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Sensing material based on up-conversion nanoparticle and preparation method of sensing material

A nanoparticle and sensing material technology, which is applied in the field of preparation of upconversion nanoparticle sensing materials, can solve the problems of lack of specificity and inability to image target cells, and achieve uniform particle size, high selectivity, and broad application prospects Effect

Active Publication Date: 2015-05-20
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this UCNPs lack specificity and cannot target cells for imaging

Method used

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  • Sensing material based on up-conversion nanoparticle and preparation method of sensing material
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  • Sensing material based on up-conversion nanoparticle and preparation method of sensing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The invention provides a method for preparing a sensing material based on upconversion nanoparticles, and the specific preparation route is as follows: figure 1 Shown:

[0033] (1) Weigh 0.78mmol of yttrium acetate, 0.2mmol of ytterbium acetate and 0.02mmol of erbium acetate and dissolve them in 6mL of oleic acid and 17mL of octadecene. The mixture is heated to 160°C to form a transparent solution, which is naturally cooled to room temperature. Add 2.5mmol NaOH and 4mmol NH dropwise 4 A solution of F in 10 mL of methanol was stirred at room temperature for 30 min. Then, methanol was gradually removed by heating. After vacuuming at 100°C for 10 minutes, it was heated to 300°C under the protection of argon and maintained for 1h. After the resulting solution was naturally cooled, the oil-soluble up-converting nanoparticles were precipitated in ethanol;

[0034] (2) Disperse 100 mg of the above-prepared up-converting nanoparticles in 3 mL of toluene for later use. Weigh...

Embodiment 2

[0045] To study the adsorption performance of the sensing material (such as Figure 7 shown). Add 2.5 mg of UCNPsMIP or UCNPsNIP into a 4 mL centrifuge tube, and then add 2 mL of cytochrome C (PBS, pH 7.4) at concentrations of 4, 8, 12, 16, 20, and 24 μM. The mixture was shaken at room temperature for a period of time, and then its fluorescence value was measured with a fluorescence spectrophotometer connected with a 980nm exciter. From Figure 7 It can be seen that, with the increase of cytochrome C concentration, the fluorescence intensity weakens, and at the same concentration of cytochrome C, the fluorescence quenching of UCNPsMIP is greater than that of UCNPsNIP.

Embodiment 3

[0047] The dynamic performance of the sensing material was studied. Weigh 2.5 mg of the UCNPsMIP prepared in Example 1 and place it in a 4 mL centrifuge tube, then add 2 mL of 8 μM cytochrome C (PBS, pH 7.4), shake at room temperature for 0.5, 1, 2, 4, 6, 8, After 10, 12, and 14 hours, measure the fluorescence value with a fluorescence spectrophotometer connected with an external 980nm exciter. Such as Figure 8 As shown, the adsorption of UCNPsMIP to the target reached equilibrium in 10 h.

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Abstract

The invention provides a sensing material based on an up-conversion nanoparticle. The sensing material comprises cytochrome C and polyacrylic acid modified up-conversion nano-particles (UCNPs). The invention also discloses a preparation method of the sensing material. The sensing material has the advantages that the polyacrylic acid modified UCNPs are introduced to be used as a carrier for providing a relatively-large surface area and are also introduced to be used as a fluorescent material for providing a fluorescent signal. The molecularly-imprinted sensing material synthesized by using a sol-gel method is small and uniform in particle size and has relatively high selectivity for cytochrome C. The sensing material combines strong fluorescence of the UCNPs and the specificity recognition of a molecularly-imprinted polymer so as to be wide in application prospect.

Description

technical field [0001] The invention belongs to the technical field of preparation of optical sensing materials, in particular to a preparation method based on up-conversion nano particle sensing materials. Background technique [0002] In recent years, with the continuous development of nanotechnology, fluorescent nanomaterials have attracted the attention of researchers. Up-converting fluorescent nanoparticles (UCNPs) are developing rapidly as a new type of luminescent material. Compared with traditional fluorescent materials (such as quantum dots), in addition to the advantages of lower toxicity, narrower excitation region, better stability of emitted fluorescence, and lower photobleaching, UCNPs are characterized by the photon energy absorbed by the nanomaterial. Convert low-energy infrared or near-infrared light into high-energy ultraviolet or visible light below the photon energy it emits. Therefore, UCNPs are most widely used in biological fields such as bioimaging ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/85G01N21/64C09K11/02B82Y30/00B82Y40/00
Inventor 王硕郭婷邓启良刘冰王俊平
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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