Pretreatment method of brown alga samples for fucoxanthin extraction

A technology of fucoxanthin and brown algae, which is applied in the field of pretreatment of brown algae samples, can solve the problems of long operation time, difficulty in realizing the industrial production of fucoxanthin products, and high operating costs, so as to avoid damage and decomposition, good yield and Quality, evenly distributed effect inside and outside

Active Publication Date: 2015-10-28
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can maintain the active ingredients in brown algae, but due to the long operation time and high operation cost, it is difficult to realize the industrial production of fucoxanthin products

Method used

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  • Pretreatment method of brown alga samples for fucoxanthin extraction
  • Pretreatment method of brown alga samples for fucoxanthin extraction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Freeze the fresh copper algae at a temperature of -20°C, take it out after freezing for 3 days, and thaw it in the air at room temperature, and complete the first freeze-thaw process after the central temperature of the copper algae reaches 0°C; machine, 1000rpm; centrifuge for 10 minutes to remove water; then freeze the sample at a temperature of -20°C, take it out after freezing for 3 days, put it into a centrifuge, 1000rpm; centrifuge for 10 minutes to remove water. Repeat the third freeze-thaw process and centrifugation process. The samples before freezing and thawing, the samples after the first freeze-thaw and centrifugation, the samples after the second freeze-thaw and centrifugation, the samples of copper algae after the third freeze-thaw and centrifugation were taken respectively, and the water content was determined. Determination of fucoxanthin content, calculation of fucoxanthin yield after freezing and thawing. The measurement results are shown in the tabl...

Embodiment 2

[0029] Freeze the fresh sargassum at -10°C, take it out after freezing for 30 days, and thaw it in the air at room temperature, and complete the first freeze-thaw process when the central temperature of the copper algae reaches 2°C; Put it into a centrifuge at 2000rpm; centrifuge for 8 minutes to remove water; then freeze the sample at a temperature of -10°C, take it out after freezing for 30 days, put it in a centrifuge at 2000rpm; centrifuge for 8 minutes to remove water. Complete the fourth freeze-thaw process and centrifugation process repeatedly. Samples were taken before freeze-thaw, after the first freeze-thaw and centrifugation, after the second freeze-thaw and centrifugation, after the third freeze-thaw and centrifugation, and after the fourth freeze-thaw and centrifugation. The sargassum samples were used to measure the water content of the sargassum, the fucoxanthin content in the sargassum, and calculate the yield of fucoxanthin after freezing and thawing. The mea...

Embodiment 3

[0032] Same as the above-mentioned Example 1, the difference is that the fresh sea-tangle is repeatedly frozen and thawed 5 times at a temperature of -80° C., the centrifugation speed is 3000 rpm, and the centrifugation time is 7 minutes.

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Abstract

The invention discloses a pretreatment method of brown alga samples for fucoaxanthin extraction. The pretreatment method is characterized by concretely including the steps that step1, the fresh brown algae are frozen for 1-30 days at the temperature of below 5 DEG C-80 DEG C and then taken out, air thawing is performed at room temperature until the center temperature of the brown algae reaches 0 DEG C-2 DEG C, and then the freeze thawing process is completed; the brown algae are placed into a centrifugal machine and centrifuged for 3-10 minutes at the rotation speed of 800 rpm-10000 rpm, and then water is removed; step2, the step1 is repeated 1-4 times, and a solvent extraction and precipitation method is adopted for preparing high-purity fucoxanthin from the brown algae after freeze thawing treatment is performed. The pretreatment method has the advantages that the fucoxanthin and other effective components in the brown algae can be effectively prevented from being damaged and decomposed, the difference between the water content of the surfaces of the brown alga samples and the water content inside the brown alga samples is small, inside and outside distribution is even, and the water content of the treated brown algae is 50-90%.

Description

technical field [0001] The invention relates to a pretreatment method of brown algae samples, in particular to a pretreatment method of brown algae samples for extracting fucoxanthin. Background technique [0002] Fucoxanthin (fucoxanthin), also known as fucoxanthin and fucoxanthin, is an important carotenoid contained in brown algae, diatoms, golden algae and yellow-green algae. It participates in the photochemical system of photosynthesis. The capsule participates in photosynthesis and plays the role of light harvesting and light transmission. According to reports, fucoxanthin has a variety of biological effects, such as anti-cancer, anti-inflammation, prevention of diabetes, prevention of obesity, anti-oxidation, etc. It has potential drug development value and can be used as medicine, food, aquatic product bait, poultry and livestock Feed etc. Cosmetics and fucoxanthin soft capsules with fucoxanthin as the main ingredient are already on the market in the world. In rec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C07D301/32
CPCC07D301/32C07D303/32
Inventor 赵诗瑶张金荣娄永江周成旭严小军陈海敏骆其君朱鹏何山王杰
Owner NINGBO UNIV
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