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Method for producing fucoxanthin by heterotrophic culture of smooth nitzschia

A technology of heterotrophic culture and fucoxanthin is applied in the field of fucoxanthin production by using heterotrophic culture of Rhizoma smoothie, which can solve problems such as optimization of culture conditions, and achieve the advantages of reducing production cost, reducing pollution risk and improving production efficiency. Effect

Inactive Publication Date: 2017-08-29
PEKING UNIV
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  • Description
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  • Application Information

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Problems solved by technology

However, Nitzschia laevis UTEX 2047 produces fucoxanthin under photoautotrophic conditions, and its content (accounting for dry cell weight) is as high as 0.7%, but the biomass concentration is <0.2g / L, and the yield is only 0.06mg / (L·d), and this paper did not optimize the culture conditions (Guo, et al., 2016)

Method used

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  • Method for producing fucoxanthin by heterotrophic culture of smooth nitzschia
  • Method for producing fucoxanthin by heterotrophic culture of smooth nitzschia

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Embodiment 1

[0054] Heterotrophic culture of Nitzschia smoothis

[0055] Nitzschia laevis UTEX 2047 (purchased from the Microalgae Collection of the University of Texas at Austin, Culture Collection of Algae at The University of Texas at Austin, UTEX for short) was selected as the production strain in this example.

[0056] A. Preparation of seed solution

[0057] Put the activated Nitzschia smoothii in a sterile seed medium for heterotrophic culture for 3-11 days to make a seed solution, so that the Nitzkiplankton cells are in the logarithmic growth phase;

[0058] B. Fermentation culture

[0059] Transfer the seed liquid in step A into a sterile fermentation medium according to the volume ratio of 10% inoculum to prepare a fermentation liquid on a shaker, and cultivate it under the conditions of a culture temperature of 23°C and a rotation speed of 150 rpm 5 days;

[0060] Described seed culture medium and fermentation medium comprise the raw material of following content scope:

[0...

Embodiment 2

[0066] Embodiment 2: Heterotrophic cultivation of Nitzschia smoothis

[0067]With a 250mL Erlenmeyer flask as a culture vessel, 100mL of fermentation medium was transferred, and the inoculation amount of 10% by volume was inserted into the fermentation medium for shaker culture. The culture conditions are as follows: the rotation speed of the shaker is 150 rpm, the temperature is 23° C., and the culture time is 5 days.

[0068] The fermentation medium and seed medium also include the following raw materials: NaNO 3 1g / L, all the other contents are with embodiment 1.

[0069] The difference between Example 3-5 and Example 2 is that the initial glucose concentration in Example 3-5 is 10g / L, 20g / L and 40g / L respectively, and the culture time is extended to 7, 9, 11 days respectively.

[0070] The effect analysis of embodiment 2-5:

[0071] see figure 2 and image 3 , different initial concentrations of glucose had a great influence on the growth and fucoxanthin content of ...

Embodiment 6-11

[0073] The difference between Examples 6-11 and Example 1 is that the nitrogen sources in Examples 6-11 are respectively 2g / L NaNO 3 、KNO 3 , NH 4 Cl, urea, peptone and yeast extract. All the other contents are the same as in Example 1.

[0074] The effect analysis of embodiment 6-11:

[0075] see Figure 4 , only 2g / L of sodium nitrate is used to detect that the content of fucoxanthin in the freeze-dried algal powder can be as high as 0.91%, and the content of fucoxanthin is greatly increased. Nitrogen sources Only use inorganic nitrogen sources—sodium nitrate or potassium nitrate can get higher content of fucoxanthin, while the content of fucoxanthin obtained when only organic nitrogen sources peptone and yeast extract are used is lower than when only inorganic nitrogen sources are used Fucoxanthin content.

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Abstract

The invention belongs to culture of microorganisms, and particularly relates to a method for producing fucoxanthin by heterotrophic culture of smooth nitzschia. The method comprises the following steps: preparation of a seed solution, fermental cultivation, extraction of the fucoxanthin from fermentation liquor after fermentation is finished and the like. The problem that the biomass concentration of the smooth nitzschia, the yield of the fucoxanthin and the content of the fucoxanthin are low under the condition of heterotrophic culture in the prior art is solved. The method has the advantages that the content of fucoxanthin in prepared dry smooth nitzschia powder is high, the yield of the fucoxanthin is high, the fucoxanthin is safer than fucoxanthin from large seaweeds, and while the production efficiency is improved and the production cost is reduced, pollution risks in a culture process is greatly reduced.

Description

technical field [0001] The invention belongs to the cultivation of microorganisms, and in particular refers to a method for producing fucoxanthin by using heterotrophic cultivation of Nishina smoothis. Background technique [0002] Fucoxanthin (Fucoxanthin), also known as fucoxanthin and fucoidin, mainly comes from large algae and microalgae such as algae, diatoms, and golden algae. It is a natural carotenoid that participates in the photosystem of photosynthesis II's response. Because of its unique allene structure, fucoxanthin is an active molecule with strong antioxidant properties. In recent years, studies have found that fucoxanthin has a variety of functional activities in cells, animals and humans, including anti-oxidation, anti-inflammation, anti-cancer, anti-obesity, anti-diabetes, anti-angiogenesis and anti-malarial and other physiological activities, and It has a protective effect on organs such as liver, brain blood vessels, bones, skin and eyes. Based on the ...

Claims

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Application Information

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IPC IPC(8): C12P17/02C12N1/12C12R1/89
CPCC12N1/12C12P17/02
Inventor 陈峰卢雪刘宾
Owner PEKING UNIV
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