New method for preparing cytochrome C

A cytochrome and extraction technology, applied in the field of cytochrome C preparation, can solve the problems of low yield, severe extraction conditions, long time-consuming and the like, and achieve the effects of simple steps, mild extraction conditions and low cost

Inactive Publication Date: 2010-05-19
YANTAI DONGCHENG PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing cytochrome C extraction method is to extract with sulfuric acid aqueous solutio

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1, the preparation method of cytochrome C, minced 5 kg of pig heart, added 15 L of 2% aluminum sulfate solution, controlled the pH value at 3, lowered the temperature to 0 ° C, extracted for 2 hours, and then filtered to obtain the extracted The solution was adsorbed by zeolite, eluted, salted out, desalted by ultrafiltration, and freeze-dried to obtain 0.9 g of the product.

Embodiment 2

[0012] Example 2, mince 30kg of beef heart, put it in a sandwich reaction tank, add 60L of 3% aluminum sulfate solution, control the pH value at 6, control the temperature to 15°C, extract for 3 hours, and then filter to remove the heart slag Then it can be extracted twice with aluminum sulfate solution according to the aforementioned method, and all the obtained extracts are adsorbed by zeolite, eluted, salted out, desalted by ultrafiltration, and freeze-dried to obtain 5.9 g of the product.

Embodiment 3

[0013] Example 3, crush 100kg of horse heart, add 400L of 6% aluminum sulfate solution, control the pH value at 5, lower the temperature to 30°C, extract for 4 hours, and then filter, the heart slag can be pressed by aluminum sulfate solution The aforementioned methods were extracted 8 times, and all the obtained extracts were adsorbed by zeolite, eluted, salted out, desalted by ultrafiltration, and freeze-dried to obtain 18.5 g of the product.

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PUM

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Abstract

The invention discloses a method for extracting cytochrome C, which comprises the following steps of: using the cardiac muscle of a mammal as a raw material; extracting the cytochrome C by adopting an extraction method using an aluminum sulfate solution, wherein the concentration of aluminum sulfate in the extraction process is controlled to be between 0.1 and 10 percent, the volume of the aluminum sulfate solution is controlled to be 1 to 5 times that of the cardiac muscle, the extraction pH value is controlled to be between 3 and 6, the reaction temperature is controlled to be between 0 and 30 DEG C, and the extraction time is controlled to be 1 to 4 hours; and performing adsorption elution to finally obtain the cytochrome C. The method has the characteristics of low cost, simple steps, mild extraction condition, fewer impurities, pollution-free reactants, and suitability for industrial mass production.

Description

technical field [0001] The invention belongs to the technical field of chemical industry, and in particular relates to a preparation method of cytochrome C. Background technique [0002] Cytochrome C is an iron porphyrin-containing protein that acts as an electron transporter in biological oxidation processes. Its principle of action is that in the presence of enzymes, it has a rapid enzymatic effect on the oxidation and reduction of tissues. Normally, exogenous cytochrome C cannot enter healthy cells, but under hypoxia, the permeability of the cell membrane increases, and cytochrome C may enter cells and mitochondria, thereby enhancing cell oxidation and improving oxygen utilization. [0003] Cytochrome C injection currently available on the market is used for first aid and adjuvant medication for tissue hypoxia, such as carbon monoxide poisoning, hypnotics poisoning, neonatal asphyxia, severe shock hypoxia, dyspnea caused by anesthesia and lung diseases, high mountain hyp...

Claims

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Application Information

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IPC IPC(8): C07K14/80C07K1/14
Inventor 由守谊齐东绮王建强张立勇
Owner YANTAI DONGCHENG PHARMA GRP
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