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Hypocrellin composite nanoparticle and preparation method thereof

A technology of composite nanoparticles and oleocanthin, applied in the fields of materials and biomedicine, can solve problems such as limiting clinical application

Pending Publication Date: 2022-03-22
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it takes time for these inorganic materials to degrade in the body, and the dosage exceeds a certain range, which will cause non-negligible acute and long-term toxicity, which limits their clinical application.

Method used

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  • Hypocrellin composite nanoparticle and preparation method thereof
  • Hypocrellin composite nanoparticle and preparation method thereof
  • Hypocrellin composite nanoparticle and preparation method thereof

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preparation example Construction

[0032] The preparation method of Hypocretin composite nanoparticles of the present invention comprises the following steps:

[0033] (1), the preparation of hypocrellin nanoparticle suspension: the hypocrellin is dissolved in dimethyl sulfoxide (DMSO) solution, obtains the hypocrellin solution (the DMSO that is dissolved with hypocremin) solution), and then drop the hypocretin solution into phosphate buffered saline (PBS), the dropping process, supplemented by ultrasonic dispersion treatment for 5-30min, and then dialyzed for 2-12h to remove DMSO, the dialyzed external fluid is PBS , to obtain a suspension of hypocrellin nanoparticles;

[0034] Alternatively, dispersing hypocretin in an aqueous solution of poloxamer (trade name: Pluronic) and ultrasonically dispersing to obtain a suspension of hypocretin nanoparticles;

[0035] (2), the preparation of hypocretin composite nanoparticles: the hypocretin nanoparticle suspension of step (1) is mixed with the suspension of nano th...

Embodiment 1

[0041] The preparation method of the hypocrellin A composite nanoparticle of the present embodiment comprises the steps:

[0042] (1), preparation of hypocrellin A nanoparticle suspension: take 2mg hypocrellin A (Hypocrellin A, be called for short HA), and dissolve in the dimethyl sulfoxide (DMSO) solution of 1mL, obtain 2mg / mL Hypocretin A solution, then add 5μL of Hypocretin A solution dropwise into 1mL of phosphate buffered saline (PBS), and disperse with ultrasonic waves while adding drops. After treatment for 10 minutes, the sample was dialyzed for 2 hours to obtain a suspension of 10 μg / mL hypocrellin A nanoparticles that could be dispersed in PBS.

[0043] (2), extract the thylakoid in the spinach leaf cell, prepare the suspension of nano thylakoid membrane (Thylakoid membrane, be called for short TM); The preparation process is: preparation buffer solution (Tricine of 20mmol / L, 0.4mol / L Sucrose, 2mmol / L ascorbic acid, 2mmol / L MgCl 2 , 40mmol / L NaCl and 0.2% BSA), 100...

Embodiment 2

[0047] The preparation method of the hypocrellin A composite nanoparticle of the present embodiment comprises the steps:

[0048] (1), preparation of hypocrellin A nanoparticle suspension: prepare 3 parts of ethanol solution containing 0.5mg / mL hypocrellin A, each 0.5mL, place in a small dry beaker, and evaporate to dryness Ethanol to obtain hypocrellin A. Prepare the pluronic F68 aqueous solution containing 5mg / mL at the same time, add the pluronic F68 aqueous solution in the above-mentioned 3 small beakers containing hypocrellin A precipitation, the mass ratio of hypocrellin A and pluronic F68 is 50:1, 1 respectively. :1, 1:100. Such as figure 2 As shown, even if the amount of pluronic F68 is small, pluronic F68 can better improve the dispersibility of hypocrellin A in the water phase.

[0049] (2), extract the thylakoid in the spinach leaf cell, prepare the suspension of nano thylakoid membrane (Thylakoid membrane, be called for short TM); The preparation process is: pr...

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Abstract

The invention provides hypocrellin composite nano particles and a preparation method thereof.The nano particles are composed of hypocrellin nano particles and nano capsule-like membranes coating the surfaces of the hypocrellin nano particles, and the preparation method comprises the steps that water-insoluble hypocrellin is dissolved in a dimethyl sulfoxide solution, then the solution is dropwise added into a phosphate buffer solution, and the hypocrellin nano particles are obtained. Performing ultrasonic dispersion treatment and dialysis, or dispersing the hypocrellin in a poloxamer aqueous solution, and performing ultrasonic dispersion to obtain a hypocrellin nanoparticle suspension; then modifying the surface of the hypocrellin nanoparticle suspension with a suspension of a thylakoid membrane extracted from green plant cells, or modifying the surface of the hypocrellin nanoparticle suspension with a suspension of a thylakoid membrane extracted from microalgae cells, and extruding the hypocrellin nanoparticle suspension through a microfiltration membrane; the composite nanoparticles have the capability of producing oxygen through photosynthesis under illumination, and can provide oxygen for the photodynamic effect or the sonodynamic effect of the hypocrellin, so that the level of singlet oxygen produced by the hypocrellin under the action of illumination or ultrasonic waves is improved.

Description

technical field [0001] The invention belongs to the technical field of materials and biomedicine, and in particular relates to a composite nanoparticle of hypocrellin and a preparation method thereof, in particular to a method of coating a nano thylakoid film on the surface of hypocrellin to obtain A method for preparing hypocrellin composite nanoparticles with stronger photodynamic effect or stronger sonodynamic effect. Background technique [0002] Hypocretin, including hypocrellin A and hypocrellin B, is a 3,10-dihydroxy-4,9-perylenequinone derivative extracted from natural bamboo red fungus, which is a natural photosensitive It can efficiently produce singlet oxygen under light, has the characteristics of strong phototoxicity, low dark toxicity, and fast metabolism in the body, and can play an important role in photodynamic therapy of tumors and skin diseases. Hypocretin not only can generate reactive oxygen species under light, but also can generate reactive oxygen spe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/46A61P35/00B82Y5/00B82Y40/00B82Y20/00
CPCA61K9/5068A61K41/0057A61K41/0033A61P35/00B82Y5/00B82Y40/00B82Y20/00
Inventor 储茂泉桂馨
Owner TONGJI UNIV
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