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Preparation method of artemisinin molecular imprinting photoelectrochemical sensor

A technology of molecular imprinting and photoelectrochemistry, which is applied in the field of analysis, detection and sensors, can solve the problems of poor selectivity, complicated operation and high cost, and achieve the effect of low cost, simple operation and high selectivity

Active Publication Date: 2016-11-16
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These traditional detection methods generally have defects such as complex operation, poor selectivity, high cost, and time-consuming. It has become a very meaningful research work to develop simple and efficient detection methods for artemisinin.

Method used

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  • Preparation method of artemisinin molecular imprinting photoelectrochemical sensor

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Experimental program
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Effect test

Embodiment 1

[0028] Polish the glassy carbon electrode with 0.3 μm and 0.05 μm alumina paste, then put it into a mixed solution of distilled water and acetaldehyde for ultrasonic cleaning, take it out and blow it dry with nitrogen. Immerse the treated glassy carbon electrode in a deoxyphosphate buffer solution containing 14 μM artemisinin, 0.075 mM acrylamide, 0.75 mM ethylene glycol dimethacrylate, 0.06 mM azobisisobutyronitrile, and 0.1 M potassium chloride . In the range of -0.2 ~ 1.2V, the scan rate is 100mVs -1 , using cyclic voltammetry to scan for 7 to 10 cycles until the scanning curve is stable. Soak the electrode in a mixed solution of acetic acid and methanol for 1 hour to elute the template molecules to obtain an artemisinin molecularly imprinted electrode. At 25°C, immerse the prepared molecularly imprinted electrode in a solution containing 4 mM artemisinin to cover the cavity for 15 min, then put it in a solution containing cytochrome C-labeled artemisinin for 20 min, and ...

Embodiment 2

[0030] Polish the glassy carbon electrode with 0.3 μm and 0.05 μm alumina paste, then put it into a mixed solution of distilled water and acetaldehyde for ultrasonic cleaning, take it out and blow it dry with nitrogen. Immerse the treated glassy carbon electrode in a deoxyphosphate buffer solution containing 13.6 μM artemisinin, 0.065 mM acrylamide, 0.78 mM ethylene glycol dimethacrylate, 0.056 mM azobisisobutyronitrile, and 0.1 M potassium chloride middle. In the range of -0.2 ~ 1.2V, the scan rate is 100mVs -1 , using cyclic voltammetry to scan for 10 to 15 cycles until the scanning curve is stable. Soak the electrode in a mixed solution of acetic acid and methanol for 1 hour to elute the template molecules to obtain an artemisinin molecularly imprinted electrode. At 25°C, immerse the prepared molecularly imprinted electrode in a solution containing 4 mM artemisinin to cover the cavity for 20 min, then put it in a solution containing cytochrome C-labeled artemisinin for 30...

Embodiment 3

[0032]Polish the glassy carbon electrode with 0.3 μm and 0.05 μm alumina paste, then put it into a mixed solution of distilled water and acetaldehyde for ultrasonic cleaning, take it out and blow it dry with nitrogen. Immerse the treated glassy carbon electrode in a deoxyphosphate buffer solution containing 14 μM artemisinin, 0.07 mM acrylamide, 0.76 mM ethylene glycol dimethacrylate, 0.055 mM azobisisobutyronitrile, and 0.1 M potassium chloride . In the range of -0.2 ~ 1.2V, the scan rate is 100mVs -1 , using cyclic voltammetry to scan for 15 to 20 cycles until the scanning curve is stable. Soak the electrode in a mixed solution of acetic acid and methanol for 1 hour to elute the template molecules to obtain an artemisinin molecularly imprinted electrode. At 25°C, the prepared molecularly imprinted electrode was immersed in a solution containing 5.5 mM artemisinin to cover the cavity for 15 min, then it was placed in a solution containing cytochrome C-labeled artemisinin fo...

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Abstract

Disclosed is a preparation method of an artemisinin molecular imprinted photoelectrochemical sensor. The invention discloses the preparation method of an artemisinin molecular imprinted photoelectrochemical sensor based on the action of cytochrome C and pyronine B. The preparation method comprises the following steps: 1) performing electrochemical polymerization on a glassy carbon electrode to prepare a molecular imprinted polymer film and performing elution to remove template molecules and obtain imprinted holes; 2) immersing imprinted polymer in an artemisinin-contained solution to shelter holes in the imprinted film, putting the obtained product in a solution containing cytochrome-C-marked template molecules for replacing the template molecules in the imprinted holes and causing a competitive reaction, and putting the obtained product in a potassium-ferricyanide-contained electrolyte solution to observe the change of electrical signals; and 3) achieving photoelectrochemical detection of artemisinin with decrease of the cytochrome-C-marked template molecules in the solution and reduction of the fluorescence of pyronine B taken as a substrate. The obtained artemisinin molecular imprinted sensor has advantages of simple operation, high selectivity, and low cost. Combination of optical and electrical signals is adopted, and the sensor can be used as a novel molecular imprinted sensor used for efficient detection of artemisinin in a biological sample and a complex system.

Description

technical field [0001] The invention belongs to the technical field of analysis, detection and sensors, and in particular relates to a preparation method of an artemisinin molecularly imprinted photoelectrochemical sensor based on the interaction between cytochrome C and pyronine B. Background technique [0002] Artemisinin is a naturally occurring sesquiterpene endoperoxide with pharmacological activities such as antibacterial and antimalarial. Artemisinin has received much attention in the treatment of malaria due to the increased resistance of Plasmodium falciparum strains to traditional quinoline-based antimalarial drugs. Artemisinin and its derivatives are regarded as the most potential medicinal ingredients in the treatment of antimalarials. The application of artemisinin in clinical medicine has attracted increasing attention, and has also attracted researchers to actively develop methods for the detection of artemisinin with high selectivity and high sensitivity. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/643
Inventor 王宗花郭慧君桂日军金辉徐显朕
Owner QINGDAO UNIV
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