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Application of a kind of nano thylakoid

A technology of thylakoid and nanotechnology, which is applied in the application field of nanothylakoids, can solve the problems of complex preparation of nanomaterials, poor biological safety, and large side effects, and achieve the effect of overcoming tumor hypoxia, low cost, and small side effects

Inactive Publication Date: 2020-09-01
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome tumor hypoxia, improve the efficiency of tumor treatment, and solve the problems of complex preparation of existing anti-tumor nanomaterials, poor biological safety, and large side effects, the present invention provides an application of nano thylakoids in the preparation of anti-tumor drugs ; Aim to overcome tumor hypoxia, improve anti-tumor effect and biological safety and reduce side effects

Method used

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  • Application of a kind of nano thylakoid
  • Application of a kind of nano thylakoid
  • Application of a kind of nano thylakoid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Step (1) preparation of nano thylakoids:

[0053] Nanothylakoids were prepared by osmotic lysis. The specific method is as follows: take 100g of fresh spinach leaves and put them into a mortar to grind into uniform fragments, then add 300mL of pH to the mortar and be a homogeneous buffer solution of 7.8,

[0054] After mixing, filter the filtrate with ten layers of gauze. Then the filtrate was collected, and the filtrate was centrifuged at 8000 rpm for 10 minutes to collect the precipitate to obtain the original chloroplast. The obtained original chloroplasts were redispersed in a lysis buffer with a concentration of 10mM HEPES (pH 8.0) and kept in a refrigerator at 4°C for 2h, and then the lysed solution was centrifuged at 8000rpm for 10min to remove the upper layer of cytoplasm matrix solution, and collect the precipitate. Then the precipitate was washed twice with the above HEPES buffer solution, redispersed in secondary water with a pH of 7.4, and ultrasonically ...

Embodiment 2

[0068] Preparation of nanothylakoids:

[0069] Nanothylakoids were prepared by osmotic lysis. The specific method is as follows: Weigh 100g of fresh spinach leaves and grind them into uniform pieces in a mortar, then add 300mL of a homogeneous buffer solution with a pH of 8.0 to the mortar, mix well, and filter with ten layers of gauze to collect the filtrate. Then the filtrate was collected, and the filtrate was centrifuged at 10000 rpm for 60 min to collect the precipitate to obtain the original chloroplast. The obtained original chloroplasts were redispersed in a lysis buffer with a concentration of 10mM HEPES (pH8.5) and kept in a 37°C water bath for 5h, and then the lysed solution was centrifuged at 10000rpm for 60min to separate and remove the upper layer Cytoplasmic matrix fluid, collect the precipitate. The precipitate was washed twice with the above-mentioned HEPES buffer solution, redispersed in secondary water with a pH of 8.0, and ultrasonically treated in an ice...

Embodiment 3

[0072] Preparation of nanothylakoids:

[0073] Nanothylakoids were prepared by osmotic lysis. The specific method is as follows: Weigh 100g of fresh spinach leaves and place them in a mortar to grind them into uniform pieces, then add 300mL of a homogeneous buffer solution with a pH of 6.0 to the mortar, mix well, and filter with ten layers of gauze to collect the filtrate. Then the filtrate was collected, and the filtrate was centrifuged at 4000 rpm for 5 minutes to collect the precipitate to obtain the original chloroplast. The obtained original chloroplasts were redispersed in a lysis buffer with a concentration of 10mM HEPES (pH6.8) and kept in a refrigerator at 4°C for 0.5h, and then the lysed solution was centrifuged at 4000rpm for 5min to separate and remove The cytoplasmic matrix fluid in the upper layer was collected and the precipitate was collected. Then the precipitate was washed twice with the above HEPES buffer solution, redispersed in secondary water with a pH...

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Abstract

The invention discloses application of a nanometer capsule body to preparation of anti-tumor medicines. Through the study, the inventor discovers that the nanometer capsule body can promote hydrogen peroxide accumulated in the tumor region to be decomposed into oxygen so that the tumor anoxia can be overcome; meanwhile, the light sensitive characteristic is realized, so that the nanometer capsulebody can be used as a light sensitive agent; the light power anti-tumor operation can be performed, so that the effect of combining the dual functions is achieved. Compared with a single PDT nanometermaterial, the nanometer capsule body has obviously improved PDT treatment effect.

Description

technical field [0001] The invention belongs to the field of biomedical materials; in particular, it relates to the application of a nano thylakoid. Background technique [0002] Cancer is the main killer of human death in the past decades, and the effectiveness of current cancer treatments is severely limited due to the complex tumor microenvironment. Among them, hypoxia is an inherent characteristic of solid tumors, which greatly increases the resistance of tumor therapy, making drug therapy and oxygen-dependent photodynamic therapy less effective. In addition, hydrogen peroxide (H 2 o 2 ) will accumulate in large quantities up to 100μM, while the concentration in normal tissues is only 20nM. Based on these special characteristics of the tumor region, many pharmaceutical agents that regulate the tumor microenvironment to achieve tumor therapeutic effects have been developed, but their biological safety and clinical effect are still open to question. In addition, photod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K41/00A61K47/46A61P35/00
CPCA61K9/5176A61K41/0071
Inventor 刘又年欧阳江陈万松徐群芳
Owner CENT SOUTH UNIV
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