An artificial thylakoid microfluidic chip and its preparation method and application

A microfluidic chip and thylakoid technology, applied in the field of biochemistry, can solve the problems of poor compatibility between photocatalytic materials and biocatalytic enzymes, low utilization rate of oxidoreductase, complicated recovery steps, etc. The effect of reaction efficiency, high utilization rate and easy recovery

Active Publication Date: 2022-05-17
QILU UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides an artificial thylakoid microfluidic chip and its preparation method, which is applied in the regeneration of nicotinyl coenzyme with high efficiency photocatalysis, and solves the problem of photocatalysis in the existing photocatalytic coenzyme regeneration system. The material recovery steps are complicated, the utilization rate of oxidoreductase is low, the stability is poor, it is not easy to recycle, and the compatibility between photocatalytic materials and biocatalytic enzymes is poor.

Method used

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  • An artificial thylakoid microfluidic chip and its preparation method and application
  • An artificial thylakoid microfluidic chip and its preparation method and application
  • An artificial thylakoid microfluidic chip and its preparation method and application

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Embodiment 1

[0038] The artificial thylakoid microfluidic chip is composed of two microfluidic chip channels bonded up and down to form a cavity 3 composed of an upper structure 1 and a lower structure 2, and the inner surface of the cavity 3 upper structure 1 Coated with mpg-C 3 N 4 The inner surface of the lower structure 2 of the cavity 3 is firstly added with 3-aminopropyltriethoxysilane, then dopamine is added for chemical modification, and then the inner surface of the lower structure 2 of the cavity 3 is injected with a concentration of 1mg / ml of glutamate dehydrogenase solution.

[0039] Two ends of the cavity 3 are provided with a sample inlet 4 and a sample outlet 5 .

[0040] The preparation method of the artificial thylakoid microfluidic chip comprises the following steps:

[0041] (1) Prepare the PDMS prepolymer and the curing agent according to the mass ratio of 10:1, stir evenly, remove the air bubbles with a vacuum pump, and prepare the mixed PDMS mixture;

[0042] (2)...

Embodiment 2

[0048] The artificial thylakoid microfluidic chip is composed of two microfluidic chip channels bonded up and down to form a cavity 3 composed of an upper structure 1 and a lower structure 2, and the inner surface of the cavity 3 upper structure 1 Coated with 2D COF, the inner surface of the lower structure 2 of the cavity 3 is firstly added with 3-aminopropyltriethoxysilane, and then dopamine is added for chemical modification, and then the inner surface of the lower structure 2 of the cavity 3 A solution of glutamate dehydrogenase at a concentration of 8 mg / ml was injected into the surface.

[0049] Two ends of the cavity 3 are provided with a sample inlet 4 and a sample outlet 5 .

[0050] The preparation method of the artificial thylakoid microfluidic chip comprises the following steps:

[0051] (1) Prepare the PDMS prepolymer and the curing agent according to the mass ratio of 10:1, stir evenly, remove the air bubbles with a vacuum pump, and prepare the mixed PDMS mixtur...

Embodiment 3

[0058] The artificial thylakoid microfluidic chip is composed of two microfluidic chip channels bonded up and down to form a cavity 3 composed of an upper structure 1 and a lower structure 2, and the inner surface of the cavity 3 upper structure 1 coated with g-C 3 N 4 Quantum dots, first add 3-aminopropyltriethoxysilane to the inner surface of the lower structure 2 of the cavity 3, then add dopamine for chemical modification, and then inject a concentration of 15mg / ml glutamate dehydrogenase solution.

[0059] Two ends of the cavity 3 are provided with a sample inlet 4 and a sample outlet 5 .

[0060] The preparation method of the artificial thylakoid microfluidic chip comprises the following steps:

[0061] (1) Prepare the PDMS prepolymer and the curing agent according to the mass ratio of 10:1, stir evenly, remove the air bubbles with a vacuum pump, and prepare the mixed PDMS mixture;

[0062] (2) The SU-8 mold is made by soft etching technology, and two identical micro...

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Abstract

The invention discloses an artificial thylakoid microfluidic chip and a preparation method thereof. There are photocatalytic materials, the inner surface of the lower structure of the artificial thylakoid microfluidic chip channel is fixed with oxidoreductase, and the fixed materials on the surface of the upper and lower structures do not contact and interfere with each other, and are applied in high-efficiency photocatalytic nicotinyl coenzyme regeneration aspect. The existing photocatalytic coenzyme regeneration system solves the shortcomings of complex photocatalytic material recovery steps, low utilization rate of oxidoreductase, poor stability, difficult recovery, and poor compatibility between photocatalytic materials and biocatalytic enzymes.

Description

[0001] field of invention [0002] The invention belongs to the technical field of biochemistry, and in particular relates to an artificial thylakoid microfluidic chip and a preparation method and application thereof. Background technique [0003] At present, in the application of artificial thylakoids, regarding the regeneration of nicotinyl coenzyme and the reaction of oxidoreductase, some of them are connected in series before and after the two-part reaction, and some of them add oxidoreductase in the form of free enzyme to the suspension system of nicotinyl coenzyme regeneration. The two reaction methods have disadvantages such as complex recovery steps of photocatalytic materials, low utilization rate of oxidoreductase, poor stability, difficult recovery, and poor compatibility between photocatalytic materials and biocatalytic enzymes. Contents of the invention [0004] Aiming at the deficiencies of the prior art, the present invention provides an artificial thylakoid m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00C12M1/40C12M1/00C12P13/14C12P19/36
CPCB01L3/5027B01L3/502707B01L3/502715C12M21/18C12M23/16C12P13/14C12P19/36B01L2300/0809B01L2300/0883B01L2300/0887B01L2300/12B01L2300/168B01L2400/046
Inventor 黄晓文刘洋林惠超
Owner QILU UNIV OF TECH
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