Preparation technology of high purity fucoxanthin

A fucoxanthin and high-purity technology, which is applied in the field of fucoxanthin extraction and purification, can solve the problems of high cost, expensive equipment, and high price, so as to avoid unnecessary losses, save time and manpower, and reduce The effect of purification costs

Inactive Publication Date: 2017-03-08
OCEAN UNIV OF CHINA
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Problems solved by technology

[0004] At present, most of the research on fucoxanthin is still in the laboratory stage. Fucoxanthin products sold in the domestic market are mainly used for research, which are expensive and have low purity. The main reason for this phenomenon is the extraction of fucoxanthin. The immature purification technology, due to the low content of fucoxanthin in raw materials and its special structure, makes it unstable to external conditions such as light, oxygen and heat, and is easy to degrade or isomerize, which gives The extraction and purification of fucoxanthin poses great difficulties
The new extraction technologies reported in the current literature mainly include the addition of enzymes in the pretreatment (Jagan et al.; 2013; Qin Yun et al., 2013; patent CN103232552A, 2012), the application of microwave technology (Xiaohua Xiao et al. 2012; Virginie et al.2010), ultrasonic pretreatment (Chang Zhao et al., 2013), supercritical extraction technology (Armando et al.2013; CN101445496, 2009) and fermentation method (Qu Rongrong, 2011), etc., although these methods have a certain degree of It improves the yield of fucoxanthin, but it also greatly increases the cost of the extraction process, and is limited to specific equipment, which cannot be applied on a large scale
The current purification method, in addition to the traditional silica gel column chromatography, related literature also reported high-speed countercurrent chromatography (Xiaohua Xiao et al. 2012) and preparative high-performance liquid chromatography (patent CN103012327, 2013), although the purified The purity of fucoxanthin is high, but the equipment is expensive, the cost is high, and the amount of fucoxanthin obtained is limited, which is mainly used for laboratory research, thus hindering the development and application of fucoxanthin-related products in the market

Method used

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Examples

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Embodiment 1

[0029] (1) Crush the dried wakame into dry wakame powder (50-100 mesh);

[0030] (2) Take 100g of dried wakame powder, add 1.5L of 70% ethanol aqueous solution, stir evenly, and stand at 40°C for 12.0h in the dark;

[0031] (3) Suction filter the seaweed extract, extract the residue a second time according to the operation of step (2), and finally discard the residue, and mix the algae powder extract twice;

[0032] (4) Pretreat the AB-8 resin with 75% ethanol aqueous solution for 24 hours, then fully rinse with distilled water, then rinse the AB-8 resin with 75% ethanol solution for 3~4 times, so that the resin is immersed in 75% ethanol aqueous solution, and finally the AB-8 macroporous adsorption resin is wet-packed, and 75% ethanol aqueous solution is balanced;

[0033] (5) Load the sample extract at a rate of 0.5BV / h, desorb with absolute ethanol at a rate of 1.0BV / h, collect the eluate continuously, and perform HPLC chromatographic analysis;

[0034] (6) Concentrate th...

Embodiment 2

[0037] (1) 3kg of dried wakame is crushed into dry wakame powder;

[0038] (2) Add 45L of 75% ethanol aqueous solution into a 50L reaction vessel, add the dry wakame powder into it, stir for 30min, and extract at 45°C in the dark for 16h;

[0039] (3) Suction and filter the seaweed extract, repeat the extraction of the residue, the same as step (2), and finally mix the two extracts;

[0040] (4) Pretreat the AB-8 resin according to the steps in (4) in Example 1, and wet-pack the column (10×75cm), the column height after equilibrium is 56cm, and the volume of 1 column is 4.4L;

[0041] (5) Automatically load the sample at a speed of 70ml / min, elute with absolute ethanol, collect the orange eluate and mix it evenly, and analyze it by HPLC;

[0042] (6) Concentrate the eluent under reduced pressure below 40°C to a certain amount, and blow dry with nitrogen to obtain 378g of red paste, containing 10.3g of fucoxanthin.

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Abstract

The present invention relates to a technology for extracting high purity fucoxanthin from algae. The technology comprises steps of pretreatment of algae, solvent extraction of the fucoxanthin, and purification of the fucoxanthin. The technology of the invention solves the problems of low product purity and solvent residues of the extracted fucoxanthin in the prior art. Because it is easy to get raw materials employed in the technology of the invention, the extracted solvent is recoverable, the extraction yield is high, purification materials can be re-used, and the extraction and purification processes are simple, the technology of the invention has low cost and high efficiency and lays the foundation for the industrial production of fucoxanthin related products.

Description

technical field [0001] The present invention relates to the extraction and purification of fucoxanthin, in particular to the technology of extracting fucoxanthin from a kind of brown algae and diatoms in offshore waters, such as wakame, and using macroporous adsorption resin to purify. Background technique [0002] Fucoxanthin (fucoxanthin) is the most abundant pigment among carotenoids, and its production accounts for about 10% of the total production of carotenoids in nature (Hosokawa et al., 2009). It mainly exists in brown algae and diatoms in coastal waters Among them, it is its characteristic pigment, combined with protein, and forms fucoxanthin-chlorophyll a-protein complex together with chlorophyll a in thylakoids, which plays the role of light harvesting and energy conversion (Caron et al., 1996 ). [0003] In 1914, fucoxanthin was first isolated in crystal form from the brown algae Fucus, Dictyota and Laminaria by Willstätter and Page. Subsequently, its complete ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C07D301/32
CPCC07D301/32C07D303/32
Inventor 汪东风陈晓利张莉汪曙晖
Owner OCEAN UNIV OF CHINA
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