reactor is spherical with uniform shape. Depend on image 3 Visible, HRP@ZIF-8@Fe 3 o 4 and CPO@ZIF-8@...

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Abstract

The invention discloses an enzyme@ZIF-8@Fe3O4 magnetic nanoenzyme reactor and a preparation method thereof. A denovo method is used for citric acid modification on the surface of the Fe3O4 magnetic nanometer particles; then, ZIF-8 is coated to form a ZIF-8@ZIF-8@Fe3O4 core-shell structure; meanwhile, chloroperoxidase, horse radish peroxidase or cytochrome C are immobilized onto ZIF-8 in the ZIF-8 coating process, so that the enzyme@ZIF-8@Fe3O4 magnetic nanoenzyme reactor with the magnetic separation effect is obtained, wherein the particle diameter of the citric acid modified Fe3O4 magnetic nanometer particles is 250 to 300 nm; the thickness of the ZIF-8 shells of the surface coated immobilization enzyme is 50 to 80 nm. The preparation method provided by the invention is simple and convenient; the thermal stability and the acid and base stability of the obtained enzyme@ZIF-8@Fe3O4 magnetic nanoenzyme reactor are obviously improved through being compared with that of free enzyme; good repeated use performance is realized.

Description

technical field [0001] The invention belongs to the technical field of enzyme immobilization, in particular to an easily separated magnetic nano-enzyme reactor enzyme@ZIF-8@Fe 3 o 4 and its preparation method. Background technique [0002] Enzyme-catalyzed reaction has been widely used in organic synthesis, pharmaceutical preparation and other fields due to its characteristics of mild conditions, high efficiency and orientation, and environmental friendliness. It is regarded as an important means of green synthesis of chemicals and is of great significance to the realization of sustainable development strategy . Enzymes are proteins composed of amino acids with a quaternary space structure. Its advanced structure is very sensitive to the environment. Many external factors such as physical factors (temperature, pressure), chemical factors (redox, organic solvents, pH, ionic strength, metal ions) and biological factors (enzyme modification, enzyme degradation) will have a r...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/02
CPCC12N11/14C07K14/80C12N9/0065C12N11/02C12Y111/01007C12Y111/0101
Inventor 蒋育澄宋艺超胡满成李淑妮翟全国
Owner SHAANXI NORMAL UNIV
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