Process for production of fucoxanthin, and microalga for use in the process

A technology of fucoxanthin and microalgae, applied in single-cell algae, fermentation, etc., can solve the problems of low productivity, unfavorable preparation cost, difficult to handle scutellaria, etc.

Inactive Publication Date: 2011-04-06
NIPPON SUISAN KAISHA LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the yield of fucoxanthin by the method is as high as 1.1 g per 100 g of dry seaweed, the productivity is low due to the difficulty of handling the scutellum and its slow culture rate, making the production cost unfavorably high

Method used

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  • Process for production of fucoxanthin, and microalga for use in the process
  • Process for production of fucoxanthin, and microalga for use in the process
  • Process for production of fucoxanthin, and microalga for use in the process

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preparation example Construction

[0035] The present invention relates to a method for producing fucoxanthin, which comprises culturing microalgae having a fucoxanthin productivity of 0.5 mg / liter / day or more in a culture medium and isolating and extracting fucoxanthin from the cultured algae.

[0036] The importance of productivity of 0.5 mg / L / day or more

[0037] Microalgae are known to produce fucoxanthin. Fucoxanthin is known as an auxiliary dye with convergence for photosynthesis. However, no examples of research work on the detailed examination of fucoxanthin levels in microalgae are known. Therefore, it is completely unknown how much fucoxanthin is contained therein ("Carotenoid" edited by Shinichi Takaichi, April 2006, pp. 10-48).

[0038] Harvesting fucoxanthin from seaweed involves competition with food, difficult recovery, stable preparation, harsh extraction (presence of polysaccharides), etc. For example, in the case of crocodile buds, the productivity of fucoxanthin was about 0.01 mg / liter / day...

Embodiment 1

[0051] Microalgae of Chrysophyceae

[0052] Ochromonas danica CS-4 strain of Chrysophyceae (available from Microbial Culture Collection, the National Institute for Environmental Studies) was cultured in JM medium (Table 1). The strain was grown at 300 μmol m in 200 ml of JM medium -2 S -1 (14-hour light cycle and 10-hour dark cycle) were cultured for 7 days, and the JM medium was filled into a 500-ml Erlenmeyer flask in a constant temperature water tank at 25°C.

[0053] Table 1

[0054]

Embodiment 2

[0056] Cultivation of Microalgae of the Bromeliaceae

[0057] Pinguiochrysis pyriformis MBIC 10782 strain of Pineappleellaceae (commercially available from Marine Biotechnology Research Institute) was cultured in IMK medium (Table 2). The strain was cultured in 100 ml of IMK medium filled into a 500 ml Erlenmeyer flask capped with Silicosen (manufactured by Shin-Etsu Polymer Co., Ltd.). A 1 ml glass pipette was passed through the Silicosen and the tube was stirred with aeration from its bottom. Cultured in a constant temperature water tank at 25 °C and under aerated conditions at pH control and 300 μmol m -2 S -1 (continuous irradiation) for 7 days. The evolution of the culture over time is shown in figure 1 .

[0058] Table 2

[0059]

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Abstract

Disclosed are: a process for producing fucoxanthin alone or fucoxanthin together with a highly unsaturated fatty acid efficiently, which enables the mass production of fucoxanthin; and a microalga for use in the process. Specifically disclosed are: a process for producing fucoxanthin alone or fucoxanthin together with a highly unsaturated fatty acid, which is characterized by culturing a microalga, more specifically a microalga belonging to the class Pinguiophyceae, Chrysophyceae, Bacillariophyceae or Haptophyceae, and extracting / isolating fucoxanthin alone or fucoxanthin together with the highly unsaturated fatty acid from the cultured microalga; and a microalga for use in the production of each of the above-mentioned fucoxanthin components.

Description

technical field [0001] The present invention relates to a method for producing fucoxanthin or fucoxanthin containing highly unsaturated fatty acids using microalgae, and microalgae for producing fucoxanthin. In particular, the present invention relates to the production of fucoxanthin or fucoxanthin containing highly unsaturated fatty acids using microalgae belonging to the class Pinguiophyceae, Chrysophyceae, Bacillariophyceae or Haptophyceae. Process for fucoxanthin, and microalgae for producing fucoxanthin. Background technique [0002] Fucoxanthin is known to have an anticancer effect, and its applicability as pharmaceuticals and health foods has been examined (Patent Document 1). As a method for producing fucoxanthin, a method including extracting fucoxanthin from, for example, macroalgae (Laminaria genus seaweed) and wakame (Undaria pinnatifida) which are macroalgae is known (Patent Document 2 and 3). Since brown algae belonging to macroalgae such as macroalgae and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/24C12N1/12C12P7/64
CPCC12P23/00C12P7/6427C12N1/12C12P7/6472C12P7/6434
Inventor 泉田仁冲田裕司杉本慎一加藤美砂子
Owner NIPPON SUISAN KAISHA LTD
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