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Method for extracting and purifying fucoxanthin from brown alga

A technology of fucoxanthin and purification method in brown algae, applied in the direction of organic chemistry, can solve the problems of long operation time, expensive, high processing cost, etc., achieve the effect of economical price, reduce residue, and increase added value

Inactive Publication Date: 2013-02-06
THE FIRST INST OF OCEANOGRAPHY SOA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Second, the cost of separation is too high, and the environmental pollution in the separation project is serious
Dimethyl sulfoxide has a high boiling point and is relatively expensive, and the follow-up treatment cost is very high; and the method adopted in the CN101565411 patent has a long operation time and low product yield

Method used

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  • Method for extracting and purifying fucoxanthin from brown alga
  • Method for extracting and purifying fucoxanthin from brown alga
  • Method for extracting and purifying fucoxanthin from brown alga

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Extract and purify fucoxanthin from kelp

[0027] 1. Preparation and screening of kelp crude extract

[0028] Wash and dry 10kg of kelp, put it in an oven, and dry it at 45°C to obtain a dry weight of 346.49g of kelp. The dried kelp was crushed and ground into powder, and ultrasonically extracted with acetone:methanol=3:1 mixture at 45°C for 4 hours. Leach for 3 times, filter the extract, and evaporate the clear liquid to dryness under vacuum at 45°C to obtain the crude extract of kelp.

[0029] Edible petroleum ether, acetone and methanol were sequentially added to the crude kelp extract for dissolution to obtain samples 0316-1-3.

[0030] Use an ultraviolet spectrophotometer to scan the 0316-1~3 samples at a wavelength of 300~500nm, and use the corresponding pure solvent as a blank reference. The results show that the sample 0316-3, that is, the methanol extract has the largest absorption peak at 450nm, and the content of carotenoids and their derivat...

Embodiment 2

[0034] Example 2: Extraction and purification of fucoxanthin from macroalgae

[0035] 1. Preparation of macroalgae crude extract

[0036] Wash and dry 10kg macroalgae, put them in an oven, and dry them at 45°C. The dried macroalgae was crushed and ground into powder, and ultrasonically extracted with a mixture of acetone:methanol=3:1 at 45°C for 4 hours. Extracted 3 times, filtered the extract, and evaporated to dryness in a vacuum at 45°C to obtain a dry crude sample of macroalgae. Add 20ml of methanol to dissolve the dry sample and use it as a sample for column chromatography.

[0037] 2. Separation and purification of fucoxanthin

[0038] Firstly, the dry method is used to pack the column, and the column filler is diatomaceous earth. Add a small amount of diatomaceous earth to the samples obtained in the above steps, mix them dry and load them. Use edible petroleum ether and acetone with a volume ratio of 1:0.75 as the eluent for gradient elution, and finally use 1L of...

Embodiment 3

[0040] Embodiment 3: Extract and purify fucoxanthin from Sargassum

[0041] One, the preparation of Sargassum crude extract

[0042] Wash and dry 10kg of sargassum, place in an oven, and dry at 45°C. The dried Sargassum was crushed and ground into powder, and ultrasonically extracted with a mixture of acetone:methanol=3:1 at 45°C for 4 hours. Leach for 3 times, filter the extract, and evaporate the clear liquid to dryness in a vacuum at 45° C. to obtain a dry crude sample of Sargassum. Add 20ml of methanol to dissolve the dry sample and use it as a sample for column chromatography.

[0043] 2. Separation and purification of fucoxanthin

[0044]Firstly, the dry method is used to pack the column, and the column filler is diatomaceous earth. Add a small amount of diatomaceous earth to the samples obtained in the above steps, mix them dry and load them. Use edible petroleum ether and acetone with a volume ratio of 1:0.52 as the eluent for gradient elution, and finally use 1L ...

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Abstract

The invention relates to a method for extracting and purifying fucoxanthin from brown alga, which comprises the steps of primary extraction sample preparation, methanol dissolution, column chromatography separation and HPLC (high performance liquid chromatography) purification. The invention provides a fucoxanthin preparation scheme which has the advantages of high safety, low toxicity, high economy and environmental protection. The invention adopts kieselguhr as a column filler to separate the fucoxanthin for the first time. The kieselguhr has the characteristics of low price and environmental protection. Meanwhile, edible petroleum ether is utilized for separation, thereby lowering the residues of toxic substances in the final product. After the kieselguhr is separated, the fucoxanthin health product raw material which is cheap, safe and healthy can be obtained. The fucoxanthin is separated and purified by HPLC to obtain the chromatographically pure fucoxanthin. The invention provides the safe low-toxicity separation technique for satisfying the high-purity fucoxanthin market at home and abroad, greatly enhances the added value of the brown alga aquatic products, and thus, has important economic value.

Description

technical field [0001] The invention belongs to the technical field of marine biological natural products, and in particular relates to a method for extracting and purifying fucoxanthin in brown algae. Background technique [0002] Since the signing of the United Nations Convention on the Law of the Sea in 1982, oceanic countries around the world have taken the development and utilization of marine resources as a national strategic decision, and vigorously promoted the development of related scientific research and technology industries. As an important branch of research, marine natural products provide a large number of active substances with medicinal value. [0003] As one of the marine natural active substances, fucoxanthin is widely distributed in the marine environment, and its content accounts for about 10% of the total natural content of carotenoids. However, unlike carotenoids such as β-carotene, fucoxanthin is the first molecule isolated from brown algae with a s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C07D301/32
Inventor 陈颢秦松张璇李培海张学雷张克诚
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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