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A kind of thylakoid lipid solid support membrane and its construction method

A construction method and supporting membrane technology, applied in chemical instruments and methods, membrane technology, semi-permeable membrane separation, etc., can solve the problems of not being able to simulate the microenvironment well, and achieve the effect of improving in vitro activity

Active Publication Date: 2021-05-25
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the environment constructed by these methods is far from the in vivo transmembrane microenvironment of PSII, and cannot simulate its microenvironment well.

Method used

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  • A kind of thylakoid lipid solid support membrane and its construction method
  • A kind of thylakoid lipid solid support membrane and its construction method
  • A kind of thylakoid lipid solid support membrane and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Modification of the substrate surface by chitosan

[0039] A gold-plated silicon wafer was selected as the substrate, and the piranha solution (piranha solution, H 2 SO 4 :H 2 o 2 =3:1 (v / v)) this substrate is cleaned, after that, the chitosan aqueous solution that concentration is 8mg / ml is added dropwise on the substrate surface that cleans, room temperature hatches 40min, forms the gold of chitosan modification. Surface base.

[0040] 2. Preparation of PSII-integrated thylakoid lipid vesicles

[0041] (1) Dissolve monogalactosyl diglyceride (MGDG), digalactosyl diglyceride (DGDG), thioisorhamnose diglyceride (SQDG) and phosphatidylglyceride (PG) in chloroform according to Close to its ratio in thylakoids and mix in brown vials (MGDG 50%, DGDG 31%, SQDG 8.3%, PG 10.7%), for the experimental group that needs to add cholesterol, at this time add as total thylakoid lipids 5% cholesterol by mole;

[0042] (2) Nitrogen gas is slowly introduced into the bottle, an...

Embodiment 2

[0054] 1. Modification of the substrate surface by chitosan

[0055] A gold-plated silicon wafer was selected as the substrate, and the piranha solution (piranha solution, H 2 SO 4 :H 2 o 2 =3:1 (v / v)) to clean the substrate, after that, the chitosan aqueous solution with a concentration of 10mg / ml is added dropwise on the cleaned substrate surface, and incubated at room temperature for 20min to form chitosan-modified gold Surface base.

[0056] 2. Preparation of PSII-integrated thylakoid lipid vesicles

[0057] (1) Dissolve monogalactosyl diglyceride (MGDG), digalactosyl diglyceride (DGDG), thioisorhamnose diglyceride (SQDG) and phosphatidylglyceride (PG) in chloroform according to Close to its ratio in thylakoids and mix in brown vials (MGDG 48%, DGDG 33%, SQDG 7.3%, PG 11.7%), for the experimental group that needs to add cholesterol, add as total thylakoid lipids at the same time 3% cholesterol by mole;

[0058] (2) Nitrogen gas is slowly introduced into the bottle, ...

Embodiment 3

[0069] 1. Modification of the substrate surface by chitosan

[0070] A gold-plated silicon wafer was selected as the substrate, and the piranha solution (piranha solution, H 2 SO 4 :H 2 o 2 =3:1 (v / v)) to clean the substrate, after that, the chitosan aqueous solution with a concentration of 15mg / ml is added dropwise on the cleaned substrate surface, and incubated at room temperature for 10min to form chitosan-modified gold Surface base.

[0071] 2. Preparation of PSII-integrated thylakoid lipid vesicles

[0072] (1) Dissolve monogalactosyl diglyceride (MGDG), digalactosyl diglyceride (DGDG), thioisorhamnose diglyceride (SQDG) and phosphatidylglyceride (PG) in chloroform according to Close to its ratio in thylakoids, mix them in brown vials (MGDG 52%, DGDG 29%, SQDG 9.3%, PG 9.7%). For the experimental group that needs to add cholesterol, add as total thylakoid lipids at the same time. 8% cholesterol by mole;

[0073] (2) Nitrogen gas is slowly introduced into the bottle...

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Abstract

The invention discloses a thylakoid lipid solid support membrane and a construction method thereof. The construction method includes: providing a substrate, and modifying the surface of the substrate with hydrophilic polymer molecules to form a substrate modified by hydrophilic polymer molecules; providing thylakoid lipid vesicles, and integrating PSII into the thylakoid In lipid vesicles, thylakoid lipid vesicles integrated with PSII are formed; the content of cholesterol in thylakoid lipid vesicles is 3-8% mol, Ca in solution 2+ When the concentration is 15-25 mM, the thylakoid lipid vesicle integrated with PSII is incubated on the substrate surface modified by the hydrophilic polymer molecules to obtain a solid support membrane of thylakoid lipid integrated with PSII. With the method for constructing thylakoid lipid solid support membrane provided by the present invention, the in vitro activity of PSII can be significantly improved.

Description

technical field [0001] The invention relates to a method for constructing a thylakoid lipid solid support membrane, in particular to a thylakoid lipid solid support membrane and a construction method thereof, and belongs to the technical field of bionic membrane preparation. Background technique [0002] PSII (Photosystem II) is a photosynthesis-related enzyme, mainly present on the thylakoid membrane in plant leaf cells, and is a transmembrane protein. PSII has the characteristics of splitting water molecules under light conditions, generating oxygen and protons, and transferring the released electrons to plastoquinone. Based on this unique property of PSII, it has important potential significance in the research of clean energy field. For example, the combination of PSII with inorganic catalysts is used to generate hydrogen; PSII-based photoelectrochemical cells are used to generate photocurrent. In these existing reports, researchers generally improve the stability of P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D67/00B01D69/10C02F1/44
CPCB01D67/0002B01D69/10C02F1/44
Inventor 黄雷郝王平陈艳艳戴建武
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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