28results about "Cytochromes" patented technology

The invention discloses a method for realizing high-efficiency selenium labeling of protein, which comprises the following steps of: in the process of expressing plasmids containing target protein through a prokaryote expression system, realizing high-efficiency replacement of cysteine sulfur atoms in the target protein by selenium atoms by regulating and controlling the ratio of selenium to sulfur elements in a culture medium; on the premise of keeping a good growth state of cells, the selenium labeling efficiency can reach 86%, and the protein expression quantity is stabilized at about 1mg / g (protein / thallus wet weight); structural analysis shows that the secondary structure of the obtained selenium-labeled protein is basically consistent with that of natural protein and is not obviously changed. The method is easy and convenient to operate, high in labeling efficiency, high in protein yield, good in repeatability and suitable for structural biology research such as nuclear magnetic resonance, an effective means is provided for research of the functional mechanism of sulfur atoms in biomacromolecules (including small peptides, polypeptides, peptide fragments, proteins and protein or peptide complexes) with selenium as a probe, and the method has a wide application prospect. Good scientific research application prospects and industrial popularization values are realized.

To provide recombinant adeno-associated viral vectors for treating Bietti Crystalline Dystrophy.SOLUTION: Provided are a recombinant adeno-associated vector comprising a codon-optimized sequence encoding CYP4 V2 linked to predetermine gene expression regulatory sequences, and its use in treating Bietti Crystalline Dystrophy (BCD).SELECTED DRAWING: None

The invention discloses application of a reducing agent in heme peroxidase catalytic reaction, the reducing agent is selected from ascorbic acid, dehydroascorbic acid, gallic acid and pyrogallic acid, and the reducing agent has a remarkable promotion effect on oxidation reaction and hydroxylation reaction catalyzed by heme peroxidase. A new scheme is provided for improving the catalytic oxidation efficiency of the heme peroxidase, and the development and application potential is huge.

The present disclosure provides a recombinant adeno-associated vector comprising a codon-optimized sequence encoding CYP4V2 linked to selected gene expression regulatory sequences and its use in treating Bietti Crystalline Dystrophy (BCD).

To provide recombinant adeno-associated viral vectors for treating Bietti Crystalline Dystrophy.SOLUTION: Provided are a recombinant adeno-associated vector comprising a codon-optimized sequence encoding CYP4 V2 linked to predetermine gene expression regulatory sequences, and its use in treating Bietti Crystalline Dystrophy (BCD).SELECTED DRAWING: None

The disclosure relates to transcription cassettes, expression vectors and microbial cells adapted for the expression of genes regulated by the RBA transcription factor.

The invention belongs to the field of protein separation and purification materials, and particularly discloses a polyoxometallate composite material and a preparation method and application thereof in protein separation and purification, 2 g of polyoxometallate C8H30N2O21W5Zr is added into 30 mL of deionized water, stirring is performed until the mixture is clear and transparent, 5 mL of a polyethyleneimine PEI solution is slowly dropwise added into the mixture under the stirring condition, stirring is performed under the room temperature condition for 7 h, and the polyoxometallate composite material is obtained. Washing with deionized water for several times, and drying at 60 DEG C for 24 hours to obtain a white solid. Compared with the prior art, the polyoxometallate composite material ZrW5-coated PEI modified by a high polymer material is prepared through electrostatic interaction among molecules and is applied to separation and purification of cytochrome c in pig hearts; results show that ZrW5-coated PEI has selective adsorption performance on cytochrome c under corresponding conditions. The novel method using ZrW5-coated PEI as the adsorbent has been successfully used for selective separation of cytochrome c in pig hearts, and shows great potential in separation and identification of cytochrome c in pig hearts.

To provide recombinant adeno-associated viral vectors for treating Bietti Crystalline Dystrophy.SOLUTION: Provided are a recombinant adeno-associated vector comprising a codon-optimized sequence encoding CYP4 V2 linked to predetermine gene expression regulatory sequences, and its use in treating Bietti Crystalline Dystrophy (BCD).SELECTED DRAWING: None

The application discloses a method for efficiently labeling proteins with selenium, which comprises the following steps: adjusting the ratio of selenium and sulfur in the culture medium during the expression of a plasmid containing a target protein by a prokaryotic expression system, and realizing the efficient replacement of selenium atoms for the cysteine sulfur atoms in the target protein; under the premise of maintaining the good growth state of cells, the selenium labeling efficiency can reach 86%, and the protein expression amount is stably about 1 mg / g (protein / biomass wet weight); structure analysis shows that the obtained selenium labeled protein is basically consistent with the natural protein in the secondary structure and does not change obviously. The method is simple in operation, high in labeling efficiency and protein yield, good in repeatability, and suitable for structural biology research such as nuclear magnetic resonance, provides an effective means for studying the function mechanism of sulfur atoms in biological macromolecules (including small peptides, polypeptides, peptide segments, proteins, and complexes of proteins or peptides) by using selenium as a probe, and has good scientific research application prospect and industrialization popularization value.

A polypeptide comprising a residue substituted by a non-canonical amino acid (ncAA) is provided. Methods for: (a) transferring an electron to an electrode, by coupling the polypeptide to an electrode; and (b) quantifying the amount of an analyte e.g., glucose are also provided.

The invention belongs to the field of microbial metabolic engineering, and discloses recombinant saccharomyces cerevisiae for synthesizing p-coumaric acid by using acetic acid as well as a preparation method and application of the recombinant saccharomyces cerevisiae. The recombinant saccharomyces cerevisiae has the following characteristics that: tyrosine lyase FjTAL, phenylalanine lyase AtPAL2, cinnamic acid 4-hydroxylase AtC4H, cytochrome P450 reductase AtATR2 and cytochrome B5 CYB5 are subjected to overexpression. The invention also provides a construction method and a domestication method of the recombinant saccharomyces cerevisiae. Compared with recombinant saccharomyces cerevisiae which is not evolved, the recombinant saccharomyces cerevisiae evolved adaptively can well grow in a culture medium which takes acetic acid as a unique carbon source, and p-coumaric acid with higher yield can be synthesized. The invention provides a feasible strategy for synthesizing p-coumaric acid, flavonoid or stilbene natural products by using acetic acid as a carbon source.

The application discloses a new use of cytochrome C in preparing an ELISA kit and relates to the field of biotechnology. The application uses cytochrome C (Cytochrome c, CC) and streptavidin (Streptavidin, SA) to construct a fusion protein, and the fusion protein has a good detection effect in an ELISA experiment. The fusion protein can simplify a production process, reduce production cost and has a good market conversion prospect.

The present invention relates to a vinyl compound containing an isatoic anhydride functional group, a copolymer thereof, a preparation method and applications thereof. The vinyl compound containing an isatoic anhydride functional group has a structure shown in formula (1). The water-soluble copolymer containing isatoic anhydride of the present invention is prepared by copolymerizing a vinyl compound containing an isatoic anhydride functional group with a water-soluble vinyl monomer. The water-soluble copolymer can be completely dissolved in water and does not undergo self-assembly, thereby effectively exposing the isatoic anhydride functional group. It has a high reactivity with the amino side groups in proteins and can conjugate with proteins efficiently, and can be used for modifying and modifying proteins. Moreover, the reaction conditions for modifying proteins are mild, the reaction is rapid, the modified protein complex has high activity, and has broad application prospects.

Disclosed in the present invention is an application of a reducing agent in a heme peroxidase catalytic reaction. The reducing agent is selected from ascorbic acid, dehydroascorbic acid, gallic acid and pyrogallic acid. The reducing agent has a significant promotion effect on oxidation reaction and hydroxylation reaction catalyzed by heme peroxidase.

The present invention relates to an optogenetic anti-inflammatory composition containing, as an active ingredient, nanoparticles including an agent for inhibiting the formation of the nod-like receptor protein 3 (NLRP3) inflammasome, wherein the nanoparticles contain: a vector including a polynucleotide encoding a truncated version of cryptochrome-interacting basic-helix-loop-helix 1 (CIBN)-NLRP3 fusion protein; and a vector including a polynucleotide encoding the cryptochrome 2 (CRY2) protein or a variant thereof. The optogenetic anti-inflammatory composition has excellent anti-inflammatory activity due to non-invasive light stimulation and can thus be highly effectively used in related fields.

The present disclosure relates to recombinant engineered polypeptides with cytochrome P450 (CYP) activity capable of selectively catalyzing the conversion of precursor compounds, carvacrol and / or thymol, to the product compound, thymohydroquinone (THQ), and recombinant host cells that heterologously express genes encoding these polypeptides (e.g., in yeast) and that can be used for enhanced fermentative bioproduction of the product compound THQ.

The present invention relates to a method for treating, preventing, or reducing a disease or disorder, e.g., hypertension, in a subject in need thereof. In embodiments, the method comprises co-administering to the subject an effective amount of a cytochrome P4503A4 (CYP3A4) inhibitor, an effective amount of a nitric oxide (NO) potentiator, and an effective amount of a phosphodiesterase-5 (PDE5) inhibitor. In some embodiments, the CYP3A4 inhibitor and the NO potentiator are the same compound.

The application belongs to the technical field of metal organic framework (MOF) immobilized enzyme, and particularly relates to a method for wrapping enzyme by seed orientation and application thereof. The application discloses a method for synthesizing enzyme@MOF composite by seed orientation, and based on the characteristics of MOF crystal growth, the seed orientation synthesis method is innovatively proposed. The addition of seed can greatly promote the synthesis efficiency of enzyme@MOF composite and greatly increase the encapsulation efficiency of enzyme. Meanwhile, the seed orientation synthesis method has no requirement on the chemical properties of enzyme / protein, and does not need to destructively modify the enzyme / protein to improve the encapsulation efficiency, so that the seed orientation synthesis method has good universality. The seed orientation synthesis method solves the problems of low universality and low efficiency in the existing enzyme@MOF composite synthesis method, and has an expected application prospect in the fields of biological catalysis, biological sensing, biological medicine and the like.

Particles comprising a protein and optionally an activator, for example, particles comprising whey protein hydrolysate and optionally tryptophan, are described herein. Methods for preparing and using particles comprising a protein and optionally an activator are also described herein.

The application provides a CYBB lentivirus vector, a stem cell transfected by the lentivirus vector and a preparation method and application thereof. The lentivirus vector comprises an hEF1 alpha promoter and CYBB tandem co-expression. The lentivirus vector of the application carries a CYBB gene, realizes safe and efficient expression of the carried CYBB gene in differentiated or undifferentiated stem cells under the start of an hEF1 alpha promoter, and meanwhile, the stem cell serves as a potential delivery carrier, and the expression amount of the CYBB gene in a transgenic cell is improved.

A nanowire may be isolated from a bacterium, wherein the nanowire includes c-type heme subunits or pili proteins and is capable of establishing an electrical connection with an insoluble electron acceptor. The c-type heme subunits may comprise OmcS or OmcZ cytochrome subunits or any combination thereof. The pili proteins may have an amino acid sequence with at least one residue modified, from a wild-type strain of the microorganism, to include an aromatic amino acid. The nanowire may have metallic or semiconducting conductive properties, and may be integrated into an electronic device.

Provided are a microorganism for producing L-amino acid, having increased cytochrome C activity, and an L-amino acid production method using the microorganism.

The invention relates to an electromicrobial system for synthesis of hydrogen, comprising: a gram-negative bacterial cell modified for synthesis of hydrogen, wherein the bacterial cell comprises: i) inner membrane-bound rhodopsin for proton-pumping, ii) membrane-bound hydrogenase, and iii) optionally an electron mediator located in the periplasm and / or on the outer membrane of the bacterial cell; and an electron source for donation of electrons to the bacterial cell.