Method for extracting fucoxanthin from brown algae

Abstract

The invention relates to a method for extracting fucoxanthin from brown algae, which is characterized by comprising the following steps: pulping fresh brown algae, adding an organic reagent into the brown algae, and then adding 0.05 to 0.1 mass permillage of antioxidant into the brown algae; leaching the mixture for 22 to 26 hours, and performing suction filtration to obtain leaching solution; leaching the mixture repeatedly until an organic layer is colorless; combining the organic layer liquid of each time into a dark brown bottle; performing rotary evaporation on the organic layer liquid to remove an organic solvent; adding ethyl acetate and water according to a volume ratio of 1:1-2 into an adherence material obtained in a rotary evaporation utensil, oscillating the mixture for layering after dissolving the adherence material, collecting the organic layer, and repeating the operations for 3 to 6 times; combining each organic layer liquid and then performing rotary evaporation and concentration to obtain a total pigment of the brown algae; and separating and purifying the total pigment by using silica gel column chromatography to obtain the high-purity fucoxanthin. The fucoxanthin prepared by the method has high purity, and the method has simple and practical operation, is convenient for industrial production, and provides a basic condition for further development of correlative industrial products and medicines and health care products aiming at the market vacancy of the high-purity fucoxanthin at home and abroad.

Description

technical field [0001] The invention relates to a method for extracting fucoxanthin from brown algae Background technique [0002] There are about 250 genera and 1,500 species of Phaeophyta, most of which are marine except for a few genera and species that live in fresh water. Among the brown algae, there are many economic seaweeds with great economic value. Among them, kelp, wakame, hijiki, etc. are edible and medicinal. Fucoxanthin is also known as fucoxanthin or fucoxanthin, and its molecular formula is C 42 h 58 o 6 , is an important carotenoid contained in brown algae, diatoms, golden algae and yellow-green algae. It can inhibit the activity of DNA replicating enzymes in mammals, and can also be used as a food additive in aquaculture and poultry feeding to enhance the disease resistance of animals and add color to egg yolks. Fucoxanthin is widely distributed in brown algae. [0003] my country is rich in brown algae resources, and the brown algae processing indus...

AI Technical Summary

Problems solved by technology

The above-mentioned extraction method has the following defects: (1) the yield and purity of the proposed fucoxanthin are relatively low

(2) The chemical reagent dimethyl sulfoxide used may cause nausea, vomiting, skin rash and the smell of garlic, onion, and oyster in the skin and exhaled air; the high volatile concentration of dimethyl sulfoxide may

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