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Method for producing fucoxanthin through light culture of nitzchia laevis

A technology of fucoxanthin and light culture, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the difficulties of industrial application, optimization of uncultivated conditions, and low yield of fucoxanthin in biomass concentration and other issues to achieve the effect of shortening the production cycle, reducing production costs, and reducing pollution risks

Active Publication Date: 2017-09-01
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Guo and the designer in this application obtained several strains of diatoms capable of synthesizing fucoxanthin through screening, and found that the fucoxanthin content of Cyclotella cryptica CCMP 333 can reach 0.77%, which is the highest among more than ten strains of microalgae. Suitable for fucoxanthin production algal strains, but its biomass concentration and fucoxanthin yield are low, and industrial application is still difficult
Under the culture conditions used, Nitzschia laevis UTEX 2047 produces fucoxanthin under photoautotrophic conditions, and its content (accounting for cell dry weight) is as high as 0.7%, but the biomass concentration is <0.2g / L, and the yield is only is 0.06mg / (L·d), but this paper did not optimize its culture conditions (Guo, et al., 2016)

Method used

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  • Method for producing fucoxanthin through light culture of nitzchia laevis
  • Method for producing fucoxanthin through light culture of nitzchia laevis
  • Method for producing fucoxanthin through light culture of nitzchia laevis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Autotrophic culture of Rhizobium smoothie

[0052] The production strain was Nitzschia laevis UTEX 2047 (purchased from the Microalgae Collection of the University of Texas at Austin, Culture Collection of Algae at The University of Texas at Austin, UTEX for short).

[0053] The process steps are as follows:

[0054] A, by inoculating the rhizoma smoothie of the activated bacterial classification in the sterile seed medium that is placed in the shake flask, heterotrophic culture is made for 3 days to make the seed liquid, and the rhomboid rhizomes cell is in the logarithmic growth phase in the seed liquid;

[0055] B. Take a 250mL conical flask as a culture vessel, transfer into 100mL of fermentation medium and sterilize. According to the inoculum volume ratio of 10%, the seed liquid in step A is inserted into the sterile fermentation medium for autotrophic culture on a shaker to prepare a fermentation broth. The culture conditions are as follows: the light intensity i...

Embodiment 2

[0062] Polyculture of Rhizobium smoothie

[0063] The difference between Example 2 and Example 1 is that the seed medium and the fermentation medium in Example 2 also contain the following raw materials: glucose 5 g / L, and other contents are the same as those in Example 1.

[0064] The effect analysis of embodiment 1,2:

[0065] Compared with the autotrophic and polyculture conditions, the growth state of the polyculture was better, the polyculture biomass was up to 2.68g / L, and the autotrophy was only 0.70g / L (see figure 1 ). like figure 2 , the autotrophic fucoxanthin content was 1.0%, and the polyculture was up to 1.2%. The calculated yield of fucoxanthin in polyculture was higher than that in autotrophy, and the optimal yield was 4.59 mg / (L·d).

Embodiment 3

[0067] Polyculture of Rhizobium smoothie

[0068] The difference between Example 3 and Example 2 is that the seed medium and the fermentation medium also contain the raw materials of the following content: glucose 20g / L, and the production strain selects Nitzschia laevis CCMP 559 (purchased from U.S. Marine Microorganisms). Algae and Microorganisms Collection Center, National Center for Marine Algae and Microbiota, referred to as NCMA), other contents are the same as Example 2.

[0069] Effect analysis of embodiment 3:

[0070] The production strain in this example has a good growth state, and the biomass is up to 2.88g / L. The autotrophic fucoxanthin content was 0.9%. It is calculated that the yield of fucoxanthin produced by fermentation of the production strain in this example is higher than that of autotrophy, and the optimal yield is 4.32 mg / (L·d).

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Abstract

The invention belongs to culture of microorganisms, in particular to a method for producing fucoxanthin through light culture of nitzchia laevis. The method comprises the following steps: preparing a seed solution; performing fermentation culture; extracting the fucoxanthin from fermentation liquor obtained after fermentation. By adopting the method, the problems of low biomass concentration, low fucoxanthin yield, low fucoxanthin content and the like of the nitzchia laevis under a phototrophic condition in the prior art are solved. Prepared nitzchia laevis dry powder has high fucoxanthin content and high fucoxanthin yield, the fucoxanthin produced by the method is safer than fucoxanthin derived from a large-sized seaweed source, the production efficiency is increased, the production cost is lowered, and meanwhile the pollution risk in a culturing process is lowered greatly.

Description

technical field [0001] The invention belongs to the cultivation of microorganisms, in particular to a method for producing fucoxanthin by cultivating Pseudomonas platypus by light. Background technique [0002] Fucoxanthin, also known as fucoxanthin and fucoidin, mainly comes from macroalgae, diatoms and golden algae and other macroalgae and microalgae. It is a natural carotenoid and participates in the photosystem in photosynthesis. II's response. Fucoxanthin is an active molecule with strong antioxidant properties due to its unique allene structure. In recent years, studies have found that fucoxanthin has a variety of functional activities in cells, animals and humans, including antioxidant, anti-inflammatory, anti-cancer, anti-obesity, anti-diabetic, anti-angiogenesis and anti-malarial physiological activities. It has a protective effect on organs such as liver, brain blood vessels, bones, skin and eyes. Combining the above functions, fucoxanthin is a natural product w...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/89
CPCC12P23/00
Inventor 陈峰卢雪刘宾
Owner PEKING UNIV
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