Method for extracting high-purity fucoxanthin from seaweeds

A fucoxanthin, high-purity technology, applied in organic chemistry and other directions, can solve the problems of high purification cost and environmental pollution, and achieve the effect of good separation effect, high purity and less equipment and equipment

Active Publication Date: 2014-06-04
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the extraction and purification process of high-purity fucoxanthin mainly relies on chromatographic techniques, such as a method for isolating fucoxanthin from seaweed (publication number CN 1706836 A), and a method for purifying fucoxanthin The method (publication number is CN 101735178A), the name is a method for enzymatically preparing fucoidan and fucoxanthin (publication number is CN 103232552 A), in the above patent, the purification process of high-purity fucoxanthin Both adopt chromatographic methods, not only the purification cost is high, but also the environmental pollution in the purification project is serious. Therefore, there is an urgent need for an extraction and purification technology with simple extraction process, no pollution, and suitable for industrial production, so as to meet the demand for high-purity fucoidan in domestic and foreign markets. Flavin needs

Method used

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  • Method for extracting high-purity fucoxanthin from seaweeds
  • Method for extracting high-purity fucoxanthin from seaweeds
  • Method for extracting high-purity fucoxanthin from seaweeds

Examples

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Effect test

Embodiment 1

[0048] Wash fresh copper algae 3 times with seawater to remove surface impurities, then wash 2 times with distilled water to remove salt ions on the surface of the algae, and then use absorbent cotton to dry the surface water. Weigh 200g of copper algae into a 1000mL conical beaker, add 600mL of 80% (V / V) ethanol aqueous solution, seal the mouth of the conical beaker with two layers of safety film, extract in the dark for 12 hours, and filter. Continue to add 600mL of 80% (V / V) ethanol aqueous solution to the copper algae that has undergone the above extraction treatment, seal the mouth of the triangular beaker with two layers of safety film, extract in the dark for 12 hours, and filter. The copper algae was repeatedly extracted in the dark until the extract was colorless;

[0049] Combine the above extracts and pour them into a brown bottle, and evaporate in vacuum at 40°C. Pay attention to the changes of the solution when concentrating. When a large amount of black and green...

Embodiment 2

[0053] Thaw frozen sargassum at room temperature, wash 5 times with seawater to remove surface impurities, then wash 3 times with distilled water to remove salt ions on the surface of the algae, and then dry the surface with absorbent paper. Weigh 200g of Sargassum and put it into a 1000mL beaker, add 700mL of 75% (V / V) ethanol aqueous solution, seal the mouth of the beaker with two layers of safety film, extract in the dark for 8 hours, filter, and continue to add Sargassum Add 600mL of 80% (V / V) ethanol water solution again, seal the mouth of the triangular beaker with two layers of safety film, extract in the dark for 12 hours, and filter. The sargassum was repeatedly extracted in the dark until the extract was colorless;

[0054] Combine the above extracts and pour them into a brown bottle, and evaporate in vacuum at 38°C. Pay attention to the changes of the solution when concentrating. When a large amount of black and green precipitates gradually appear in the solution, f...

Embodiment 3

[0057] The same as the above-mentioned Example 1, the difference is that: copper algae dark leaching time is 24 hours; vacuum rotary evaporation temperature is 15 ° C; organic reagent is acetone; the ratio of copper algae to organic reagent is 200g:800ml.

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Abstract

The invention discloses a method for extracting high-purity fucoxanthin from seaweeds. The method is characterized by comprising the following steps: cleaning the seaweeds by using the distilled water so as to remove saline ions on the surfaces of the seaweeds, and then removing moisture on the surfaces of the seaweeds; then adding an organic reagent to the seaweeds, digesting in the dark for 8-24 hours, and carrying out suction filtration so as to obtain a leaching liquid; repeatedly digesting the leaching liquid till the leaching liquid is colorless, and combining each leaching liquid into a brown bottle; finally carrying out rotary evaporation for a period of time till black green precipitates occur in a liquid, and filtering so as to remove pigment and impurities; and continuously carrying out rotary evaporation till red solids are separated out in the liquid, and filtering so as to obtain the red solids which are the high-purity fucoxanthin. The method has the advantages that the safely healthy high-purity fucoxanthin raw material is obtained for the first time under the condition that no chromatographic techniques are used for separation, and the preparation process is safe, harmfulless, economic and environment-friendly.

Description

technical field [0001] The invention relates to a method for extracting fucoxanthin, in particular to a method for extracting high-purity fucoxanthin from seaweed. Background technique [0002] Fucoxanthin (fucoxanthin), its molecular formula is: [0003] [0004] Also known as fucusanthin, its crystal or solution is red under visible light. It is one of the important members of the carotenoid family. As an active secondary metabolite of seaweed, it is widely distributed in the marine environment and is a brown algae in algae. The main carotenoid components of seaweeds of the class Diatomaceae, Chrysophyceae, and Dinophyceae, especially the characteristic carotenoid components of seaweeds of the class Phaeophyceae. Fucoxanthin is the first molecule isolated from brown algae with a special allene carbon skeleton. It has a strong antioxidant capacity and is a strong chain-cutting antioxidant. This antioxidant effect can effectively Eliminate excess free radicals and singl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C07D301/32
CPCC07D301/32C07D303/32
Inventor 张金荣严小军陈海敏何山骆其君许亚如
Owner NINGBO UNIV
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